0
Precision Quality to Your Research verified ISO 13485 Certified | SBA WOSB | NJ MWBE · NYC MWBE · Illinois BEP arrow_forward View Details

FluxMPS™, PVP Protein Extraction Buffer

Product#: DCP-PVPPC1X
$175.71
Availability:
Ships in 24 hours

ISO 13485 Certified Manufacturing

FluxMPS™ PVP Protein Extraction Buffer

An MPS-grade, Tris-based protein extraction system built for polyphenol- and pigment-rich plant tissues. Solution A pairs 1% PVP-40 with 5 mM TCEP-HCl for stable, thiol-protected protein isolation, and is supplied with a separate 100X protease inhibitor cocktail (Solution B) for flexible, protocol-specific use.

  • Two-component kit: Solution A extraction buffer (100 mL) plus Solution B, a 100X protease inhibitor cocktail (1 mL).
  • PVP-fortified formulation (1% PVP-40) binds polyphenols and pigments to reduce protein crosslinking and assay interference in difficult plant tissues.
  • TCEP-HCl (5 mM) provides a stable, thiol-free reducing environment that protects cysteine residues and limits oxidative protein damage.
  • Tris (50 mM) and sodium chloride (150 mM) create a physiological-like base that supports protein solubility and native structure.
  • Single-stage 0.1 µm filtration for clean, low-particulate reagent quality.
  • Ultrapure Type 1 water (18.2 MΩ·cm).
  • Compatible with SDS-PAGE, western blotting, enzyme assays, and total protein quantification.
  • Customizable formulation available upon request.
SKU: DCP-PVPPC1X | UNSPSC: 12161703 Plant Biology Buffers
FluxMPS™ PVP Protein Extraction Buffer Kit
  • pH7.5 (Solution A)
  • AppearanceClear solution
  • Filtration0.1 µm
  • FormatSolution A (100 mL) + Solution B (1 mL, 100X)
  • Dilution RatioSolution B added to Solution A at 1:100
  • Storage (Solution A)2-8 °C, avoid repeated freeze-thaw
  • Storage (Solution B)-80 °C long term, avoid repeated freeze-thaw
  • Shelf Life1 year
  • Manufacturing StandardISO 13485:2016
  • Intended UseResearch Use Only (RUO)
ISO 13485:2016 RUO
This product is supplied as a two-component kit: Solution A, the PVP/TCEP extraction buffer base (100 mL), and Solution B, a 100X protease inhibitor cocktail (1 mL), packaged and shipped together.
Why FluxMPS™

Engineered where standard extraction buffers fail

Conventional, single-purpose extraction buffers leave polyphenols, pigments, and oxidized cysteines to interfere with downstream protein analysis. PVP Protein Extraction Buffer combines a polyphenol-binding base, a stable reducing agent, and single-stage 0.1 µm filtration in one ISO 13485-manufactured kit.

filter_alt

Microchannel-safe purity

Single-stage 0.1 µm filtration removes large particulates, aggregates, and bacteria, providing baseline purity beyond standard 0.22 µm-filtered reagents.[6]

target

Physiological Tris/NaCl base at pH 7.5

50 mM Tris and 150 mM NaCl support protein solubility and native structure; Solution A is formulated to pH 7.5.

water_drop

Ultrapure-grade water

Manufactured with ultrapure Type 1 water (18.2 MΩ·cm) to minimize background contamination.

visibility

Broad downstream compatibility

Compatible with SDS-PAGE, western blotting, enzyme assays, and total protein quantification.

science

PVP-40 phenol protection and TCEP-HCl reduction

1% PVP-40 complexes polyphenols and pigments that cause protein crosslinking[4], while 5 mM TCEP-HCl maintains cysteine residues in a reduced state and limits oxidative damage during extraction.[5]

tune

Customization on demand

pH, molarity, salt composition, and additive content can be adjusted on request for protocol-specific extraction needs.

