Buffered Ethanol Fixative 70%
Cat No.: DCP-BE70X
Size: 500 mL
Sterile, Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description:
Buffered Ethanol 70% (BE70) is a next-generation, alcohol-based fixative engineered to deliver exceptional histomorphologic preservation while maintaining unparalleled biomolecular integrity. Designed as a safer, non-crosslinking alternative to formalin, BE70 combines the rapid penetration of ethanol with a proprietary buffering system to minimize tissue distortion and maximize compatibility with modern diagnostic and research workflows. Ideal for laboratories prioritizing both histologic detail and downstream molecular applications, BE70 bridges the gap between traditional fixation and cutting-edge precision medicine.
Applications:
- Routine histology and cytology processing
- Biobanking for genomic/proteomic research
- Molecular pathology workflows (NGS, qPCR, FISH)
- Immunohistochemistry (IHC) and multiplex staining
- Forensic sample preservation
- Longitudinal research studies requiring archived tissue analysis
Advantages:
- Optimized Tissue Preservation: Proprietary 70% ethanol formulation buffered with PBS and glycerol ensures minimal shrinkage while retaining crisp nuclear and cytoplasmic detail.
- Enhanced Biomolecular Integrity: Non-crosslinking action preserves high-quality DNA, RNA, and proteins for PCR, sequencing, mass spectrometry, and other molecular techniques.
- Formalin-Free Safety: Eliminates formaldehyde exposure risks and IARC Class 1 carcinogen concerns.
- Superior RNA Recovery: Delivers 4x higher RNA integrity compared to formalin-fixed samples (PERM >120 vs. 32 for NBF).
- Broad Compatibility: Validated for immunohistochemistry (IHC), special stains, flow cytometry, and automated tissue processors.
- Ready-to-Use: Pre-mixed at pH 6.1 for immediate use without precipitation risks.
Composition:
| Ingredients |
Concentration |
| Ethanol |
70% |
| Glycerol |
1% |
| Glacial acetic acid |
0.5% |
| 0.5M PBS |
28.5% |
Usage Instructions:
For optimal results:
- For immersion fixation, specimens should ideally be no larger than 1mm³ to ensure complete penetration
- Fixation at room temperature for 1 hour is recommended as a starting point
- After fixation, wash specimens 3 × 5 minutes in fixation buffer
- Fixed specimens can be stored at 4°C for up to one week before subsequent processing
Safety Information:
- Flammable: Store away from ignition sources; ground containers during transfer
- Personal Protection: Wear nitrile gloves and eye protection; use in well-ventilated areas
- Spill Management: Absorb with inert material and dispose per local regulations
- Disposal: Comply with EPA guidelines for ethanol-containing solutions
Quality Control:
- Appearance: Clear solution
- pH: 6.1
- Sterility: Filtered 0.1 micron Twice and 0.04 micron once.
- DNase activity: None was detected after incubation of plasmid DNA, and this product was used for 18 hours at room temperature.
- RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
- Store at 4°C away from bright light.
- Shelf life is 24 months.
- Use before expiry date given on the product label.
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
