Anti Human Paraoxonase-1 Antibody PON-4C-1, Biotinylated
Anti Human Paraoxonase-1 Antibody PON-4C-1, Biotinylated
General information
| Cat. No. | ：FNK-BML027 |
| Size | ：50µg |
| Clone | ：PON-4C-1 |
| Antigen | ：Human |
| Host Animal | ：Mouse |
| Cross Reactivity | ：Human |
| Labeled | ：Biotin |
| Preparation | ：Produced in mice immunized with paraoxonase-1 (PON-1) purified from human plasma. PON-1 specific IgG was purified from mouse ascites fluid with a proteinA-Sepharose. |
| Formulation | ：0.2 µm filtered PBSsolution |
| Specificity | ：This antibody has been selected for its ability to bind for human paraoxonase-1 (1). |
| Application |
：Western Blot – This antibody can be used at 0.5 – 1.0 µg/mL with the appropriate secondary reagent to detect human plasma PON-1. The detection limit for purified PON-1 and plasma sample is approximately 0.01 µg/lane and 0.05 µL,respectively, under non-reducing and reducing conditions. ：Sandwich ELISA – This antibody can be used as a detection antibody in a human PON-1 ELISA in combination with the monoclonal capture antibody (Catalog #PO510D). A general protocol is provided on the next page. Using plates coated with 100 µL/well of the capture antibody, in combination with 100 µL/well of the detection antibody at 500 ng/mL, an ELISA for sample volumes of 100 µL can be obtained. Titrate each preparation of the serum sample for standard preparation to arrive at the most suitable dose range.For this antibody pair, a two-fold dilution series starting at 600 pg/mL is suggested. For more information, please see the nextpage or the reference (1). Optimal dilutions should be determined by each laboratory for each application. |
| Immunogen | ：Paraoxonase-1 purified from pooled plasma |
| Ig Type | ：IgG2b |
| Storage | ：IgG in PBS solution are stable for twelve months from the date of receipt when stored at-80˚C. Avoid repeated freeze-thaw cycles. |
References
- Kujiraoka et al., A sandwich enzyme-linked immunosorbent assay for human serum paraoxonase concentration. J Lipid Res, 2000;41:1358-1363
- van Himbergen et al., Indications that paraoxonase-1 contributes to plasma high density lipoprotein levels in familial hypercholesterolemia. J Lipid Res, 2005;46:445-451.
- Kujiraoka et al.,Effects ofintravenous apolipoproteinA-I/phosphatidylcholine discs on paraoxonase and platelet-activating factor acetylhydrolase in human plasma and tissue fluid.Atherosclerosis, 2004;176:57-62
- Noto et al, Exclusive association of paraoxonase 1 with high-density lipoprotein particles in apolipoprotein A-I deficiency. Biochem Biophys Res Commun,2001;289:395-401.
Aliases for PON1 Gene
- Paraoxonase 1 2 3 5
- Serum Paraoxonase/Arylesterase 1 3 4
- Serum Aryldialkylphosphatase 1 3 4
- Aromatic Esterase 1 3 4
- Arylesterase 1 2 3
- A-Esterase 1 3 4
- Esterase A 2 3
- PON 1 3 4
- K-45 3 4
- ESA 2 3
- PON 3 4
- Serum Aryldiakylphosphatase 3
- Arylesterase B-Type 3
- Paraoxonase B-Type 3
- EC 3.1.1.81 4
- EC 3.1.1.2 4
- EC 3.1.8.1 4
- MVCD5 3
- PON1 5
