FluxMPS™ Waymouth Medium: 1X Liquid
FluxMPS™ Waymouth Medium: 1X Liquid
FluxMPS™ DCP-WMBM1X is a Microfluidics Suitable, quadruple-stage ultra-filtered Waymouth Medium MB 752/1 formulation engineered for mammalian cell culture — including fibroblast, hybridoma and myeloma models — on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. Formulation: [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose (5 g/L).
- Waymouth Medium MB 752/1 formulation with 5000 mg/L (5.0 g/L) D-glucose as the carbon source
- Glutathione (reduced), 15 mg/L — supports antioxidant/redox balance in fibroblast and hybridoma clonal growth
- L-Glutamine (350 mg/L) and sodium bicarbonate (2240 mg/L) buffering system
- Quadruple-stage filtration train: 0.1 µm ×2 + 0.04 µm ×2, reaching a 0.04 µm final cut-off
- Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85>)
- Manufactured under an ISO 13485:2016 quality management system; final QA at Diagnocine, Totowa, NJ
- Contains phenol red sodium salt (11 mg/L) as a visible pH indicator — red-colored, clear solution
- Available in 500 mL and 1000 mL sizes; custom pH, nutrient and buffer modifications available on request
- Media familyWaymouth Medium MB 752/1
- Glucose5000 mg/L (5.0 g/L)
- Formulation[+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose (5 g/L)
- AppearanceRed-colored, clear solution
- pH (USP <791>)7.4
- Osmolality (USP <785>)Contact for specification
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
- Storage2–8°C, protect from light
- Shelf Life12 months from date of manufacture, unopened
Engineered where standard media fails
Conventional 0.22 µm–filtered Waymouth Medium passes mycoplasma, subvisible particulates, and clumped debris that clog microfluidic channels and confound optical/electrical readouts. FluxMPS™ is built for these failure modes.
Microchannel-safe purity
0.04 µm final filtration with USP <788> Method 1 (light obscuration) particulate compliance — formulated for safe perfusion across chip geometries.
Total metabolic control
5 g/L D-glucose carbon source plus reduced glutathione give you a defined, adjustable metabolic starting point for clonal and hybridoma growth studies.
Ultrapure-grade water
Ultrapure Type 1 water (18.2 MΩ·cm) supports low trace-metal and organic-carbon (TOC) background for sensitive cell culture formulations.
Low background for imaging
Quadruple-stage filtration lowers the particulate baseline for confocal and biosensor work. Note: this formulation contains phenol red sodium salt (11 mg/L) and is therefore not suited to phenol-red–sensitive fluorescence assays — a phenol-red–free custom formulation is available on request.
Rich, stable nutrient profile
Waymouth Medium MB 752/1 carries a full complement of amino acids, vitamins, and glutathione, released per micro-batch lot.
Customization on demand
pH, nutrient concentrations, HEPES, and component modifications available. Contact support@diagnocine.com.
Quadruple-stage filtration system
Four serial filtration stages — two dedicated prefilter + final-filter pairs — reaching a final 0.04 µm polish.
-
1
0.1 µm Prefiltration I — Large Particulate & Debris Removal
Removes large aggregates and cell debris; protects the first 0.04 µm final-filter cartridge.
-
2
0.04 µm Final Filtration I — Mycoplasma-Retentive Barrier
Retains particulates and organisms in the mycoplasma size range (0.2–0.3 µm), well below the 0.22 µm rating of conventional sterile filters.
-
3
0.1 µm Prefiltration II — Second Cartridge Protection
A second, dedicated 0.1 µm prefilter protects the second 0.04 µm cartridge downstream.
-
4
0.04 µm Final Filtration II — Polish & Aseptic Fill
Final 0.04 µm polishing pass followed by aseptic fill & finish under ISO Class 5 (Class 100) conditions.
Performance vs. conventional media
FluxMPS™ DCP-WMBM1X is filtered through two dedicated 0.1 µm + 0.04 µm pairs in series, reaching a 0.04 µm final cut-off — five times finer than the 0.22 µm membranes used in conventional Waymouth Medium filtration.
© Diagnocine® — DCP-WMBM1X
Specialized cell culture applications
FluxMPS™ DCP-WMBM1X — Waymouth Medium MB 752/1 — delivers 0.04 µm filtered, Microfluidics Suitable purity for mammalian cell culture and related OoC applications.
Automated Bioreactors & Robotics
An optional 0.01 µm (10 nm) MPS Grade ultra nano-filtered variant of this formulation is available on request for automated bioreactor and robotic perfusion systems.
- Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates beyond the Microfluidics Suitable tier
- Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion systems
- Extended Perfusion Stability: Supports consistent nutrient delivery over long-duration culture
Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.
CHO & Mammalian Cell Culture
Optimized for CHO, hybridoma, myeloma, and primary fibroblast culture in serum-supplemented or low-serum conditions.
Organ-on-a-Chip & MPS
0.04 µm filtered, Microfluidics Suitable media reduces microchannel clogging risk in multi-organ chip architectures.
Cancer Cell Lines
Supports fibroblast co-culture tumor models and primary cancer cell culture in a defined, glucose-rich nutrient environment.
Primary & Stem Cell Support
Rich nutrient and trace-element profile supports primary cell viability and stem cell maintenance in low-serum conditions.
Metabolic Flux Analysis
Defined glucose/glutamine nutrient base supports ¹³C isotope tracing studies. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red–free medium.
Microscopy & Optical Sensing
Reduced particulate baseline supports confocal and biosensor work; note this formulation contains phenol red (not suited to phenol-red–sensitive fluorescence).
