Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
World’s cleanest buffer for all cell and molecular biology experiments
Description
PBS Blocking Buffer with Casein (1%) is a commonly used reagent in immunoassays such as Western blotting, ELISA, and immunohistochemistry. It is designed to block non-specific binding sites on membranes and microplates, thereby enhancing the specificity and sensitivity of these assays. The buffer contains 1% casein, a protein derived from milk, which is known for its effective blocking properties. Casein is often purified and sometimes chemically modified to enhance its blocking efficiency. Phosphate Buffered Saline (PBS) provides a stable pH environment, essential for maintaining the integrity of proteins and antibodies during assays. Casein blocking buffers are particularly recommended for applications using biotin-avidin complexes, as they provide lower backgrounds than buffers containing non-fat milk.
Applications
Western Blotting: Used to block non-specific sites on membranes, ensuring that antibodies bind specifically to target proteins.
ELISA (Enzyme-Linked Immunosorbent Assay): Reduces background noise by preventing non-specific interactions between assay components.
Immunohistochemistry (IHC): Prevents non-specific binding of antibodies to tissue sections, improving the clarity and accuracy of staining.
Advantages:
Optimal Concentration: The 1% concentration of casein is considered optimal for most applications, providing a balance between effective blocking and maintaining assay sensitivity.
Reduced Cross-Reactivity: As a single-protein blocking agent, casein reduces the risk of cross-reactions with assay components compared to complex mixtures like serum or milk.
Reproducibility and Low Variability: The use of highly purified and modified casein ensures consistent results, making it suitable for high-precision assays.
Composition:
Ingredients
Concentration
Casein
1%
Sodium Chloride
137 mM
Potassium Chloride
2.7 mM
Sodium Phosphate Dibasic Anhydrous
10.1 mM
Potassium Phosphate Monobasic Anhydrous
1.76 mM
Quality Control:
Appearance: Opaque Liquid.
pH: 7.4
Sterility: Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
DNase activity: None was detected after incubation of plasmid DNA and this product for 18 hr at room temperature.
RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
Storage: -20 ℃ Long term * bringing CPBS to room temperature or 37 °C before use minimizes unwanted temperature?induced variables.
Shelf life: 1 year
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
DiagnoCine Precision Sterile buffers are temperature sterilized and filtered-sterilized with a 0.1-micron filtration Twice. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
