Anti-Nestin antibody. rat monoclonal(7A3)
Anti-Nestin antibody, rat monoclonal (7A3)
DiagnoCine offers excellent Nestin I Intermediate Filament Protein Antibody to researchers studying central/Peripheral nervous systems, a biomarker for stem cells, hippocampal neurogenesis, intratumor heterogeneity, cognitive dysfunction, human neurogenesis, neural stem cells, bone marrow endothelial cells, and stromal heterogeneity.
Human diseases include Alzheimer's Disease, pancreatic ductal adenocarcinoma, breast cancer, squamous cell carcinoma, dementia, glioblastoma multiforme (GBM), major depressive disorder, and HIV-1 associated neurocognitive disorder.
Anti-Nestin I Intermediate Filament Protein Antibody has excellent quality and this highly pure antibody can be adapted for western blotting, crystal violet staining, and other methods of detection with optimization.
General information
| Cat. No. | ：FNK-73-100 |
| Size | ：200 ug |
| Host Species | ：Rat |
| label | ：Unlabeled |
| Clone | ：7A3 |
| Isotype | ：Rat IgG2b κ |
| Immunogen | ：Mouse E16 embryonic cerebral cortex extracts |
| Specificity | ：Specific to mouse Nestin, not tested with other species. |
| Form | ：Purified monoclonal antibody (IgG) 1mg/ml in PBS, 50% glycerol, filter-sterilized |
| Application | ：1. Immunocytochemistry ：2. Immunohistochemistry ：This antibody doesn’t work in immunoblotting. Other applications are not tested. This antibody is very useful for immunostaining of mouse embryonic brain because it is rat antibody. Rat antibody has very low background in immunostaining using mouse tissues and is also useful for double-staining with mouse and rabbit antibodies. |
| Storage | ：Shipped at 4℃ or -20℃, and stored at -20℃. |
| Data Link |
：UniProtKB/Swiss-Prot Q6P5H2 (NEST_MOUSE) |
Description
Nestin is an intermediate filament protein that is expressed in stem cells and progenitor cells in the mammalian central nervous system (CNS) during development. Nestin is replaced in the adult organism by other intermediate filament proteins, however, it may be re-expressed under certain pathological conditions such as ischemia, inflammation, brain injury, and neoplastic transformation. Nestin has been detected in many kinds of tumors, especially in tumors derived from the CNS, therefore it is considered to be a marker for cancer stem cells in neurogenic tumors. The antibody was produced from the hybridoma cultured in serum-free medium and purified under mild conditions by propriety chromatography processes.
Fig.1 Primary culture of neural progenitor cells from mouse fetal brain stained with 7A3 (Left), stained with Hoechst (Center), and without staing (Right).
References
- Hockfield S & McKay RD "Identification of major cell classes in the developing mammalian nervous system" J Neurosci 5: 3310-3328 (1985) PMID: 4078630
- Gilyarov AV “Nestin in central nervous system cells” Neuroscience and Behavioral Physiology 38:165-169 (2008) PMID: 18197384
Aliases for NES Gene
