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Anti-SLA (haploid sperm cell-specific antigen) antibody, (TRA54), 50 ug

Product#: FNK-73-001

$346.79

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Anti-SLA (haploid sperm cell-specific antigen) antibody, (TRA54), 50 ug

DiagnoCine offers excellent antibody for SLA (TRA54); UNCONJUGATED large size or a small size to researchers studying Cd3zeta Deficiency, EPH-Ephrin signaling, PDGFR-beta signaling, and genes with SH3/SH2 adaptor activity.

SLA Adapter protein negatively regulates T-cell receptor (TCR) signaling, inhibits T-cell antigen-receptor induced activation of nuclear factor of activated T-cells, and involved in the mitosis of T-cells. SLA activities are linked to ZAP70 with CBL, leading to a CBL dependent degradation of signaling proteins.

SLA antibody has excellent quality and this highly pure antibody can be adapted for Western Blots, ELISA, Immunohistochemistry, Immunofluorescence research with optimization.

General Information

Cat. No.	：FNK-73-001
Size	：50 µg
Type	：Monoclonal
Host Species	：Rat
Cross Reactivity	：Mouse and rat. Not tested for other species
Label	：Unlabeled
Clone No	：TRA54
Product	：The antibody was purified from the serum-free culture medium of the hybridoma clone TRA54 by proprietary chromatography processes under mild conditions.
Application	： Western blotting (~1 ug/ml) (Ref. 1, 2) Immunohistochemical staining (Ref. 1,2 ,3) Immuno-electron microscopy (Ref. 3). Not tested in other applications
Form	：Purified rat IgM (1 mg/ml) in PBS(-), 50% glycerol
Storage	：Store at -20℃

Description

Many transcripts for proteins specifically expressed in haploid germ cells are synthesized in the spermatogenesis. A monoclonal antibody TRA54 recognizes specific organelles in germ cell cytoplasm from spermatocytes to spermatids (1, 2). Western blots using TRA54 revealed broad bands with 85 kD, 190 kD and >200 kDa in testis probably due to alternative splicing and/or post-translational modification (Fig. 1).

The molecular size diversity is due to the difference in glycosilation (Ref. 1). This antibody can be used for immunohistochemical staining of testicular tissues (1, 3). The hybridoma producing TRA54 was obtained from the immunaization of a rat with cell lysates of adult mouse testes by the group of Prof. Yoshitake Nishimune of Osaka University.

Fig.1. Western blot analysis of various mouse
tissues with TRA54 antibody.
For each tissue homogenate, 100 ug protein was
applied to each lane. Antibody was used at 1 ug/ml

Fig. 2. Immunohistochemical staining of mouse testicular cross-section with TRA54 antibody.
Testis from C57BL/6 adult mouse was fixed in Bouin’s solution, followed by paraffin embedding and sectioning. After deparaffinized, sections were treated with normal goat serum, reacted with TRA54 antibody and incubated with biotinylated sheep anti-rat Ig antibody and biotin peroxydase/avidin, followed by incubation with hydrogen peroxide and diaminobenzidine.

References (Antibody TRA54 has been used in the following publications.)

Pereira LA et al “Characterization and expression of a stage specific antigen by monoclonal antibody TRA 54 in testicular germ cell” Int J Androl 21: 34-40 (1998) PMID: 9639150　WB, IHC
Arrotéia KF et al “Identification and characterization of an antigen recognized by monoclonal antibody TRA 54 in mouse epididymis and vas deferens”J Androl 25: 914-921 (2004) PMID: 15477363　WB, IHC
Ventelä S et al ”Intercellular organelle traffic through cytoplasmic bridges in early spermatids of the rat: mechanisms of haploid gene product sharing” Mol Biol Cell 14: 2768-2780 (2003) PMID: 12857863 IHC, Immno-Electron Microscopy

Aliases for SLA Gene