FluxMPS™ CMRL 1066 Medium w/o Sodium Bicarbonate: 1X Liquid
FluxMPS™ CMRL 1066 Medium w/o Sodium Bicarbonate: 1X Liquid
FluxMPS™ CMRL 1066 Medium w/o Sodium Bicarbonate: 1X Liquid is a Microfluidics Suitable, ultra-filtered cell culture medium engineered for organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and microphysiological system (MPS) applications. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.
- Quadruple-stage nano-filtration reaching a 0.04 µm final pore size (0.1 µm ×2 + 0.04 µm ×2)
- Endotoxin release specification: less than 0.05 EU/mL (USP <85> BET)
- CMRL 1066 base formulation with 1000 mg/L (1.0 g/L) glucose and 100 mg/L L-glutamine
- Formulated without sodium bicarbonate and without HEPES — buffering system to be added per your application
- Contains phenol red (21.24 mg/L) as a pH indicator; orange-red colored, clear solution
- Manufactured under an ISO 13485:2016 quality management system; final packaging and QC completed at Diagnocine, Totowa, NJ
- Custom formulations available — pH, glucose, salts, and nutrient composition on request
- Glucose1000 mg/L (1.0 g/L)
- L-Glutamine100 mg/L
- Sodium PyruvateNot present
- HEPESNot present
- pH (USP <791>)7.4
- Osmolality (USP <785>)Contact for specification
- Endotoxin (USP <85>)less than 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2
- Storage2–8°C, protect from light
- Shelf Life12 months from date of manufacture, unopened
Engineered where standard media fails
Conventional 0.22 µm-filtered media carry mycoplasma-sized particulates (0.2–0.3 µm), subvisible debris, and variable endotoxin load that accumulate inside microchannels — corrupting biosensor readings and shortening device lifetimes. FluxMPS™ is built to reduce these failure modes at the source.
Microchannel-safe purity
0.04 µm final filter stage retains particles down to sub-mycoplasma size; USP <788> Method 1 (light obscuration) particulate compliance verified per lot.
Total metabolic control
Defined glucose and L-glutamine levels support precise nutrient-controlled experiments and metabolic flux studies.
Ultrapure-grade water
Prepared with Type 1 water (18.2 MΩ·cm, ASTM D1193 / ISO 3696 Type I), with controlled trace-metal and total organic carbon (TOC) levels.
Low background for imaging
Quadruple-stage filtration reduces particulate baseline, supporting confocal microscopy, live-cell biosensors, and TEER measurements.
Rich, stable nutrient profile
Micro-batch precision manufacturing locks in amino acid and vitamin concentrations, supporting lot-to-lot reproducibility for long-term perfusion studies.
Customization on demand
pH, glucose concentration, salts, HEPES, and full nutrient composition available on request. Contact support@diagnocine.com.
Quadruple-stage filtration system
FluxMPS™ CMRL 1066 Medium w/o Sodium Bicarbonate: 1X Liquid is processed through a four-stage serial filtration sequence that reaches a 0.04 µm final pore size — addressing mycoplasma-sized particulates and subvisible particulate load beyond what single-pass 0.22 µm filtration can achieve.
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1
0.1 µm Prefiltration I
Removes large particulates and aggregates; protects the first 0.04 µm final filter cartridge.
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2
0.04 µm Final filtration I
Fine particulate retention at a pore size below the typical mycoplasma diameter (0.2–0.3 µm).
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3
0.1 µm Prefiltration II
Second dedicated prefilter, protecting the second 0.04 µm final filter cartridge for full-redundancy processing.
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4
0.04 µm Final filtration II — Polish
Ultimate polishing filter; aseptic fill & finish performed in a validated ISO Class 5 (Class 100) laminar-flow workstation.
Performance vs. conventional media
By reaching a 0.04 µm final pore size across four sequential stages, FluxMPS™ delivers approximately 5× cleaner media by particulate count compared to single-pass 0.22 µm filtration, verified per USP <788> (light obscuration, Method 1) on every production lot.
