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FluxMPS™ William's Medium E w/o Sodium Bicarbonate: 1X Liquid

Product#: DCP-WME-B1X
$52.80
DCP-WME-B1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ William's Medium E w/o Sodium Bicarbonate — 1X Liquid

Contains L-Glutamine Contains Phenol Red Contains Calcium Contains Magnesium Contains Glucose (2 g/L) Contains Sodium Pyruvate Without Sodium Bicarbonate

FluxMPS™ DCP-WME-B1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) William's Medium E formulation engineered for primary hepatocytes and related cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. The 0.04 µm final cut-off is five times finer than the 0.22 µm membranes used for conventional sterile filtration. Formulation: [+] 2 g/L Glucose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] Phenol Red | [-] Sodium Bicarbonate.

  • Glucose source: 2000 mg/L (2.0 g/L) — supports hepatocyte energy metabolism
  • Glutathione (reduced), 0.050 mg/L — antioxidant support for redox-sensitive hepatocyte cultures
  • Methyl linoleate (fatty acid), 0.030 mg/L — supports hepatocyte lipid metabolism and CYP450 function
  • Sodium bicarbonate excluded; formulated for CO₂-independent hepatocyte culture with user-added HEPES buffering
  • Quadruple-stage filtration train: 0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm, reaching a 0.04 µm final cut-off
  • Endotoxin release specification: < 0.05 EU/mL (LAL assay, USP <85>), tested per manufacturing batch
  • Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
  • Custom pH, nutrient, and buffer modifications available on request
CAT. NO.
DCP-WME-B1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
William's Medium E — 1X Liquid, w/o Sodium Bicarbonate
  • Media familyWilliam's Medium E
  • Glucose2000 mg/L (2.0 g/L)
  • Formulation[+] L-Glutamine, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose (2 g/L), [+] Sodium Pyruvate | [-] Sodium Bicarbonate
  • AppearanceOrange-red colored, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)240–280 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered William's Medium E passes mycoplasma-scale particulates and subvisible particulates that can disrupt primary hepatocyte function and bile canaliculi formation. FluxMPS™ is built around a validated four-stage filtration train and a batch-tested endotoxin specification to reduce those risks.

filter_alt

Microchannel-safe purity

0.04 µm final filtration reduces particulate load relative to standard 0.22 µm filtration, supporting microchannel and bile-canaliculi geometry in liver-on-chip devices.

science

Hepatocyte-optimized formulation

William's Medium E contains methyl linoleate (fatty acid) and reduced glutathione, supporting primary hepatocyte lipid metabolism and antioxidant defense.

water_drop

Ultrapure-grade water

Type 1 water, 18.2 MΩ·cm, processed for low trace-metal and organic-carbon (TOC) content to minimize feedwater contaminant load.

shield

Batch-tested endotoxin control

Every manufacturing batch is tested and must meet the release specification of < 0.05 EU/mL (LAL assay, USP <85>) before release.

visibility

Low background for imaging

Ultra-low particulate filtration supports confocal and biosensor-based hepatocyte imaging workflows on a particulate baseline; note this formulation contains phenol red (10.700 mg/L), and phenol red–free variants are available on request for fluorescence-sensitive assays.

tune

Customization on demand

pH, nutrient concentrations, HEPES, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages, run as two dedicated prefilter + final-filter pairs, reach a final 0.04 µm polish under ISO Class 5 aseptic fill conditions.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulate, cell debris, and protein aggregates; protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and mycoplasma-scale organisms (0.2–0.3 µm) that pass a standard 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill & finish in a validated ISO Class 5 (Class 100) environment.

Performance vs. conventional media

A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration of William's Medium E.

5.5×
Finer final pore size vs. 0.22 µm membranes (0.22 ÷ 0.04)
0.04
µm final pore size — sub-mycoplasma-scale polishing
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma risk is controlled by 0.1 µm mycoplasma-retentive filtration (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-WME-B1X William's Medium E quadruple-stage filtration system, 0.1 micron x2 plus 0.04 micron x2, for organ-on-a-chip and microfluidic hepatocyte culture, Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-WME-B1X
Applications

Hepatocyte and liver OoC applications

FluxMPS™ DCP-WME-B1X — William's Medium E without sodium bicarbonate — delivers 0.04 µm filtered, CO₂-independent purity for primary hepatocytes and related OoC applications.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available on request for automated bioreactors and robotic perfusion systems.

  • Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates beyond the 0.04 µm cut-off
  • Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion systems
  • Extended Perfusion Stability: Supports consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Hepatology

Primary Hepatocyte Culture

William's Medium E is a standard base for primary hepatocyte culture; methyl linoleate and glutathione support CYP450 activity and albumin synthesis.

Primary hepatocytesHepG2HepaRG
Microfluidics

Liver-on-Chip

0.04 µm filtered William's Medium E delivers fatty acid (methyl linoleate) and antioxidant (glutathione) support for liver-on-chip and hepatocyte OoC bile canaliculi models.

Liver-on-chipBile canaliculiOoC
Toxicology

Hepatotoxicity Assays

Used as a base medium for in vitro hepatotoxicity screening, including CYP450 induction, drug metabolism, and bile acid transport assays.

CYP450Drug metabolismDILI assays
Cancer Biology

Hepatoma Cell Lines

Supports Huh-7, HepG2, and HepaRG hepatoma lines in differentiation protocols and viral hepatitis models.

Huh-7HepG2HepaRG
Metabolomics

Hepatocyte Metabolic Studies

2 g/L glucose plus methyl linoleate supports fatty acid oxidation, lipid metabolism, and hepatic metabolomics assays.

FAO assaysLipid metabolismMetabolomics
Live-Cell Imaging

Hepatocyte Confocal & Imaging

Ultra-low particulate filtration supports hepatocyte confocal microscopy and TEER-monitored liver-on-chip imaging; a phenol red–free variant of this formulation is available on request for fluorescence-sensitive assays.

ConfocalTEERBiosensors
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA available on request: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] L-Glutamine, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose (2 g/L), [+] Sodium Pyruvate | [-] Sodium Bicarbonate
Appearance Orange-red colored, clear solution
Glucose 2000 mg/L (2.0 g/L)
L-Glutamine 292.000 mg/L
Sodium Pyruvate 25.000 mg/L
Phenol Red 10.700 mg/L
pH USP <791> 7.4
Osmolality USP <785> 240–280 mOsm/kg H2O
Total ingredients 50 components across 3 grouped categories
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release spec — see §Manufacturing & Compliance)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protect from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO₂ requirement CO₂-independent; supplement with HEPES (15–25 mM) for pH buffering
Available pack sizes 500 mL, 1000 mL
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

William's Medium E: 50 components verified per lot with CAS numbers for full raw-material traceability. Contains methyl linoleate (fatty acid) and reduced glutathione supporting primary hepatocyte lipid metabolism and antioxidant defense.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Copper sulfate pentahydrate 7758-99-8 0.0001
Ferric nitrate nonahydrate 7782-61-8 0.0001
Manganese chloride tetrahydrate 13446-34-9 97.670
Magnesium sulfate anhydrous 7487-88-9 0.0001
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6000.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 122.000
Zinc sulfate heptahydrate 7446-20-0 0.0002
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 50.000
L-Alanine 56-41-7 90.000
L-Arginine hydrochloride 1119-34-2 60.460
L-Asparagine monohydrate 5794-13-8 20.000
L-Aspartic acid 56-84-8 30.000
L-Cysteine hydrochloride monohydrate 7048-04-6 57.986
L-Cystine dihydrochloride 30925-07-6 26.070
L-Glutamic acid 56-86-0 44.500
L-Glutamine 56-85-9 292.000
L-Histidine hydrochloride monohydrate 5934-29-2 16.409
L-Isoleucine 73-32-5 50.000
L-Leucine 61-90-5 75.000
L-Lysine hydrochloride 657-27-2 87.460
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 25.000
L-Proline 147-85-3 30.000
L-Serine 56-45-1 10.000
L-Threonine 72-19-5 40.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt dihydrate 69847-45-6 50.650
L-Valine 72-18-4 50.000
Component CAS Number mg/L
VITAMINS
Alpha Tocopherol phosphate disodium salt 60934-46-5 0.010
Ascorbic acid sodium salt 134-03-2 2.270
Calciferol 50-14-6 0.100
Choline chloride 67-48-1 1.500
D-Biotin 58-85-5 0.500
D-Ca-Pantothenate hemicalcium 137-08-6 1.000
Folic acid 59-30-3 1.000
Menadione sodium bisulfite 130-37-0 0.010
Niacinamide 98-92-0 1.000
Pyridoxine hydrochloride 58-56-0 1.000
Retinol acetate 127-47-9 0.010
Riboflavin 83-88-5 0.010
Thiamine hydrochloride 67-03-8 1.000
Vitamin B12 68-19-9 0.200
OTHERS
myo-Inositol 87-89-8 2.000
D-Glucose 50-99-7 2000.000
Glutathione reduced 70-18-8 0.050
Methyl linoleate 2777-58-4 0.030
Phenol red sodium salt 34487-61-1 10.700
Sodium pyruvate 113-24-6 25.000
Custom formulation: Contact support@diagnocine.com for DCP-WME-B1X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016-certified quality management system. Final QC at Diagnocine, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm feed water, processed for low trace-metal and organic-carbon content.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — a Certificate of Analysis is issued for every batch.