Purity Architecture

Single-stage 0.1 µm filtration

Solution A is manufactured with a single 0.1 µm membrane filtration pass, exceeding the particulate control of standard 0.22 µm-filtered laboratory reagents.[6]

  1. 1

    0.1 µm filtration

    Removes large particulates, aggregates, and bacteria. Primary guard providing baseline purity exceeding standard 0.22 µm filtration.

Performance vs. conventional buffer

Standard 0.22 µm single-pass filtration leaves particles in the 0.12-0.22 µm range. The 0.1 µm final filter used here is 2.2 times finer than a 0.22 µm membrane, reducing the particulate load carried into downstream assays.

0.1 µm
Final filtration stage
1
Total filtration stages
Manufactured under processes aligned with USP <788> particulate-matter guidance; single-pass 0.1 µm filtration is applied to Solution A prior to fill.
Diagram of the FluxMPS single-stage 0.1 micron filtration system used for DCP-PVPPC1X PVP Protein Extraction Buffer, engineered for plant biology and organ-on-a-chip sample preparation workflows by Diagnocine
Figure 1. Single-stage 0.1 µm sequential filtration architecture used to manufacture Solution A of the PVP Protein Extraction Buffer kit. © Diagnocine® - DCP-PVPPC1X
Applications

Validated and supported applications

PVP Protein Extraction Buffer is designed for plant tissues rich in polysaccharides and polyphenols, and for other complex samples where standard guanidinium-based reagents give low yield or contaminated extracts.[1][2][9]

Automated Bioreactors & Robotics

Next-Generation System Uptime

For automated sample-preparation platforms and robotic liquid handling, an optional 0.01 µm (10 nm) ultra-filtered variant of Solution A is available to further protect valves, sensors, and fine-bore tubing from particulate carry-over.

  • Total particulate exclusion for automated fluid paths.
  • Valve and sensor protection in robotic liquid-handling systems.
  • Extended stability for continuous or repeated dispensing cycles.

Inquiry Required: the 0.01 µm ultra-filtered grade is produced to order; contact support@diagnocine.com to request this variant.

Microfluidics

Micro Physiological System (MPS) & Chip Sample Prep

Low-particulate extraction buffer suitable for feeding downstream microfluidic and chip-based protein assays.[8]

OoCToCBoCLoCMPS
Sample Preparation

Plant Tissue Lysis & Homogenization

Homogenize tissue in 5-10 volumes of cold buffer, keeping samples on ice throughout extraction.

LysisHomogenizationDilution
Plant Biology

Recalcitrant Plant Tissue Protein Extraction

Formulated for leaves, roots, seeds, and woody tissues where polyphenols and pigments interfere with standard extraction chemistries.[2]

LeavesRootsSeedsWoody Tissue
Protein Biochemistry

Native Protein Interaction Studies

Mild, non-denaturing Tris/NaCl base supports native protein interactions needed for co-immunoprecipitation.[10]

Co-IPNative PAGE
Immunoassays

SDS-PAGE, Western Blotting & Enzyme Assays

Extracted protein is compatible with SDS-PAGE, western blotting, enzyme assays, and total protein quantification.[3]

SDS-PAGEWestern BlotELISA
Cell & Culture Biology

Callus & Plant Cell Culture Extracts

Also suitable for protein extraction from callus cultures and other complex plant-derived samples (user validation recommended).

Callus CulturePlant Cell Culture
Protocol overview: equilibrate buffer and samples on ice; immediately before use, add Solution B (protease inhibitor cocktail) to Solution A at 1:100 dilution; homogenize tissue or cell pellets in 5-10 volumes of cold buffer (for example, 0.5-1 mL per 50-100 mg tissue); centrifuge at 10,000-20,000 × g for 10-20 minutes at 4 °C to remove debris; collect the supernatant as the clarified protein extract and use directly or store aliquots at -20 °C or -80 °C as appropriate.
Technical Specifications

Technical specifications

Specification values shown below reflect the components and parameters stated for this kit.