© Diagnocine® — DCP-CMRL-B1X
Designed for next-generation cell culture platforms
FluxMPS™ CMRL 1066 Medium w/o Sodium Bicarbonate: 1X Liquid is suited for use across organ-on-a-chip, metabolic research, live-cell imaging, and primary cell models where particulate contamination and endotoxin variation are unacceptable.
Automated Bioreactors & Robotics
An optional 0.01 µm (10 nm) MPS Grade variant is available for automated bioreactor and robotic perfusion systems requiring the ultimate particulate exclusion.
- Total Particulate Exclusion: 10 nm filtration for nanoscale valve and sensor protection
- Valve & Sensor Protection: prevents particulate-induced blockage in precision fluidic systems
- Extended Perfusion Stability: maintains flow rate consistency across multi-week automated runs
Inquiry Required: The 0.01 µm MPS Grade variant is available by special order. Contact support@diagnocine.com to request this variant.
Micro Physiological System (MPS) & Chip
Ultra-filtered formulation supports laminar flow integrity across complex chip geometries.
Warburg Effect & Metabolic Research
Defined glucose level and low-endotoxin release specification support metabolic flux analysis.
iPSC-Derived Models
Ultrapure formulation supports sensitive iPSC differentiation protocols where endotoxin and particulates cause off-target effects.
Endothelial & Primary Cells
Microchannel-safe purity supports endothelial barrier integrity and TEER measurements in perfusion models.
Metabolic Flux Analysis
Defined base supports isotope tracing (13C, NMR) for metabolic flux analysis. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.
Microscopy & Optical Sensing
Low particulate load supports high-content confocal imaging and optical biosensor integration.
Lot-release quality parameters
Every production lot of FluxMPS™ CMRL 1066 Medium w/o Sodium Bicarbonate: 1X Liquid undergoes the quality-release battery listed below before shipment.
| Parameter | Specification |
|---|---|
| Formulation | CMRL 1066 w/o Sodium Bicarbonate — contains Glucose, L-Glutamine, Calcium, Magnesium, Phenol Red; without Sodium Bicarbonate, HEPES, Sodium Pyruvate |
| Appearance | Orange-Red colored, clear solution |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | Contact for specification |
| Glucose | 1000 mg/L (1.0 g/L) |
| L-Glutamine | 100 mg/L |
| Sodium Pyruvate | Not present |
| Phenol Red | Present (21.24 mg/L) |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | less than 0.05 EU/mL |
| Sterility USP <71> | No growth after 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> Method 1 | Compliant |
| Particulate ≥25 µm USP <788> Method 1 | Compliant |
| Water Purity | Ultrapure Type 1, 18.2 MΩ·cm (ASTM D1193 / ISO 3696 Type I) |
| Manufacturing std. | ISO 13485:2016 ISO 13485 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, protected from light |
| Freeze-thaw | Not recommended |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO2 requirement | Bicarbonate-free and HEPES-free as supplied; user must add a buffering system (sodium bicarbonate for CO2 incubator use, or HEPES for CO2-independent culture) based on the intended application |
| Parameter | Specification |
|---|---|
| Raw material grade | Cell culture / reagent grade |
| Traceability | Full lot documentation, CoA available |
| Manufacturing QMS | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch precision manufacturing |
