:Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer
:PBS with 0.02% sodium azide,100 μg/ml BSA and 50% glycerol.
Application
Applications
Species
Dilution
Positive Tissue
WB
Mouse, Rat
1: 200-1: 500
testis, ovary
Description
The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody recognize human TBP with MW 40 kDa and mouse/rat Tbp with MW 34 kDa.
Western blot analysis of TBP (GB113810) at dilution of 1: 500