Physical & Chemical Parameters
Parameter Specification
Formulation Two-component kit: Tris/NaCl extraction buffer with PVP-40 and TCEP-HCl (Solution A) plus a 100X protease inhibitor cocktail (Solution B)
Appearance Clear solution (Solution A)
pH USP <791> 7.5 (Solution A)
Tris base concentration 50 mM (Solution A)
Sodium chloride concentration 150 mM (Solution A)
Sterility, Purity & Safety Parameters
Parameter Specification
Filtration (Solution A) 0.1 µm single-stage membrane filtration
Water quality Ultrapure Type 1 water (18.2 MΩ·cm)
Manufacturing standard ISO 13485:2016
Storage, Handling & Logistics
Parameter Specification
Storage temperature (Solution A) 2-8 °C
Storage temperature (Solution B) -80 °C (long term)
Freeze-thaw handling Avoid repeated freeze-thaw cycles (both solutions)
Shelf life 1 year (both solutions)
Raw Materials & Regulatory Traceability
Parameter Specification
Manufacturing QMS ISO 13485 Certified quality management system with full traceability and lot documentation
Production site DiagnoCine Precision, Totowa, NJ, USA
Intended use Research Use Only (RUO)
Formulation

Full composition

Component concentrations are shown exactly as formulated for each solution in this kit.

Component CAS Number Concentration
Tris base 77-86-1 50 mM
Sodium Chloride 7647-14-5 150 mM
PVP-40 9003-39-8 1%
TCEP-HCl 51805-45-9 5 mM
Component CAS Number Concentration
AEBSF·HCl 30827-99-7 10 mM
Aprotinin 9087-70-1 80 µM
Bestatin 58970-76-6 5 mM
E-64 66701-25-5 100 µM
Leupeptin 103476-89-7 1 mM
Pepstatin A 26305-03-3 0.1 mM
Custom pH, molarity, salt composition, and additive content are available on request - contact support@diagnocine.com.
Quality Assurance

Manufacturing & compliance

PVP Protein Extraction Buffer is manufactured under a certified quality management system with defined, traceable processes.

verified

ISO 13485:2016 Certified

Manufactured under certified QMS with full traceability and lot documentation.

water_drop

Ultrapure Type 1 Water

Formulated with 18.2 MΩ·cm ultrapure water to minimize background contamination.

biotech

ISO Class 5 Fill & Finish

Final fill performed in an ISO Class 5 (Class 100) controlled environment.

assignment

Micro-Batch Precision

Small, controlled batch sizes support consistent formulation and lot-to-lot documentation for both kit solutions.

Filtration Process Control

Solution A is filtered through a 0.1 µm membrane consistent with USP <788> particulate-matter guidance for controlled reagent manufacturing.

Quality Management

Manufactured, tested, and released under an ISO 13485:2016-certified quality management system.

Made in USA

All manufacturing, QA testing, and final assembly performed at DiagnoCine Precision, Totowa, NJ.

Documentation & CoA

Lot-specific documentation is maintained for both Solution A and Solution B.

Certificates of Analysis and additional QC documentation are available upon request - contact support@diagnocine.com.
Product Comparison

How DCP-PVPPC1X compares

A side-by-side look at the PVP Protein Extraction Buffer kit against conventional plant extraction reagents.

Parameter DCP-PVPPC1X (FluxMPS™) Conventional Extraction Buffer Standard RIPA-Style Buffer
PVP-40 polyphenol binding check_circle cancel cancel
TCEP-HCl thiol-free reduction check_circle cancel cancel
Final filtration pore size 0.1 µm 0.22 µm 0.22 µm
Native protein structure preservation check_circle cancel cancel
Water quality Ultrapure Type 1 (18.2 MΩ·cm) Not specified Not specified
Manufacturing QMS ISO 13485:2016 Not specified Not specified
Custom formulation Available on request Not available Not available
FAQ

Frequently asked questions

Common questions about the PVP Protein Extraction Buffer kit.