| Intended use | For Research Use Only (RUO) |
Full composition (mg/L)
Complete formulation with CAS numbers, reproduced from the manufacturer specification. Custom compositions available on request.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 265.000 |
| Magnesium sulfate anhydrous | 7487-88-9 | 97.690 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium acetate anhydrous | 127-09-3 | 50.000 |
| Sodium chloride | 7647-14-5 | 6800.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 122.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 50.000 |
| L-Alanine | 56-41-7 | 25.000 |
| L-Arginine | 74-23-7 | 57.870 |
| L-Aspartic acid | 56-84-8 | 30.000 |
| L-Cysteine hydrochloride monohydrate | 7048-04-6 | 260.000 |
| L-Cystine dihydrochloride | 30189-89-0 | 20.000 |
| L-Glutamic acid | 56-86-0 | 75.000 |
| L-Glutamine | 56-85-9 | 100.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 20.000 |
| L-Isoleucine | 73-32-5 | 20.000 |
| L-Leucine | 61-90-5 | 60.000 |
| L-Lysine hydrochloride | 657-27-2 | 70.000 |
| L-Methionine | 63-68-3 | 15.000 |
| L-Phenylalanine | 63-91-2 | 25.000 |
| L-Proline | 147-85-3 | 40.000 |
| L-Serine | 56-45-1 | 25.000 |
| L-Threonine | 72-19-5 | 30.000 |
| L-Tryptophan | 73-22-3 | 10.000 |
| L-Tyrosine disodium salt dihydrate | 69847-45-6 | 40.000 |
| L-Valine | 72-18-4 | 25.000 |
| Trans-4-Hydroxy-L-Proline | 51-35-4 | 10.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 0.500 |
| D-Biotin | 58-85-5 | 0.010 |
| D-Pantothenate (Hemicalcium) | 137-08-6 | 0.010 |
| Folic acid | 59-30-3 | 0.010 |
| L-Ascorbic acid sodium salt | 134-03-2 | 50.000 |
| Nicotinamide | 98-92-0 | 0.025 |
| Nicotinic acid | 59-67-6 | 0.025 |
| Pyridoxal hydrochloride | 65-22-5 | 0.025 |
| Pyridoxine hydrochloride | 58-56-0 | 0.025 |
| Riboflavin | 83-88-5 | 0.010 |
| Thiamine hydrochloride | 67-03-8 | 0.010 |
| p-Amino benzoic acid (PABA) | 150-13-0 | 0.050 |
| OTHERS | ||
| 2' Deoxyadenosine | 958-09-8 | 10.000 |
| 2' Deoxycytidine hydrochloride 2' | 960-71-4 | 10.000 |
| Deoxyguanosine | 961-07-9 | 10.000 |
| 5-Methyldeoxycytidine | 838-07-3 | 0.100 |
| Cholesterol | 57-88-5 | 0.200 |
| Cocarboxylase | 154-87-0 | 1.000 |
| Coenzyme A sodium salt | 102029-73-2 | 2.500 |
| D-Glucose | 50-99-7 | 1000.000 |
| D-Glucuronic acid sodium salt | 207569-96-4 | 3.880 |
| FAD disodium salt | 84366-81-4 | 0.106 |
| Glutathione reduced | 70-18-8 | 10.000 |
| myo-Inositol | 87-89-8 | 0.050 |
| Phenol red sodium salt | 34487-61-1 | 21.240 |
| Thymidine | 50-89-5 | 10.000 |
| Tween 80 | 9005-65-6 | 5.000 |
| Uridine-5-Triphosphate.Na | 19817-92-6 | 1.000 |
| β-NAD | 53-84-9 | 7.000 |
| β-NADP sodium salt | 1184-16-3 | 1.000 |
ISO 13485:2016 manufacturing & compliance
FluxMPS™ CMRL 1066 Medium w/o Sodium Bicarbonate: 1X Liquid is manufactured under a full ISO 13485:2016 quality management system, with final packaging, testing, and customization completed at Diagnocine Precision in Totowa, New Jersey, USA.
ISO 13485:2016 QMS
Full quality management system certification covering manufacturing, testing, and release for every production lot.
Ultrapure Type 1 Water
All media prepared with 18.2 MΩ·cm resistivity water (ASTM D1193 / ISO 3696 Type I), with controlled trace-metal and TOC levels.
ISO Class 5 Fill & Finish
Aseptic filling performed in validated laminar-flow (ISO Class 5 / Class 100) workstations; 21 CFR Part 820 (QMSR) aligned.
