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Anti-SUMO-1 antibody, rat monoclonal (4D12), Biotin-conjugated

Product#: FNK-70-654

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Anti-SUMO-1 antibody, rat monoclonal (4D12), Biotin-conjugated

Product ID and Size

Cat. No.	Size	Labeled	Data Link	Link
FNK-70-653	100 µg	Unlabeled	Swiss-Prot P 63165
FNK-70-654	50 µg	Biotinylated	Swiss-Prot P 63165
FNK-70-655	50 µg	FITC-conjugated	Swiss-Prot P 63165

General information

Cat. No.	：70-654
Size	：50 µg
Label	：Biotin
Antigen Species	：Human
Host Species	：Rat
Specificity	：Specific to human, simian, mouse and rat SUMO1. Other species have not been tested.
Immunogen	：Recombinant GST-fused human SUMO-1 (full length)
Product	：The antibody was produced in serum-free medium and purified by proprietary chromatography procedures under mild conditions and conjugated with biotin.
Isotype	：Rat IgG 2a kappa
Form	：1mg/ml in PBS, 50% glycerol, filter-sterilized. Azide- and carrier protein-free.
Application	1. Western blotting (1/1,000 dilution) 2. Immunofluorescence staining (1/100 dilution) 3. Immunohistochemistry, frozen section (1/100 dilution) 4. ELISA (assay dependent) Other applications have not been tested.
Cross Reactivity	：Human/Mouse/Rat
Storage	：Shipped at 4℃ or -20℃ and store at -20℃. Do not freeze.
Data Link	：Swiss-Prot P63165 (human)

Description

SUMO (Small Ubiquitin-like Modifier) proteins are a family of small proteins that are covalently attached to and detached from other proteins in cells to modify their function. Unlike ubiquitination which targets proteins for degradation SUMO modification plays a critical role in a number of cellular functions including nucleocytoplasmic transport gene expression cell cycle and formation of subnuclear structures such as promyelocytic leukemia (PML) bodies.

There are three confirmed SUMO isoforms in human; SUMO1 SUMO2 and SUMO3. SUMO2 /3 show a high degree of similarity to each other and are distinct from SUMO-1. Individual SUMO family members are all targeted to different proteins with diverse biological functions. SUMO-1 is conjugated to RanGAP PML p53 and IκB-α to regulate nuclear trafficking formation of subnuclear structures regulation of transcriptional activity and protein stability. SUMO1 is encoded as a 101 aa protein and first Met and C-terminal 4 aa are removed from the preprotein.

References: This antibody was used in Ref. 3 and 4.

1. Ulrich HD "The fast-growing business of SUMO chains.” Review Mol Cell 32: 301–305 (2008) PMID: 18995828
2. Cheng J et al “Role of desumoylation in the development of prostate cancer.” Review Neoplasia 8: 667-676 (2006) PMID: 16925949
3. Uchimura Y et al “Involvement of SUMO modification in MBD1- and MCAF1-mediated heterochromatin formation.” J Biol Chem 281: 23180-23190 (2006) PMID: 16757475
4. Saitoh N et al “In situ SUMOylation analysis reveals a modulatory role of RanBP2 in the nuclear rim and PML bodies.” Exp Cell Res 312: 1418-1430 (2006) PMID: 16688858

Figures

		Fig.1 Detection of SUMO-1 by Western blotting with the antibody 4D12. An 80 kDa single and other multiple bands were observed in HeLa total cell extract. The 80 kDa band would be SUMO-RanGAP. Anti-SUMO-1 antibody 4D12 was used at 1 μg/ml.
		Fig.2 Immunofluorescence staining of SUMO-1 with the antibody 4D12 in the mouse primary culture neurons. Left: Stained with anti-SUMO-1 antibody 4D12 at 10 μg/ml. Right: DNA was stained with Hoechst
		Fig.3 SUMO-1 colocalizes with SUMO2/3 as revealed by indirect immunofluorescence staing of C-33A cells (human cervix carcinoma). Left: SUMO-1 was stained withanti-SUMO1 antibody (4D12) at 10 μg/ml. Middle: SUMO2/3 was stained with anti-SUMO2/3 antibody (3H12) at 10 μg/ml. Right: Merged image
		Fig.4 Fluorescence immunocytochemistry for endogenous SUMO1 expression. HEK293A cells were fixed, permeabilized, and stained with SUMO1-FITC (1: 50) antibody and Hoechst 33342. Scale bar, 50 mm.