The kit is formulated primarily for bench-scale plant protein extraction. Its low-particulate, single-stage 0.1 µm filtered Solution A is compatible with feeding protein extracts into downstream microfluidic or chip-based assays.
Solution A is manufactured with a single 0.1 µm membrane filtration pass, which is 2.2 times finer than a standard 0.22 µm membrane, removing more particulates, aggregates, and bacteria before fill.
Solution A is formulated to pH 7.5 with 50 mM Tris and 150 mM NaCl. This mild, non-denaturing base (no SDS or deoxycholate) differs from RIPA-style buffers and preserves native protein structure, which also supports co-immunoprecipitation. pH, molarity, and salt composition can be adjusted on request.
Store Solution A at 2-8 °C and avoid repeated freeze-thaw cycles; equilibrate the buffer and samples on ice before use to maintain consistent extraction conditions.
Yes. Custom formulation is available upon request. Solution B, the 100X protease inhibitor cocktail, should be added to Solution A at 1:100 dilution immediately before use.
This kit is a Research Use Only reagent and is not routinely endotoxin-tested. For lot-specific QC documentation requests, contact support@diagnocine.com.
Lot-specific documentation is maintained for both Solution A and Solution B under the ISO 13485:2016-certified quality management system. Contact support@diagnocine.com to request a Certificate of Analysis.
Scientific References

Supporting literature

Citations and curated literature relevant to plant protein extraction and this buffer's formulation chemistry.

Citations

  1. Wang W, Vignani R, Scali M, Cresti M. A universal and rapid protocol for protein extraction from recalcitrant plant tissues for proteomic analysis. Electrophoresis. 2006;27(13):2782-2786. doi:10.1002/elps.200500743
  2. Saravanan RS, Rose JKC. A critical evaluation of sample extraction techniques for enhanced proteomic analysis of recalcitrant plant tissues. Proteomics. 2004;4(9):2522-2532. doi:10.1002/pmic.200300789

Supporting Literature

  1. Burnette WN. Western blotting: electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels. Anal Biochem. 1981;112(2):195-203. doi:10.1016/0003-2697(81)90281-5
  2. Loomis WD. Overcoming problems of phenolics and quinones in the isolation of plant enzymes and organelles. Methods Enzymol. 1974;31:528-544. doi:10.1016/0076-6879(74)31057-9
  3. Burns JA, Butler JC, Moran J, Whitesides GM. Selective reduction of disulfides by tris(2-carboxyethyl)phosphine. J Org Chem. 1991;56(8):2648-2650. doi:10.1021/jo00011a027
  4. Good NE, Winget GD, Winter W, Connolly TN, Izawa S, Singh RM. Hydrogen ion buffers for biological research. Biochemistry. 1966;5(2):467-477. doi:10.1021/bi00866a011
  5. Sosnowski O, et al. Sub-micron filtration strategies for particulate control in laboratory reagent manufacturing. J Pharm Sci Technol. 2015;69(4):512-520. doi:10.5731/pdajpst.2015.01059
  6. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32(8):760-772. doi:10.1038/nbt.2989
  7. Jorrin-Novo JV, Maldonado AM, Echevarria-Zomeno S, et al. Plant proteomics update (2007-2008). J Proteomics. 2009;72(3):285-314. doi:10.1016/j.jprot.2009.01.026
  8. Phizicky EM, Fields S. Protein-protein interactions: methods for detection and analysis. Microbiol Rev. 1995;59(1):94-123. doi:10.1128/mr.59.1.94-123.1995

Related Products

Recently Viewed

FluxMPS™, TCA Protein Precipitation Kit

$319.00
Satisfaction
Quality Rating
Value Rating
Style Rating

X