FluxMPS™ Waymouth Medium

verified ISO 13485 Certified Manufacturing

FluxMPS™ Waymouth Medium

FluxMPS™ DCP-WMBM-B1X is an MPS-grade, ultra-filtered Waymouth Medium MB 752/1 formulation engineered for Mammalian cell culture and related cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. Processed through a Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2), it delivers approximately 5× lower particulate counts than conventional 0.22 µm–filtered media. Formulation: [+] 5 g/L Glucose, [+] L-Glutamine | [-] Sodium Bicarbonate.

Glucose source: 5000 mg/L (5.0 g/L)
Glutathione (reduced) included — antioxidant support for redox-sensitive cell types
0.04 µm final nano-filtration — sub-mycoplasma purity; < 0.05 EU/mL endotoxin
Quadruple-stage filtration: 0.1 µm pre-I → 0.04 µm pre-II → 0.1 µm sterile-I → 0.04 µm final polish
Ultrapure Type 1 water (18.2 MΩ·cm), ISO 13485:2016 QMS, ISO Class 5 fill

CAT. NO.

DCP-WMBM-B1X | UNSPSC 41122100 Cell Culture Media

Waymouth Medium — 1X Liquid

Media familyWaymouth Medium MB 752/1
Glucose5000 mg/L (5.0 g/L)
Formulation[+] 5 g/L Glucose, [+] L-Glutamine | [-] Sodium Bicarbonate
AppearanceRed colored, clear solution
pH (USP <791>)7.4
Osmolality (USP <785>)240.00–345.00 mOsm/kg H₂O
Endotoxin (USP <85>)< 0.05 EU/mL
Filtration0.1 µm ×2 + 0.04 µm ×2
Shelf Life12 months / 2–8°C

ISO 13485:2016 USP <85> <785> <788> RUO

Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered Waymouth passes mycoplasma, subvisible particulates, and endotoxin fragments that clog microfluidic channels and activate innate immune signaling. FluxMPS™ eliminates these failure modes.

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Microchannel-safe purity

0.04 µm final filtration; USP <788> particulate compliance. Safe perfusion in every chip geometry.

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Cell-type–optimized formulation

Waymouth Medium contains glutathione (reducing agent) supporting fibroblast and hybridoma antioxidant defense.

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Ultrapure-grade water

18.2 MΩ·cm — eliminates ionic contaminants that interfere with specialized cell culture requirements.

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Below TLR4 endotoxin threshold

< 0.05 EU/mL — prevents LPS-driven inflammatory activation in primary cells.

analytics

Rich nutrient profile

Waymouth contains specialized nutrients for demanding cell types — delivered particle-free for OoC use.

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Customization on demand

pH, nutrient concentrations, HEPES, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages reaching a final 0.04 µm polish under ISO Class 5 aseptic conditions.

1

0.1 µm Pre-filtration I — Large Particulate Removal

Removes large aggregates; protects downstream 0.04 µm membranes.
2

0.04 µm Pre-filtration II — Mycoplasma Barrier

Retains mycoplasma (0.1–0.3 µm) — absent from standard 0.22 µm filtration.
3

0.1 µm Sterile-filtration I — Second-pass Redundancy

Second-pass sterility redundancy; no breakthrough from Stage 1.
4

0.04 µm Sterile-filtration II — Final Polish

Ultimate sub-mycoplasma polish; ISO Class 5 aseptic fill & finish.

Performance vs. conventional media

FluxMPS™ DCP-WMBM-B1X delivers approximately 5× lower particulate counts than standard 0.22 µm filtered Waymouth, with confirmed mycoplasma barrier at every production stage.

5×

Cleaner than 0.22 µm media by particulate count

0.04

µm Final pore size — sub-mycoplasma polishing

Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>); mycoplasma-negative by 0.04 µm barrier every lot.

FluxMPS-Quadruple-stage-filtration-system-Cell-Culture-Media-Diagnocine-V2.jpg — **Figure 1.** FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-WMBM-B1X

Applications

Specialized cell culture applications

FluxMPS™ DCP-WMBM-B1X — Waymouth Medium MB 752/1 — delivers 0.04 µm filtered purity for Mammalian cell culture and related OoC applications.

Automated Bioreactors & Robotics

Next-Generation System Uptime

Optional 0.01 µm (10 nm) ultra-filtered variant available on request.

Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
Valve & Sensor Protection: Eliminates micro-fouling in automated perfusion systems
Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm variant.

Bioproduction

CHO & Mammalian Cell Culture

Optimized for CHO, hybridoma, myeloma, and primary fibroblast culture in serum-free or low-serum conditions.

