Blood DNA Maxi Kit (10 Preps)
Cat. No.: BMG-GD2314-01
Size: 10 Preps
Description:
A Blood DNA Maxi Kit is a specialized laboratory tool designed for large-scale extraction and purification of genomic DNA from blood samples.
Application:
- Large-scale genetic studies, including population genetics and biobanking projects.
- Molecular biology techniques such as PCR, Southern blotting, and next-generation sequencing.
- Forensic analysis for DNA extraction from crime scene samples.
- Clinical diagnostics, including genetic disease screening and cancer research.
- Veterinary and agricultural research, particularly in animal breeding and conservation genetics.
- These kits are designed to process large volumes of blood, typically ranging from 3 ml to 10 ml of whole blood.
- Blood DNA Maxi Kits can typically extract 300-600 µg of DNA from a 10 ml blood sample.
- The entire procedure can usually be completed in about 1 hour.
- In addition to whole blood, these kits can often handle other sample types such as plasma, serum, buffy coat, and body fluids.
- The purified DNA is free from contaminants and enzyme inhibitors, making it suitable for various downstream applications.
- The kits eliminate the need for phenol/chloroform extractions and time-consuming alcohol precipitation steps.
- Most kits use silica membrane or column-based purification methods.
- The kits can process blood samples treated with common anticoagulants like citrate, EDTA, and heparin.
- The extracted DNA is compatible with PCR, Southern blotting, restriction enzyme digestion, and other molecular biology techniques.
- These kits often use protease or proteinase K to reduce hemoglobin contamination and improve DNA purity.
- Lysis buffers
- Wash buffers
- Elution buffer
- Protease or proteinase K
- Spin columns or magnetic particles
- Collection tubes
Ghatak S, Muthukumaran RB, Nachimuthu SK. A simple method of genomic DNA extraction from human samples for PCR-RFLP analysis. J Biomol Tech. 2013 Dec;24(4):224-31. doi: 10.7171/jbt.13-2404-001. PMID: 24294115; PMCID: PMC3792701.
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