UIBC Assay kit LS (Bathophenanthroline, 200 tests)
UIBC Assay kit LS <Bathophenanthroline, 200 tests>
Cat No: FNK-UIB02AE
Size: 1 kit
- Microassay UIBC (Unsaturated Iron Binding Capacity) can quickly quantify serum / plasma UIBC (Unsaturated Iron Binding Capacity) with a microplate reader (96-well well).
- Since this assay principle is not an immuno-antibody method but a chelate quantification method, it does not depend on the species of the sample.
- Pretreatment is not required when using serum, plasma, or urine as samples.
- Multi-point calibration is not required because the calibration is performed using the standard sample and blank sample included in the kit.
- Since it does not contain poisonous and deleterious substances, it has excellent safety during handling and disposal.
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* It can be applied to the measurement of TIBC (total iron binding capacity) when used in combination with our product iron kit .
- * This product is a research kit. Do not use for diagnostic purposes.
Measurement significance
All iron in serum is bound to transferrin, an iron transport protein. About one-third of transferrin is bound to iron and the rest is present as free transferrin, which is not bound to iron. Total iron-binding capacity (TIBC) represents all transferrins in serum as iron-binding capacity, and unsaturated iron-binding capacity (UIBC) also represents transferrin that is not bound to iron. The relationship between TIBC and UIBC is “TIBC = UIBC + serum iron”. TIBC fluctuates due to abnormal iron metabolism, blood disorders, liver disorders, tumors, inflammation, etc.
UIBC is known to be high in iron deficiency and low in infectious diseases, inflammation, malignancies, nephrotic syndrome, and hypoprotein symptoms.
Measurement principle
This method observes the color development of the visible part due to the formation of a chelate complex between bassofenantrolin and iron, and determines the unsaturated iron binding capacity (UIBC).
When a buffer containing a known excess amount of iron is added to the sample, unsaturated transferrin in the sample binds to iron in the buffer to form saturated transferrin.
Next, when a color test solution (basofenantrolin / reducing agent) is added, the residual iron that did not bind to transferrin turns red.
UIBC can be obtained by observing the absorbance at this time with 546 nm as the main wavelength and 600 nm as the secondary wavelength, and subtracting the residual iron amount from the known excess amount of iron.