Analytical release specifications
Every lot released against the full specification matrix. Available sizes: 500 mL, 1000 mL. CoA: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose (5 g/L) |
| Appearance | Red-colored, clear solution |
| Glucose | 5000 mg/L (5.0 g/L) |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | Contact for specification |
| Total ingredients | 41 components |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | 0.04 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> Method 1 | NMT 25/mL |
| Particulate ≥25 µm USP <788> Method 1 | NMT 3/mL |
| Water purity | Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, away from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO₂ requirement | Approximately 5.9% CO₂ (derived from 2240 mg/L NaHCO₃ buffering at pH 7.4; validate per instrument) |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
Waymouth Medium MB 752/1: 41 components verified per lot with CAS numbers for raw-material traceability. Contains reduced glutathione, supporting fibroblast and hybridoma antioxidant defense.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 120.000 |
| Disodium hydrogen phosphate anhydrous | 7558-79-4 | 300.000 |
| Magnesium chloride anhydrous | 7786-30-3 | 112.560 |
| Magnesium sulfate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 150.000 |
| Potassium dihydrogen phosphate | 7778-77-0 | 80.000 |
| Sodium bicarbonate | 144-55-8 | 2240.000 |
| Sodium chloride | 7647-14-5 | 6000.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 50.000 |
| L-Arginine hydrochloride | 1119-34-2 | 75.000 |
| L-Aspartic acid | 56-84-8 | 60.000 |
| L-Cysteine hydrochloride monohydrate | 7048-04-6 | 90.000 |
| L-Cystine dihydrochloride | 30925-07-6 | 19.550 |
| L-Glutamic acid | 56-86-0 | 150.000 |
| L-Glutamine | 56-85-9 | 350.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 150.000 |
| L-Isoleucine | 73-32-5 | 25.000 |
| L-Leucine | 61-90-5 | 50.000 |
| L-Lysine hydrochloride | 657-27-2 | 240.000 |
| L-Methionine | 63-68-3 | 50.000 |
| L-Phenylalanine | 63-91-2 | 50.000 |
| L-Proline | 147-85-3 | 50.000 |
| L-Threonine | 72-19-5 | 75.000 |
| L-Tryptophan | 73-22-3 | 40.000 |
| L-Tyrosine disodium salt dihydrate | 69847-45-6 | 57.660 |
| L-Valine | 72-18-4 | 65.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Ascorbic acid | 50-81-7 | 17.500 |
| Biotin | 58-85-5 | 0.020 |
| Choline chloride | 67-48-1 | 250.000 |
| D-Ca-Pantothenate | 137-08-6 | 1.000 |
| Folic acid | 59-30-3 | 0.400 |
| Niacinamide | 98-92-0 | 1.000 |
| Pyridoxine hydrochloride | 58-56-0 | 1.000 |
| Riboflavin | 83-88-5 | 1.000 |
| Thiamine hydrochloride | 67-03-8 | 10.000 |
| Vitamin B12 | 68-19-9 | 0.200 |
| Hypoxanthine sodium salt | 45738-97-4 | 29.000 |
| OTHERS | ||
| i-Inositol | 87-89-8 | 1.000 |
| D-Glucose | 50-99-7 | 5000.000 |
| Glutathione reduced | 70-18-8 | 15.000 |
| Phenol red sodium salt | 34487-61-1 | 11.000 |
Manufacturing & compliance
Every FluxMPS™ product is manufactured and released under a rigorous multi-layer quality system.
ISO 13485:2016 Quality Management
Manufactured under an ISO 13485:2016–certified quality management system. Final QA at the Diagnocine R&D Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm feed water supports low trace-metal and organic-carbon (TOC) background.
ISO Class 5 Fill & Finish
Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.
Micro-Batch Precision
Small-batch, per-lot tested — no blending; a Certificate of Analysis is available for every lot.
Endotoxin — USP <85> BET
LAL assay; release specification < 0.05 EU/mL per batch.
Particulate — USP <788> Method 1
Light obscuration; NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).
Osmolality — USP <785>
Target: Contact for specification.
Documentation & CoA
Full CoA with raw-material traceability available on request.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-WMBM1X compares
FluxMPS™ DCP-WMBM1X vs. conventional 0.22 µm–filtered Waymouth and DMEM/RPMI formulations.
| Parameter | DCP-WMBM1X (FluxMPS™) | Conventional Waymouth Medium (0.22 µm filtered) |
Standard DMEM/RPMI (0.22 µm filtered) |
|---|---|---|---|
| Grade | Microfluidics Suitable | Standard grade | Standard grade |
| Waymouth Medium MB 752/1 full formulation with 5 g/L glucose and glutathione for hybridoma/fibroblast OoC use | check_circle Yes | cancel No | cancel No |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple-stage) | 1 | 1 |
| Mycoplasma-retentive filtration | check_circle Yes (0.04 µm) | cancel No | cancel No |
| Endotoxin (release specification) | < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP <788> particulate compliance | check_circle Yes (Method 1) | cancel No | cancel No |
| Water quality | Type 1, 18.2 MΩ·cm | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatibility | check_circle Microfluidics Suitable | cancel Risk of clogging | cancel Risk of clogging |
| Custom formulation | check_circle Yes | cancel No | cancel No |
Comparison figures from published supplier specifications, accessed 2 September 2026. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ DCP-WMBM1X — Waymouth Medium MB 752/1.
Supporting literature
Key publications supporting Waymouth Medium MB 752/1 in specialized cell culture and OoC applications.
- Waymouth C. Rapid proliferation of sublines of NCTC clone 929 (strain L) mouse cells in a simple chemically defined medium (MB 752/1). J Natl Cancer Inst. 1959;22:1003–1017. doi:10.1083/jcb.1.3.273
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
- Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
