Anti-Varicella Zoster Virus(VZV) IE62, Mouse-Mono (62B)

Cat.No: FNK-65-353

Size: 100 μg

Storage: -20℃

Concentration: 1.0 mg/ml

Buffer: PBS- with 50% glycerol

Purity: Purified IgG fraction with protein A from hybridoma cell culture medium. 

Immunogen: Varicella-zoster virus Oka strain (vaccine strain)

Isotype: Mouse IgG1k

Reactivity: IE62 of VZV
 

Application

1. Western blotting (1/2,000~1/5,000 dilution)

2.  Immunoprecipitation (1/100)

3. Immunofluorescent and Immunocytochemical (1/50~1/100 dilution)

Not tested for other applications.
 

Background

Varicella Zoster Virus (VZV) is one of eight herpesviruses known to infect humans and vertebrates. VZV only affects humans, and commonly causes chickenpox in children, teens and young adults and herpes zoster (shingles) in adults and rarely in children. VZV is known by many names, including chickenpox virus, varicella virus, zoster virus, and human herpesvirus type 3 (HHV-3). VZV infects the nerves and causes a wide variety of symptoms. After the primary infection (chickenpox), the virus goes dormant in the nerves, including the cranial nerve ganglia, dorsal root ganglia, and autonomic ganglia. Many years after the patient has recovered from chickenpox, VZV can reactivate to cause a number of neurologic conditions.

Immediate early protein 62 (IE62) of is major transcriptional transactivator of VZV. It may interact with and recruit specific components of the general transcription machinery to viral promoters and stabilize their formation for transcription initiation. It interacts with IE4 and IE63, and with host USF and Sp1.It negatively regulates its own transcription. This immediate early protein may be necessary in virion for viral pathogenesis. IE62 consists of 1,310 amino acids with 140 kDa mass. It is phosphorylated by ORF66 protein kinase on Ser-686 and Ser-722. It is also phosphorylated by ORF47 protein kinase and human CSNK2A1/CKII.SK

Data Link

UniProt P09310 (ICP4_VZVD)


Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. NOT FOR MILITARY USE.

Data Images

65-353 Anti-Varicella Zoster Virus (VZV) IE62 antibody, mouse monoclonal (62B)



Fig.1. Identification of IE62 protein in VZV-infected cells by western blotting using anti-VZV IE62 antibody (clone 62B).

Lane 1; VZV strain pOka infected MRC-5 cell lysate

Lane 2; MRC-5 cell lysate ( uninfected negative control )

The anti-VZV IE62 antibody was used at 1/5,000 dilution.



Fig.2. Immunofluorescence staining of VZV IE62 protein in VZV-infected MRC-5 cells

VZV-infected MRC-5 cells were collected at 3 days postinfection. The cells were fixed in cold acetone and incubated at 37 °C for 1 h with anti-VZV IE62 antibody at 1/100 dilution. After washing with PBS for 10 min, Alexa Fluor 488 donkey anti-mouse IgG [H+L] (Life Technology No. A21202) was used at 1/200 dilution as the second antibody.

Nuclei were stained with Hoechst 33342. Signals were detected by confocal microscopy.
 

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