Micro-Batch Precision
Small-batch manufacturing supports lot-to-lot nutrient consistency for reproducible perfusion studies and long-term OoC experiments.
Endotoxin — USP <85> BET
LAL assay performed per batch. Release specification: less than 0.05 EU/mL.
Particulate — USP <788> Method 1
Light obscuration particle count test confirms ≥10 µm and ≥25 µm particulate compliance on every lot.
Osmolality — USP <785>
Freezing-point osmometry performed per USP <785>. Result: Contact for specification.
Documentation — CoA & Full Lot Records
Certificate of Analysis available for every lot, including full QC panel, raw material traceability, and release signatures.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification less than 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-CMRL-B1X (FluxMPS™) compares
Side-by-side comparison against conventional 0.22 µm-filtered alternatives of the same base formulation.
| Parameter | DCP-CMRL-B1X (FluxMPS™) | Conventional CMRL 1066 (0.22 µm) | Standard CMRL 1066 alternative |
|---|---|---|---|
| Grade | Microfluidics Suitable | Standard grade | Standard grade |
| Base Formulation | CMRL 1066 w/o Sodium Bicarbonate: 1X Liquid | CMRL 1066 Standard | CMRL 1066 Equivalent |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 stages | 1 stage | 1–2 stages |
| Mycoplasma barrier filtration | check_circle | cancel | cancel |
| Endotoxin (release specification) | less than 0.05 EU/mL | Corning classical liquid media — less than 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP particulate compliance | check_circle USP <788> Method 1 | cancel | cancel |
| Water quality | Ultrapure Type 1 (18.2 MΩ·cm) | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | Variable | Variable |
| Microfluidic channel compatibility | check_circle Validated | cancel Risk of clogging | cancel Risk of clogging |
| Custom formulation | check_circle On request | cancel | Limited |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ CMRL 1066 Medium w/o Sodium Bicarbonate: 1X Liquid and Microfluidics Suitable cell culture media.
Supporting literature
Peer-reviewed publications supporting the scientific rationale for ultra-filtered media and microfluidic cell culture applications.
- Huh D et al. (2010). Reconstituting organ-level lung functions on a chip. Science, 328(5986), 1662–1668. doi:10.1126/science.1188302
- Bhatia SN & Ingber DE (2014). Microfluidic organs-on-chips. Nature Biotechnology, 32(8), 760–772. doi:10.1038/nbt.2989
- Bhattacharya S et al. (2018). Challenges in maintaining cell viability during microfluidic experiments. Electrophoresis, 39(7), 997–1006. doi:10.1002/elps.201700375
- Warburg O (1956). On the origin of cancer cells. Science, 123(3191), 309–314. doi:10.1126/science.123.3191.309
- Emmons EV (1965). Detection of mycoplasma in cell cultures using filtration. Proceedings of the Society for Experimental Biology, 118, 1010–1015. doi:10.3181/00379727-118-29988
- Kim S et al. (2012). Gut-on-a-chip microdevice replicates key functional features of the human intestine. Lab on a Chip, 12(12), 2165–2174. doi:10.1039/c2lc40074j
- Zhang YS et al. (2017). Multisensor-integrated organs-on-chips platform for automated and continual in situ monitoring of organoid behaviors. PNAS, 114(12), E2293–E2302. doi:10.1073/pnas.1612906114
- Vernetti L et al. (2017). Functional coupling of human microphysiology systems. Scientific Reports, 7, 42296. doi:10.1038/srep42296
- Schuster B et al. (2020). Automated microfluidic platform for dynamic and combinatorial drug screening of tumor organoids. Nature Communications, 11, 5271. doi:10.1038/s41467-020-19058-4
- Zheng F et al. (2021). Organ-on-a-chip systems: microengineering to biomimic living systems. Small, 17(7), 2004175. doi:10.1002/smll.202004175
