:KLH conjugated Synthetic peptide corresponding to Mouse GAPDH
Isotype
:IgG
Purity
:Affinity purification
Subcellular location
:Cytoplasm, Nucleus, Membrane
Predicted MW.
:36 kDa
Observed MW.
:36 kDa
Uniprot ID
:P16858
Storage
:Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer
:PBS with 0.02% sodium azide,100 μg/ml BSA and 50% glycerol.
Application
Applications
Species
Dilution
Positive Tissue
WB
Human, Mouse, Rat
1: 1000-1: 2000
brain, spleen, kidney, liver
IHC/IF
Human, Mouse, Rat
1: 500-1: 1000
kidney, testis
Description
Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break downglucose for energy and carbon molecules. has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis, ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport. In sperm, a testis-specific isoenzyme GAPDHS is expressed.
Western blot analysis of GAPDH (GB11002) at dilution of 1: 1000
Western blot analysis of GAPDH (GB11002) at dilution of 1: 1000
Immunohistochemistry of paraffin embedded mouse kidney using GAPDH (GB11002) at dilution of 1: 500
Immunohistochemistry of paraffin embedded rat testis using GAPDH (GB11002) at dilution of 1: 500