Anti-Cry j1, Mouse-Mono(053)
Cat. No.: FNK-HBL-Ab-1-053
Size: 100 µg, 200 µl/vial (Frozen Liquid)
Storage: -20°C. Avoid freeze-thaw cycles by storage in appropriate aliquots.
Host Species: Mouse
Class: IgG
Purification: Protein A/G Affinity Purified
Specifications
This antibody recognizes Cry j 1 antigen (Japanese cedar pollen allergen). This antibody cross-reacts against Cry j 2 antigen less than 0.1%.Isotype:
Mouse monoclonal antibody IgG1,κ
Preparation & Form:
The hybridoma was inoculated in mice intraperitoneally. IgG was purified from collected ascites by ProteinG Sepharose column chromatography.
Purified IgG was prepared in the concentration of 500μg/ml with phosphate-buffered saline and frozen. No stabilizers or preservatives are contained.
Purity:
Greater than 95%. Cry j 1 antigen is contained less than 0.1%, measured with ELISA.
References:
1) Kawashima, T. et al.:Antigenic analysis of Sugi basic protein by monoclonal antibodies:I. Distribution and characterization of B-cell-tropic epitopes of Cry j I molecules. Int. Arch. Allergy Immunol., 98(2), 110-117, 1992
2) Kawashima, T. et al.:Antigenic analysis of Sugi basic protein by monoclonal antibodies:II. Detection of immunoreactive fragments in enzyme-cleaved Cry j I. Int. Arch. Allergy Immunol., 98(2), 118-126, 1992
3) Sawatani, M et al. : Immunological and physiological properties of Cry j II, the second major allergen of Japanese cedar pollen. [Article in Japanese] Arerugi, 42(6):738-747, 1993
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