CHOHybridomaMyeloma

Microfluidics

Organ-on-a-Chip & MPS

0.04 µm filtered media prevents microchannel clogging in complex multi-organ chip architectures.

OoCToCMPS

Cancer Biology

Cancer Cell Lines

Supports fibroblast co-culture tumor models and primary cancer cell culture in the defined nutrient environment of specialized media.

Tumor modelsFibroblastsCo-culture

Stem Cell Biology

Primary & Stem Cell Support

Rich nutrient profile with trace elements supports primary cell viability and stem cell maintenance in low-serum conditions.

Primary cellsMSCsiPSC co-culture

Metabolomics

Metabolic Flux Analysis

Defined nutrient base for ¹³C isotope tracing and Seahorse XF assays in specialized cell culture models.

¹³C tracingSeahorse XFMetabolomics

Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate; phenol red–free variants for autofluorescence-free confocal and biosensor applications.

ConfocalTEERBiosensors

Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters

Parameter	Specification
Formulation	[+] 5 g/L Glucose, [+] L-Glutamine \| [-] Sodium Bicarbonate
Appearance	Red colored, clear solution
Glucose	5000 mg/L (5.0 g/L)
pH USP <791>	7.4
Osmolality USP <785>	240.00–345.00 mOsm/kg H₂O
Total ingredients	40

Sterility, Purity & Safety

Parameter	Specification
Endotoxin USP <85> BET	< 0.05 EU/mL
Sterility USP <71>	No growth / 14 days
Mycoplasma	Negative (0.04 µm barrier)
Particulate ≥10 µm USP <788>	NMT 25/mL
Particulate ≥25 µm USP <788>	NMT 3/mL
Water purity	Type 1, 18.2 MΩ·cm
Manufacturing std.	ISO 13485:2016
Fill environment	ISO Class 5 (Class 100)

Storage, Handling & Logistics

Parameter	Specification
Storage temperature	2–8°C, away from light
Freeze-thaw	Do not freeze
Shelf life	12 months from manufacture
Shipping condition	cold pack
CO₂ requirement	CO₂-independent; supplement with HEPES (15–25 mM) for pH buffering

Raw Materials & Regulatory

Parameter	Specification
Raw material grade	Reagent / cell culture grade
Traceability	Full lot traceability per ISO 13485
Manufacturing QMS ISO	ISO 13485:2016 certified
Regulatory alignment	21 CFR Part 820 (cGMP) aligned
Production method	Micro-batch, per-lot QC release
Intended use	Research Use Only (RUO)

Formulation

Full composition (mg/L)

Waymouth Medium MB 752/1: 40 ingredients verified per lot with CAS numbers for full raw-material traceability. Waymouth Medium contains glutathione (reducing agent) supporting fibroblast and hybridoma antioxidant defense.

Component	CAS Number	mg/L
INORGANIC SALTS
Calcium chloride dihydrate	10035-04-8	120.000
Disodium hydrogen phosphate anhydrous	7558-79-4	300.000
Magnesium chloride anhydrous	7786-30-3	112.560
Magnesium sulphate anhydrous	7487-88-9	97.720
Potassium chloride	7447-40-7	150.000
Potassium dihydrogen phosphate	7778-77-0	80.000
Sodium chloride	7647-14-5	6000.000

Component	CAS Number	mg/L
AMINO ACIDS
Glycine	56-40-6	50.000
L-Arginine hydrochloride	1119-34-2	75.000
L-Aspartic acid	56-84-8	60.000
L-Cysteine hydrochloride monohydrate	7048-04-6	90.000
L-Cystine dihydrochloride	30925-07-6	19.550
L-Glutamic acid	56-86-0	150.000
L-Glutamine	56-85-9	350.000
L-Histidine hydrochloride monohydrate	5934-29-2	150.000
L-Isoleucine	73-32-5	25.000
L-Leucine	61-90-5	50.000
L-Lysine hydrochloride	657-27-2	240.000
L-Methionine	63-68-3	50.000
L-Phenylalanine	63-91-2	50.000
L-Proline	147-85-3	50.000
L-Threonine	72-19-5	75.000
L-Tryptophan	73-22-3	40.000
L-Tyrosine disodium salt dihydrate	69847-45-6	57.660
L-Valine	72-18-4	65.000

Component	CAS Number	mg/L
VITAMINS
Ascorbic acid	50-81-7	17.500
Biotin	58-85-5	0.020
Choline chloride	67-48-1	250.000
D-Ca-Pantothenate	137-08-6	1.000
Folic acid	59-30-3	0.400
Niacinamide	98-92-0	1.000
Pyridoxine hydrochloride	58-56-0	1.000
Riboflavin	83-88-5	1.000
Thiamine hydrochloride	67-03-8	10.000
Vitamin B12	68-19-9	0.200
i-Inositol	87-89-8	1.000
Hypoxanthine sodium salt		29.000
OTHERS
D-Glucose	50-99-7	5000.000
Glutathione reduced	70-18-8	15.000
Phenol red sodium salt	34487-61-1	11.000

Custom formulation: Contact support@diagnocine.com for DCP-WMBM-B1X modifications.

Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product manufactured and released under a rigorous multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016–certified and CE-approved facilities. Final QA at DiagnoCine R&D Center, Totowa, NJ, USA.

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Ultrapure Type 1 Water

18.2 MΩ·cm — eliminates ionic contaminants.

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ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending, Certificate of Analysis for every lot.

Endotoxin — USP <85> BET

LAL assay < 0.05 EU/mL per lot.

Particulate — USP <788> Method 2

≤25/mL (≥10 µm), ≤3/mL (≥25 µm).

Osmolality — USP <785>

Target: 240.00–345.00 mOsm/kg H₂O.

Documentation & CoA

Full CoA with raw-material traceability available on request.

Certificate of Analysis: Request for any DCP-WMBM-B1X lot at support@diagnocine.com.

Product Comparison

How DCP-WMBM-B1X compares

FluxMPS™ DCP-WMBM-B1X vs. conventional 0.22 µm–filtered Waymouth formulations.

Parameter	DCP-WMBM-B1X (FluxMPS™)	Conventional Waymouth Medium (0.22 µm filtered)	Standard DMEM/RPMI (0.22 µm filtered)
Waymouth Medium MB 752/1 without Sodium Bicarbonate — CO₂-independent hybridoma and fibroblast culture	check_circle Yes	cancel No	cancel No
Final filtration pore size	0.04 µm	0.22 µm	0.22 µm
Number of filtration stages	4 (Quadruple)	1	1
Mycoplasma barrier	check_circle Yes (0.04 µm)	cancel No	cancel No
Endotoxin specification	< 0.05 EU/mL	NMT 1 EU/mL	NMT 1 EU/mL
USP <788> particulate tested	check_circle Yes	cancel No	cancel No
Water quality	Type 1, 18.2 MΩ·cm	Purified water	Purified water
Manufacturing QMS	ISO 13485:2016	ISO 9001 or none	ISO 9001 or none
Microfluidic channel compatible	check_circle MPS-grade	cancel Risk of clogging	cancel Risk of clogging

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-WMBM-B1X — Waymouth Medium MB 752/1.

DCP-WMBM-B1X is processed through a Quadruple-stage filtration system reaching 0.04 µm final pore size. Waymouth Medium MB 752/1 with 0.04 µm filtration delivers sub-mycoplasma purity for specialized OoC models requiring this medium formulation.

FluxMPS™ uses four sequential filters — 0.1 µm pre-filtration I, 0.04 µm pre-filtration II (mycoplasma barrier), 0.1 µm sterile-filtration I, and 0.04 µm final polish — resulting in approximately 5× fewer particles and confirmed mycoplasma retention at every production stage.

Sodium bicarbonate is removed for CO₂-independent culture via HEPES (15–25 mM recommended) or other buffer. Useful for Waymouth medium applications in atmospheric incubators, open-top chips, and any protocol where CO₂ control is impractical while maintaining the full 5 g/L glucose and glutathione profile.

CO₂-independent; supplement with HEPES (15–25 mM) for pH buffering.

Yes. Add FBS (5–10%), serum-free supplements, growth factors, or antibiotics as required. Contact support@diagnocine.com for custom co-formulation.

FluxMPS™ DCP-WMBM-B1X is produced to meet < 0.05 EU/mL by LAL assay (USP <85>). For primary cells, endotoxin activates TLR4 altering cell phenotype and differentiation outcomes.

Yes. Full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma, particulate count (USP <788> Method 2), and raw-material traceability. Request at support@diagnocine.com.

Scientific References

Supporting literature

Key publications supporting Waymouth Medium MB 752/1 in specialized cell culture and OoC applications.

Waymouth C. Rapid proliferation of sublines of NCTC clone 929 (strain L) mouse cells in a simple chemically defined medium (MB 752/1). J Natl Cancer Inst. 1959;22:1003–1017. doi:10.1083/jcb.1.3.273
Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

FluxMPS™ Waymouth Medium w/o Sodium Bicarbonate: 1X Liquid