Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
World’s cleanest buffer for all cell and molecular biology experiments
Description:
DEPC Treated PBS (Phosphate-Buffered Saline) is a high-purity buffer solution specifically processed to be free of RNase and DNase activity. It is ideal for sensitive molecular biology applications where the integrity of RNA and DNA must be preserved, such as RNA extraction, RT-PCR, in vitro transcription, and other enzymatic reactions.
Applications:
RNA and DNA isolation and purification
Sample preparation for RT-PCR, qPCR, and in vitro transcription
Protein research and immunoassays where nuclease-free conditions are required
Advantages:
RNase- and DNase-Free: Treated with diethyl pyrocarbonate (DEPC), a reagent that inactivates RNases and DNases by covalently modifying their active sites, ensuring reliable protection of nucleic acids from degradation
Sterile and Ready-to-Use: The solution is filtered through a 0.04-micron filter and autoclaved after DEPC treatment to ensure sterility and complete inactivation of residual DEPC, making it immediately suitable for critical experiments
Physiological Buffer: Maintains a pH of 7.0–7.4, mimicking the osmolarity and ion concentrations of the human body, making it compatible with a wide range of cell culture and molecular biology protocols
Versatile Applications: Suitable for use as a wash buffer, storage buffer, sample dilution medium, and for resuspension of cells or nucleic acids in workflows where nuclease contamination must be avoided
Composition:
Ingredients
Concentration
Sodium Chloride (NaCl)
137 mM
Potassium Chloride (KCl)
2.7 mM
Sodium Phosphate Dibasic (Na₂HPO₄)
10.1 mM
Potassium Phosphate Monobasic (KH₂PO₄)
1.76 mM
Water (DEPC-treated, nuclease-free)
To volume
Quality Control:
Appearance: Colorless, clear solution
pH: 7.4
Storage and Shelf Life:
Store at 4 °C.
Shelf life is 24 months.
Use before expiry date given on the product label.
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
