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FluxMPS™ William's Medium E: 1X Liquid

Product#: DCP-WME1X
$52.80
DCP-WME1X
Availability:
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ William's Medium E: 1X Liquid

Contains L-Glutamine Contains Sodium Bicarbonate Contains Phenol Red Contains Calcium Contains Magnesium Contains Glucose (2 g/L) Contains Sodium Pyruvate

FluxMPS™ DCP-WME1X is a Microfluidics Suitable, quadruple-stage ultra-filtered William's Medium E formulation engineered for primary hepatocyte, liver-on-chip, and hepatoma cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. Processed through a Quadruple-stage filtration train (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final cut-off — five times finer than the 0.22 µm membranes used in conventional sterile filtration. Formulation: [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose (2 g/L), [+] Sodium Pyruvate.

  • Glucose held at 2000 mg/L (2.0 g/L) — a physiologically appropriate, low-glucose level for primary hepatocyte metabolism
  • Glutathione (reduced), 0.050 mg/L — antioxidant support for redox-sensitive hepatocyte culture
  • Methyl linoleate (fatty acid), 0.030 mg/L — supports hepatocyte lipid metabolism and CYP450 function
  • Quadruple-stage filtration: 0.1 µm prefiltration I → 0.04 µm final filtration I → 0.1 µm prefiltration II → 0.04 µm final filtration II (polish)
  • Endotoxin release specification: < 0.05 EU/mL (USP <85>, LAL), tested per manufacturing batch
  • Ultrapure Type 1 water (18.2 MΩ·cm) with controlled trace metals and low TOC; manufactured under an ISO 13485:2016 quality management system
  • 51 ingredients across Inorganic Salts, Amino Acids, Vitamins and Others — full composition and CAS numbers below
  • pH, salts, HEPES and nutrient composition available on request — contact support@diagnocine.com
CAT. NO.
DCP-WME1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
William's Medium E — 1X Liquid
  • Media familyWilliam's Medium E
  • Formulation[+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose (2 g/L), [+] Sodium Pyruvate
  • Glucose2000 mg/L (2.0 g/L)
  • AppearanceOrange-red colored, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)Contact for specification
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered William's Medium E can pass mycoplasma, subvisible particulates, and endotoxin fragments that disrupt primary hepatocyte function and bile canaliculi formation. FluxMPS™ is engineered to reduce these risks.

filter_alt

Microchannel-safe purity

0.04 µm final filtration and USP <788> particulate compliance support particle-free delivery, preserving bile canaliculi geometry in liver-on-chip models.

science

Hepatocyte-optimized formulation

William's Medium E contains methyl linoleate (fatty acid) and reduced glutathione, supporting primary hepatocyte lipid metabolism and antioxidant defense.

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Ultrapure-grade water

Type 1 water (18.2 MΩ·cm) with controlled trace metals and low organic carbon (TOC) supports consistent, reproducible hepatocyte culture performance batch to batch.

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Low-endotoxin release specification

< 0.05 EU/mL (USP <85>, LAL assay), tested per manufacturing batch — helps reduce the risk of LPS-driven Kupffer cell activation in hepatocyte co-culture models.

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Rich, hepatocyte-specific nutrient profile

A specialized amino acid, vitamin, fatty acid, and antioxidant profile for demanding hepatocyte-derived cell types — delivered particle-free for OoC use.

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Customization on demand

pH, nutrient concentrations, HEPES, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages — two dedicated prefilter/final-filter pairs — reaching a final 0.04 µm polish.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulates, cell debris, and protein aggregates; protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; 0.1 µm-and-larger mycoplasma-retentive filtration and retention of sub-micron particulates/microaggregates that pass a 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    A second, dedicated prefilter protecting the second 0.04 µm cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill & finish.

Performance vs. conventional media

FluxMPS™ DCP-WME1X is processed through a quadruple-stage filtration train (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final cut-off — five times finer than the 0.22 µm membranes used in conventional sterile filtration — with 0.1 µm mycoplasma-retentive filtration applied at every production stage.

5×
Finer pore size than 0.22 µm conventional filtration
0.04
µm Final pore size — sub-mycoplasma polishing
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma risk is controlled via 0.1 µm mycoplasma-retentive filtration at every production stage (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ DCP-WME1X William's Medium E Quadruple-stage filtration system (0.1 micron x2 + 0.04 micron x2) for organ-on-a-chip, liver-on-chip and microfluidic primary hepatocyte culture applications | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) — DCP-WME1X William's Medium E, engineered for primary hepatocyte and liver-on-chip microfluidic applications.
© Diagnocine® — DCP-WME1X
Applications

Hepatocyte and liver OoC applications

FluxMPS™ DCP-WME1X — William's Medium E — delivers 0.04 µm filtered purity for primary hepatocyte and related OoC applications.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) MPS Grade, ultra nano-filtered variant of this formulation is available on request for automated bioreactors and robotic liquid-handling systems.

  • Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates the 0.04 µm Microfluidics Suitable tier does not target
  • Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion systems
  • Extended Perfusion Stability: Supports consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Hepatology

Primary Hepatocyte Culture

William's Medium E is a standard base for primary hepatocyte culture — methyl linoleate and glutathione support CYP450 activity and albumin synthesis.

Primary hepatocytesHepG2HepaRG
Microfluidics

Liver-on-Chip

0.04 µm filtered William's Medium E delivers fatty acid (methyl linoleate) and antioxidant (glutathione) support for liver-on-chip and hepatocyte OoC bile canaliculi models.

Liver-on-chipBile canaliculiOoC
Toxicology

Hepatotoxicity Assays

A standard base for in vitro hepatotoxicity screening — CYP450 induction, drug metabolism, and bile acid transport assays.

CYP450Drug metabolismDILI assays
Cancer Biology

Hepatoma Cell Lines

Supports Huh-7, HepG2, and HepaRG hepatoma lines in differentiation protocols and viral hepatitis models.

Huh-7HepG2HepaRG
Metabolomics

Hepatocyte Metabolic Studies

Low glucose (2 g/L) plus methyl linoleate supports fatty acid oxidation, lipid metabolism, and hepatic metabolomics assays.

FAO assaysLipid metabolismMetabolomics
Live-Cell Imaging

Low background for imaging

Ultra-low particulate filtration (0.04 µm) reduces particulate interference in hepatocyte confocal microscopy and TEER-monitored liver-on-chip assays. This formulation contains phenol red; a phenol red–free variant is available on request for optical background–sensitive imaging applications.

ConfocalTEERBiosensors
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose (2 g/L), [+] Sodium Pyruvate
Appearance Orange-red colored, clear solution
Glucose 2000 mg/L (2.0 g/L)
L-Glutamine 292.000 mg/L
Sodium Pyruvate 25.000 mg/L
Phenol Red 10.700 mg/L
pH USP <791> 7.4
Osmolality USP <785> Contact for specification
Total ingredients 51
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protect from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement This formulation's sodium bicarbonate content (80 mg/L; ≈1 mM) is substantially below the level used in standard 5% CO2-buffered media, implying near-atmospheric CO2 buffering capacity. Validate CO2 tension empirically for your protocol, or contact support@diagnocine.com for a bicarbonate-adjusted formulation.
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

William's Medium E: 51 ingredients verified per lot with CAS numbers for full raw-material traceability. William's Medium E contains methyl linoleate (fatty acid) and glutathione supporting primary hepatocyte lipid metabolism and antioxidant defense.

INORGANIC SALTS
Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Copper sulfate pentahydrate 7758-99-8 0.0001
Ferric nitrate nonahydrate 7782-61-8 0.0001
Manganese chloride tetrahydrate 13446-34-9 97.670
Magnesium sulfate anhydrous 7487-88-9 0.0001
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 80.000
Sodium chloride 7647-14-5 6000.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 122.000
Zinc sulfate heptahydrate 7446-20-0 0.0002
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 50.000
L-Alanine 56-41-7 90.000
L-Arginine hydrochloride 1119-34-2 60.460
L-Asparagine monohydrate 5794-13-8 20.000
L-Aspartic acid 56-84-8 30.000
L-Cysteine hydrochloride monohydrate 7048-04-6 57.986
L-Cystine dihydrochloride 30925-07-6 26.070
L-Glutamic acid 56-86-0 44.500
L-Glutamine 56-85-9 292.000
L-Histidine hydrochloride monohydrate 5934-29-2 16.409
L-Isoleucine 73-32-5 50.000
L-Leucine 61-90-5 75.000
L-Lysine hydrochloride 657-27-2 87.460
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 25.000
L-Proline 147-85-3 30.000
L-Serine 56-45-1 10.000
L-Threonine 72-19-5 40.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt dihydrate 69847-45-6 50.650
L-Valine 72-18-4 50.000
Component CAS Number mg/L
VITAMINS
Alpha Tocopherol phosphate disodium salt 60934-46-5 0.010
Ascorbic acid sodium salt 134-03-2 2.270
Calciferol 50-14-6 0.100
Choline chloride 67-48-1 1.500
D-Biotin 58-85-5 0.500
D-Ca-Pantothenate hemicalcium 137-08-6 1.000
Folic acid 59-30-3 1.000
Menadione sodium bisulfite 130-37-0 0.010
Niacinamide 98-92-0 1.000
Pyridoxine hydrochloride 58-56-0 1.000
Retinol acetate 127-47-9 0.010
Riboflavin 83-88-5 0.010
Thiamine hydrochloride 67-03-8 1.000
Vitamin B12 68-19-9 0.200
myo-Inositol 87-89-8 2.000
OTHERS
D-Glucose 50-99-7 2000.000
Glutathione reduced 70-18-8 0.050
Methyl linoleate 2777-58-4 0.030
Phenol red sodium salt 34487-61-1 10.700
Sodium pyruvate 113-24-6 25.000
Custom formulation: Contact support@diagnocine.com for DCP-WME1X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

