Xyltech MSC-01 Xeno-Free(#10401)

Cat. No.: FNK-87-330

Size: 100ml

Storage: -20°C. After thawing, store in a cool, dark place at (2-8°C). Do not refreeze the thawed medium to avoid deterioration of some active ingredients.


Product features:

This product is a culture medium suitable for suppressive growth control of human mesenchymal stem cells (hMSCs). Xyltech™ MSC-01 Xeno-Free can be used to control the growth rate of hMSCs in combination with Xyltech™ Growth MSC, which is a serum-free medium for hMSCs proliferation. This product is *Xeno-Free culture medium. In addition, there is no need to pre-coat culture vessels with any type of attachment matrix.

*Xeno-Free: It contains human-derived components. Any other animal-derived component is free

Precautions for use:

Xyltech™ MSC-01 Xeno-Free does not contain substances that neutralize trypsin activity. When subculturing cells with trypsin, it is strongly recommended that the trypsin activity be sufficiently neutralized with a trypsin inhibitor. Dilution washing alone does not completely remove trypsin activity and the remaining protease activity will reduce subsequent cell growth. This product is a research reagent. It cannot be used for human or animal treatment or diagnostic purposes.

Thawing and preparation:

This product should be thawed at room temperature (20-25°C). Avoid thawing in a water bath. After thawing, store the medium in a cool, dark place (2-8°C) and use within one month. Fine particles may be observed but there is no influence on quality.

Example of cell culture protocol for normal human adipose-derived stem cells (ADSCs) culture using Xyltech™ MSC-02 Animal-Free and Xyltech™ Growth MSC (AF):

Required cells and reagents:

• Normal human ADSCs (100 mm-dish)
• Xyltech™ MSC-01 Xeno-Free (growth suppression medium) *This product
• Xyltech™ Growth MSC Medium (Cat. No. FNK-87-331)
• Xyltech™ Growth MSC Supplement AF (Cat. No. FNK-87-333)
• r-TE (Recombinant Trypsin/EDTA Solution) (Cat. No. FNK-87-974)
• s-TI (Synthetic Trypsin Inhibitor Solution) (Cat. No. FNK-87-975)
• D-PBS (-)

Growth control of normal human ADSCs:

1. Before using Xyltech™ Growth MSC, add the entire amount of Xyltech™ Growth MSC Supplement XF (thawed before use) to Xyltech™ Growth MSC Medium and mix well.
2. Warm the Xyltech™ Growth MSC, D-PBS (-), r-TE, and s-TI in a 37°C water bath.
3. Remove the culture supernatant of selected normal human ADSCs that have reached around 80% confluence (sub confluence).
4. Rinse the cell layer with 5 mL of D-PBS (-).
5. Add 0.5 mL of r-TE and incubate at 37°C for approximately 2 minutes.
6. Add 0.5 mL of s-TI, mix well, gently pipette up and down several times, collect cells from the dish, and centrifuge at 1,000 rpm, for 5 minutes.
7. Aspirate the supernatant and add the appropriate amount of Xyltech™ Growth MSC to resuspend the cells and seed into a new tissue culture dish.
8. The next day, using a phase-contrast microscope to confirm the cells are engrafted and replace the growth medium with the Xyltech™ MSC-01 Xeno-Free culture medium.
9. The cells can be cultured for up to 3 days with Xyltech™ MSC-01 Xeno-Free. The cells start to regrow quickly and become confluent 1-2 days after changing the medium back to the Xyltech™ Growth MSC. Begin subculture and/or experiments with the cells.

Phase contrast microscope images of normal human ADSCs cultured with Xyltech™ Growth MSC (Xeno-Fee) or Xyltech™ MSC-01 Xeno-Free for 3 days

Fig. 1 Xyltech™ Growth MSC 
Fig. 2 Xyltech™ MSC-01 Xeno-Free 

*The protocol is based on experimental results. It may be necessary to adjust seeding density, and passage timing according to the cells. This protocol is intended for research purposes only.


Fig. 1 Adipocyte differentiation (Oil Red O staining)
Fig. 2 Osteoblast differentiation (Alizarin Red S staining)
Fig. 3 Chondrocyte differentiation (Alcian Blue staining)


Human adipose-derived mesenchymal stem cells were cultured for three days using Xyltech™ MSC-01 Xeno-Free (for proliferation control) and then induced to differentiate into various cell types.

It was confirmed that Xyltech™ MSC-01 Xeno-Free can be used to culture cells while suppressing proliferation and maintaining their properties.