Anti-Laminin 511, Human, Mouse-Mono (12D)

Product#: FDV-0026
$400.00
Availability:
Ships in 1-2 weeks

Anti-Laminin 511, Human, Mouse-Mono (12D)


General information 

Cat. No. FDV-0026
Size 100 ul
Format Mouse ascites
Formulation Ascites without any additives
Host Species and Clonality Mouse monoclonal
Isotype and Subclass  IgG2a
Purification No purification
Lot Number see vial label
Specificity Human, other species not tested yet
Application  
  1. Western blotting under both reducing, and non-reducing conditions (1/1,000-1/10,000)
  2. Immunohistochemistry with paraffin and frozen sections
  3. Immunoprecipitaion and immunoaffinity purification
  4. ELISA
  5. NOTE: The reactivity on reducing conditions of western blotting is much weaker than on non-reducing conditions.
Storage For short-term storage, -20oC. For long-term storage, -80oC storage is preferable. 
    Avoid repeated freeze-thaw cycles and avoid storage at 4oC

Description

Useful monoclonal antibodies for the research of cancer pathology 4 unique monoclonal antibodies against cancer-related laminin chains

Laminins are major extracellular matrix proteins containing over 15 members. Laminins are large hetero-trimers consisting of 5 alpha chains, 3 beta chains, and 3 gamma chains. Due to its structural varieties, it has been challenging to detect each laminin isoform by conventional antibodies.

Funakoshi commercializes 4 unique antibodies, originally developed by Dr. Kaoru Miyazaki, Professor Emeritus of Yokohama City University in Japan. 
Our antibodies can separately recognize specific isoforms or structures of laminin.
FDV-0023_fig1.png
Fig.1 Structure of Laminin 3A32, 3B32 and γ2pf fragment with epitopes of 3 clones (F7, BG5 and P2H).

Features
  • BG5/F7 : For laminin alpha-3 chains
    • Laminin alpha 3 chain, a major component of Laminin-332, has two splicing variants, alpha 3A and alpha 3B
    • Clone BG5 is specific antibody for alpha 3A
    • Clone F7 is the world first commercially available antibody for alpha 3B
  • P2H : For laminin gamma-2 chain
    • Laminin gamma2, an only component of Laminin-332, is a marker gene of invasive cancer tissue
    • Laminin gamma-2 chain is activated via proteolytical cleavage at N-terminal
    • N-terminal proteolytic fragment of gamma-2, called γ2pf, is highly accumulated into cytosol under malignant cancer
    • Clone P2H is the world first antibody for γ2pf
  • 12D : For laminin-511
    • There are two similar isoforms, laminin-511 and laminin-521 and it is difficult for conventional antibodies such as anti-alpha 5 chain to separate them.
    • Clone 12D specifically react with trimeric structure of laminin-511, not react with each components alpha 5, beta 1, gamma 1 and similar isoform laminin-511.
Note
Laminin 511 is used as a culture substrate for ES and iPS cells. (See our iMatrix-511 / Easy iMatrix-511).
FDV-0023_fig2.png
Fig.2 Structure of Laminin-511
 

Clone

BG5

F7

P2H

12D

Target

Laminin-α3A chain

Laminin-α3B chain

Laminin-γ2 chain N-terminal region

Laminin-511 trimer

Normal Tissue

FDV-0023_1-1.jpg

FDV-0023_1-2.jpg

FDV-0023_1-3.jpg

FDV-0023_1-4.jpg

Epitope

FDV-0023_2-1.jpg

FDV-0023_2-2.jpg

FDV-0023_2-3.jpg

FDV-0023_2-4.jpg

Cancer Tissue

FDV-0023_3-1.jpg
 

FDV-0023_3-2.jpg
 

FDV-0023_3-3.jpg
 

FDV-0023_3-4.jpg
 


Outline of four clones
 

Description

Anti-Laminin alpha 3A

Anti-Laminin alpha 3B

Anti-Laminin gamma 2 N-terminal Fragment

Anti-Laminin-511

Clone

BG5

F7

P2H

12D

Specificity

Laminin alpha 3A*1

Laminin alpha 3B

Laminin gamma 2 Domain V-NE2

Laminin-511

Host and Type

Mouse Monoclonal

Subclass

IgG2a

IgG1

IgG2a

IgG2b

Reactivity

Human

Human

Human and Mouse

Human

Form

Mouse Ascites (Unpurifed)

     

Application

ELISA, IHC, IP, WB

IHC (frozen & paraffin sections), WB

IHC (frozen), IP, WB Non-reducing) *2

Code

FDV-0024

FDV-0023

FDV-0025

FDV-0026

Size

100 uL

Storage

-20 *3


*1 Clone BG5 only detects alpha 3A in IHC. However, this clone may react with alpha 3B in ELISA, IP and Western Blot.
*2 Clone 12D can be only used for IHC (Frozen) and under non-reducing condition for Western Blot.
*3 Non-preservative added. So these 4 clones can be degraded at 4C. We recommend -20C storage.

Data Examples

FDV-0023_fig3.png
Fig.3 IHC image of skin cancer tissue in clone BG5 and F7
Left : Clone BG5, Right : Clone F7
Both clones detected basal membrane like structure around the tumor but F7 clone also detected vascular basement membrane (arrow heads)

FDV-0023_fig4.png
Fig.4 IHC image of tissues in clone P2H
Upper images : Normal mammary gland
Lower images : Breast cancer
P2H stains basement membranes surrouding mammary glands in normal tisue, whereas in a breast cancer tissue cytoplasmic accumulation of γ2pf orits fragments is detected.

FDV-0023_fig5.png
Fig.5 IHC image of breast cancer tissue in clone 12D
A : Vascular basement membrane
B : Mammary gland basement membrane
C : Basement membrane-like structures surrounding tumor cells

FDV-0023_fig6.png 
Fig.6 Specificity of clone 12D against laminin isoforms
Seven recombinant human laminins were coated on a multi-well plate and detected by clone 12D. The data shows clone 12D specifically reacts with Laminin 511.

Reference
  1. Kariya et al., J. Biol. Chem., 279, 24774-24784 (2004) Characterization of laminin-5B (a3Bß3r2) and NH2- terminal proteolytic fragment of its a3B chain: Promotion of cellular adhesion, migration and proliferation.
  2. Kariya et al., J. Mol. Histol., 39, 435-446 (2008) Localization of laminin alpha3B chain in vascular and epithelial basement membranes of normal human tissues and its down-regulation in skin cancers.
  3. Mori et al., J. Biol. Chem., 285, 35068-35078 (2010) Laminin-3B11, a novel vascular type laminin capable of inducing prominent lamellipodial protrusions in microvascular endothelial cells.
  4. Mori et al, Camcer Sci., 102, 1095-1100 (2011) Downregulation of a newly identified laminin, laminin-3B11, in vascular basement membranes of invasive human breast cancers.
  5. Miyazaki et al., Cancer Sci., 107, 1909-1918 (2016) Highly sensitive detection of invasive lung cancer cells by novel antibody against amino-terminal domain of laminin gamma2 chain.
 
Logo_Funakoshi.png

Satisfaction
Quality Rating
Value Rating
Style Rating
X