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ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016-certified quality management system. Final QC at the Diagnocine R&D Center, Totowa, NJ, USA.

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Ultrapure Type 1 Water

18.2 MΩ·cm, with controlled trace metals and low organic carbon (TOC).

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending; Certificate of Analysis available for every lot.

Endotoxin — USP <85> BET

LAL assay; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL, tested per batch.

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: Contact for specification.

Documentation & CoA

Full CoA with raw-material traceability available on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-WME1X compares

FluxMPS™ DCP-WME1X vs. conventional 0.22 µm–filtered William's formulations.

Parameter DCP-WME1X (FluxMPS™) Conventional William's Medium
(0.22 µm filtered)		 Standard DMEM/RPMI
(0.22 µm filtered)
Grade Microfluidics Suitable Standard grade Standard grade
William's Medium E — methyl linoleate + glutathione for primary hepatocyte, liver-on-chip, and CYP450 OoC assays check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma barrier filtration check_circle Yes (0.1 µm mycoplasma-retentive) cancel No cancel No
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate tested check_circle Yes (Method 1) cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatibility check_circle Microfluidics Suitable cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle Available on request cancel Fixed catalog SKU cancel Fixed catalog SKU

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-WME1X — William's Medium E.

Yes. DCP-WME1X is processed through a Quadruple-stage filtration system reaching a 0.04 µm final pore size, delivering ultra-low particulate levels appropriate for MPS, OoC, and LoC platforms. William's Medium E with 0.04 µm filtration is a Microfluidics Suitable formulation suited to liver-on-chip and hepatocyte OoC models.
FluxMPS™ uses four sequential filters — 0.1 µm prefiltration I, 0.04 µm final filtration I, 0.1 µm prefiltration II, and 0.04 µm final filtration II (polish) — reaching a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used in conventional sterile filtration, with 0.1 µm mycoplasma-retentive filtration applied at every production stage.
William's Medium E was developed specifically for primary hepatocyte culture, and 2 g/L glucose is physiologically appropriate for hepatocyte metabolism (compared to 4.5 g/L in standard DMEM). Methyl linoleate and glutathione are included to support hepatocyte-specific lipid metabolism and antioxidant defense. If your protocol requires a different glucose level, contact support@diagnocine.com for a custom formulation.
This formulation's sodium bicarbonate content (80 mg/L; approximately 1 mM) is substantially below the level used in standard 5% CO₂-buffered media, implying near-atmospheric CO₂ buffering capacity. We recommend validating CO₂ tension empirically for your hepatocyte culture protocol; contact support@diagnocine.com for a bicarbonate-adjusted formulation if 5% CO₂ compatibility is required.
Yes. Serum, growth factors, and other protein-containing supplements should be added using a 0.2 µm low-protein-binding PES or PVDF syringe filter — never 0.04 µm, which retains lipoproteins and reduces serum efficacy. Contact support@diagnocine.com for custom co-formulation.
FluxMPS™ DCP-WME1X is released against a specification of < 0.05 EU/mL by LAL assay (USP <85>). Endotoxin is controlled per manufacturing batch: every batch is tested before release and must meet this specification. A Certificate of Analysis stating the batch result is available on request.
Yes. A full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma filtration status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting William's Medium E in hepatocyte culture and liver OoC applications.

  1. Williams GM, Gunn JM. Long-term cell culture of adult rat liver epithelial cells. Exp Cell Res. 1974;89:139–142. doi:10.1016/0014-4827(74)90201-8
  2. Guguen-Guillouzo C, Guillouzo A. General review on in vitro hepatocyte models. Methods Mol Biol. 2010;640:1–40. doi:10.1007/978-1-60761-688-7_1
  3. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  4. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  5. Kietzmann T. Metabolic zonation of the liver: the oxygen gradient revisited. Redox Biol. 2017;11:622–630. doi:10.1016/j.redox.2017.01.012
  6. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  7. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  8. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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