Yellow Loading Buffer [6X]
Cat No.: DCP-YLB6X
Size: 5 x 1 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description:
Yellow Loading Buffer [6X] is an essential tool in molecular biology laboratories, providing a simple and effective means of loading and tracking DNA samples during gel electrophoresis experiments. It facilitates the loading process by increasing the density of the sample and providing visual tracking. Tris-HCl provides a stable pH environment for the DNA/RNA samples and helps maintain the integrity of nucleic acids during electrophoresis. EDTA acts as a chelating agent and inhibits metal-dependent nucleases, protecting DNA/RNA from degradation. Tartrazine serves as a tracking dye and allows visual monitoring of the electrophoresis progress. Glycerol increases the density of the sample and ensures that the DNA/RNA sample sinks to the bottom of the well when loaded.
* Please note that this buffer contains tartrazine.
Advantages:
- Easy sample loading: The glycerol in the buffer increases the density of DNA samples, ensuring they sink into the gel wells and facilitating easy loading.
- Optimal for specific fragment sizes: It's particularly suitable for DNA fragments between 10 bp and 1 Kb.
- Versatility: It can be used with both agarose and polyacrylamide gels.
- Visual tracking: The yellow dye (Tartrazine) allows for easy monitoring of DNA migration during electrophoresis
Application:
- Loading DNA samples: It is used to load DNA samples onto agarose or polyacrylamide gels for electrophoresis.
- Increasing sample density: The glycerol in the buffer increases the density of DNA samples, ensuring they sink into the gel wells and facilitating easy loading
- Versatility: This buffer is compatible with both agarose and native polyacrylamide gels.
- Compatibility with downstream applications: It doesn't introduce components that might interfere with subsequent analyses of the separated nucleic acids.
Composition:
| Ingredients |
Concentration |
| Glycerol |
30% |
| Tartrazine |
3.6% |
| Tris-HCl |
60 mM |
| EDTA |
60mM |
Quality Control:
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Appearance: Yellow color.
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pH: 7.6
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Sterility: Filtered 0.1 micron Twice and 0.04 micron once in a sterile environment.
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DNase activity: None was detected after incubation of plasmid DNA, and this product was used for 18 hours at room temperature.
-
RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
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Storage: -20 ℃
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Shelf life: 1 year
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of other tracking dyes, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
