Anti-Rad21(human, hRad21)Antibody, rabbit Antibody

Product#: 70-105
$288.00
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Anti-Rad21 antibody, rabbit polyclonal, affinity purified


General information 

Cat. No. 70-105
Size :50 ug
Antigen Species Human
Host Species Rabbit
Purification Affinity purified with immunogen peptide from rabbit serum of immunized rabbit
Reactivity Reacts with Rad21 of human, mouse and hamster.
Immunogen Human Rad21 C-terminal peptide, C-ATPGPRFHII
Application  
  1. Western blotting (1/2,000 dilution)
  2. Immunofluorescence staining (1/100~1/500 dilution)
Storage Shipped at 4℃. Upon arrival, spin-down and store at -20℃.
Form 1mg/ml in PBS, 50% glycerol. Filter-sterilyzed. Azide- and carrier-free
Data Link UniProtKB/Swiss-Prot O60216 (RAD21_HUMAN)
 

Description 

Rad21 (631 aa, 71 kDa) 2) is a cleavable component of cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate.
 

Anti-Rad21-1.png Anti-Rad21-2.png

Fig.1 Western blot analysis of Rad21 in the whole cell extracts
Anti-Rad21 antibody was used at 1/2,000 dilution. Rad21 migrates as a ~120 kDa protein (Reference)
 
Samples: Crude extracts,10~20 ug
1. HeLa (human)
2. MCF-7 (human)
3. NIH3T3 (mouse)
4. CHO (hamster)
Fig. 2 Immunofluorescence staining of Rad21.
Specific immuno-staining is confirmed by the disappearance of stained Rad21 in the cells transfected with hRad21-specific RNAi
(right-bottom figure).
The cells are extracted in a buffer containing 0.5%Triton X-100 on ice before paraformaldehyde fixation.




 
 

References 

  1. Toyoda Y and Yanagida M. (2006) Coordinated Requirements of Human Topo II and Cohesin for Metaphase Centromere Alignment under Mad2-dependent Spindle Checkpoint Surveillance" Mol. Biol. Cell. 17: 2287-2302 (2006) PMID: 1446084 WB, IF 


Aliases for RAD21 Gene

  • RAD21 Cohesin Complex Component 2 3 5
  • HHR21 2 3 4
  • SCC1 2 3 4
  • Double-Strand-Break Repair Protein Rad21 Homolog 3 4
  • Sister Chromatid Cohesion 1 2 3
  • Nuclear Matrix Protein 1 3 4
  • SCC1 Homolog 3 4
  • KIAA0078 2 4
  • Kleisin 2 3
  • NXP-1 3 4
  • HR21 3 4
  • NXP1 3 4
  • Protein Involved In DNA Double-Strand Break Repair 3
  • RAD21 (S. Pombe) Homolog 2
  • RAD21 Homolog (S. Pombe) 2
  • RAD21 Homolog 3
  • HRAD21 3
  • CDLS4 3
  • RAD21 5
  • MCD1 3
  • MGS 3
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