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Xylose isomerase A (Glucose isomerase A), Thermostable, 5 ml

Product#: FNK-XYI-75-01-5 ml

$354.22

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Xylose isomerase A (Glucose isomerase A), Thermostable, 5 ml

Cat.No. FNK-XYI-75-01
Lot No. 1-S001
Protein conc. 10 mg/ml
Volume 5 ml
Form 20 mM Tris-HCl (pH 8.0)
Storage -20 ^oC
Activity 150 U/ml
Notes For research use only

EC No. 5.3.1.5
Substrate xylose
Reaction product xylulose
Reaction temperature －
Reaction pH 6.0~8.0

Description

Enzymes work at high temperature with high reactivity The "Thermostable Enzyme".

Generally, enzymes in the living body are denatured and lose activities at the temperature over 50 - 60°C.
However, the thermostable enzymes obtained from special microorganisms (hyper-thermophilic microorganisms) alive at high temperature (over 70°C) have unique characteristics.

Using regular enzymes as a catalyst in chemical reactions are not cost effective due to its low stability and needs of additional enzyme.
By using these thermostable enzymes, we can expect not only cost saving but also other advantages - especially in the field of fine chemicals.

Features of Thermostable Enzyme

Highly stable and durable for long term use
High reactivity at high temperature (70 - 100°C)
Resistance to organic solvent is expected
Special instruments to inhibit bacterial growth are not required.
Reaction speficity is much higher compared with chemical reaction
Speed of reaction can be accelerated

Activity measurement

The enzyme solution was mixed with the pre-warmed substrate solution (5 mM xylose, 50 mM PIPES-KOH, pH 7.0, 1 mM CoCl2). After incubation at 85 oC for 5 min, the produced xylulose was measured by the Cysteine-carbazole method. One unit is defined as the amount of enzyme producing 1 µmol of xylulose per 1 minute.