Endotoxin — USP <85> BET

LAL assay; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL per batch.

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 240–280 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request for any DCP-WME-B1X lot at support@diagnocine.com.
Product Comparison

How DCP-WME-B1X compares

FluxMPS™ DCP-WME-B1X vs. conventional 0.22 µm–filtered William's and standard media.

Parameter DCP-WME-B1X (FluxMPS™) Conventional William's Medium
(0.22 µm filtered)		 Standard DMEM/RPMI
(0.22 µm filtered)
Grade Microfluidics Suitable Not designated Not designated
William's Medium E without Sodium Bicarbonate — CO₂-independent hepatocyte culture for liver-on-chip platforms check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple-stage) 1 1
Mycoplasma barrier filtration check_circle Yes (0.1 µm mycoplasma-retentive) cancel No cancel No
Endotoxin specification < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> Method 1 particulate tested check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatibility check_circle Microfluidics Suitable cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle Available on request cancel Not typically offered cancel Not typically offered

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-WME-B1X — William's Medium E without sodium bicarbonate.

Yes. DCP-WME-B1X is processed through a quadruple-stage filtration system reaching a 0.04 µm final pore size, delivering ultra-low particulate levels for MPS, OoC, and LoC platforms. William's Medium E with 0.04 µm filtration is well suited to specialized OoC hepatocyte models requiring this formulation.
FluxMPS™ uses four sequential filtration stages — 0.1 µm prefiltration I, 0.04 µm final filtration I, 0.1 µm prefiltration II, and 0.04 µm final filtration II (polish) — reaching a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used in conventional filtration. Mycoplasma risk is controlled by 0.1 µm mycoplasma-retentive filtration; this is not a per-lot test result.
Sodium bicarbonate is excluded to support CO₂-independent hepatocyte culture; add HEPES (15–25 mM) for pH buffering as needed. This enables primary hepatocyte OoC systems in atmospheric environments — particularly relevant for portable liver-on-chip devices, point-of-care hepatotoxicity assays, and hepatocyte perfusion platforms where stable CO₂ is not available. Contact support@diagnocine.com for a bicarbonate-buffered variant if your application requires 5% CO₂.
No. This formulation is CO₂-independent; supplement with HEPES (15–25 mM) for pH buffering in ambient atmosphere.
Yes. FBS (5–10%), serum-free supplements, growth factors, or antibiotics can be added as required. For serum or other protein-containing additions, pre-filter through a 0.2 µm low-protein-binding PES or PVDF membrane before combining with the medium; do not use a 0.04 µm filter for these additions, as it retains serum proteins and lipoproteins. Contact support@diagnocine.com for custom co-formulation.
Every manufacturing batch is tested by LAL assay (USP <85>, assay sensitivity 0.005 EU/mL) and must meet the release specification of < 0.05 EU/mL before release. This is a batch-level specification, not a per-unit certificate.
Yes. Full CoA per batch covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma filtration status, particulate count (USP <788> Method 1), lot number, expiry, and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting William's Medium E in hepatocyte culture and liver OoC applications.

  1. Williams GM, et al. A new cell culture system for adult rat hepatocytes. Exp Cell Res. 1977;106:89–93. doi:10.1083/jcb.1.3.273
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  6. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  7. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  8. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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