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Trypsin 0.1% Solution in Dulbecco’s Phosphate Buffered Saline (1X)

Product#: DCP-CD001
$18.22
DCP-CD001
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
FluxMPS™ Cell Dissociation Reagent
ISO 13485 Certified Manufacturing

FluxMPS™ Trypsin 0.1% Solution in Dulbecco's Phosphate Buffered Saline (1X)

Microfluidics Suitable, dual-stage ultra-filtered (0.1 µm + 0.04 µm) 0.1% trypsin solution prepared in 1X Dulbecco's Phosphate Buffered Saline, engineered for gentle, reproducible dissociation of adherent cells in microfluidic, organ-on-a-chip (OoC), and standard cell culture workflows.

  • Trypsin (CAS 9002-07-7, EC 3.4.21.4), a 223-residue serine protease, supplied as a 0.1% w/v solution in 1X Dulbecco's Phosphate Buffered Saline
  • Dual-stage filtration: 0.1 µm prefiltration followed by a 0.04 µm final filter for microchannel-safe clarity
  • Colorless, clear solution; pH 7.00 - 7.60; osmolality 275 - 315 mOsm/kg H2O
  • Sterility confirmed by 14-day USP <71> incubation with no bacterial or fungal growth observed
  • Lot-released against a functional Cell Dissociation Test, not just a compositional spec
  • Validated for dissociating adherent cell monolayers, routine passaging, and primary tissue dissociation
  • Manufactured under an ISO 13485:2016 quality management system; final packaging, QC, and customization performed at Diagnocine, Totowa, NJ
  • Custom pH, concentration, and EDTA-supplemented formulations available on request
SKU: DCP-CD001 · Cell Dissociation Reagent UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Trypsin 0.1% Solution in 1X DPBS — Liquid
  • Active EnzymeTrypsin, 0.1% w/v
  • Buffer SystemDulbecco's Phosphate Buffered Saline (1X)
  • Molecular Weight23.4 kDa
  • pH (USP <791>)7.00 - 7.60
  • Osmolality (USP <785>)275 - 315 mOsm/kg H2O
  • Sterility (USP <71>)No growth, 14-day incubation
  • Filtration0.1 µm ×1 + 0.04 µm ×1 (dual-stage)
  • Storage-20°C, non-self-defrosting freezer
  • Shelf Life24 months from date of manufacture
  • ShippingDry ice
ISO 13485:2016 USP <71> <785> <791> RUO
Why FluxMPS™

Engineered where standard dissociation reagents fall short

Conventional 0.22 µm filtered trypsin solutions leave sub-micron particulates and mycoplasma-scale contaminants in the reagent, which can accumulate in microfluidic channels, foul sensors, and confound downstream imaging. FluxMPS™ addresses each failure mode directly.

filter_alt

Microchannel-safe purity

A 0.1 µm prefilter followed by a 0.04 µm final filter removes particulate well below the size that clogs microfluidic channels and valves.

target

Consistent, lot-qualified activity

Every lot is released against a functional Cell Dissociation Test, not a compositional assay alone, so dissociation performance is verified before shipment.

water_drop

Defined buffer system

Prepared in 1X Dulbecco's Phosphate Buffered Saline at a controlled pH (7.00 - 7.60) and osmolality (275 - 315 mOsm/kg H2O) for predictable, reproducible dissociation.

visibility

Low background for imaging

Reduced particulate carryover supports cleaner downstream flow cytometry and live-cell microscopy after cell recovery.

science

Validated for sensitive cell types

Suitable for primary tissue dissociation and routine passaging of established and stem-cell-derived lines where dissociation gentleness matters.

tune

Customization on demand

Concentration, pH, and EDTA supplementation can be tailored to specific cell lines and protocols — contact support@diagnocine.com.

Purity Architecture

Dual-stage filtration system

This trypsin solution is processed through a validated two-pass filtration train reaching a 0.04 µm final pore size, engineered as a dedicated prefilter and final-filter pair rather than a simple descending cascade.

  1. 0.1 µm

    Prefiltration

    Removes large particulate and protein aggregates, protecting the downstream 0.04 µm final filter cartridge.

  2. 0.04 µm

    Final filtration — Polish

    Retains sub-micron particulates and microaggregates that pass through a conventional 0.22 µm filter.

Filtration facts

Two dedicated filtration passes reach a final cut-off well below the industry-standard 0.22 µm, without altering enzymatic activity.

0.04 µmFinal pore size
2Filtration passes
Sterility is confirmed by absence of bacterial or fungal growth after 14 days of incubation per USP <71>. Mycoplasma control is achieved through 0.1 µm mycoplasma-retentive filtration (not tested per lot); mycoplasma organisms typically range 0.2-0.3 µm in diameter.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
Applications

Where this dissociation reagent is used

From routine passaging to seeding microphysiological systems, gentle and reproducible cell recovery is a prerequisite for downstream assay quality.

Automated Bioreactors & Robotics

Next-Generation System Uptime

For automated cell-harvesting platforms and robotic liquid handlers, an optional 0.01 µm (10 nm) ultra-filtered MPS Grade variant of this reagent is available, minimizing particulate load on valves, sensors, and microfluidic manifolds during continuous operation.

  • Total Particulate Exclusion — supports long-run automated harvesting cycles
  • Valve & Sensor Protection — reduces fouling in robotic dispensing systems
  • Extended Perfusion Stability — consistent reagent quality across large batch runs

Inquiry Required: The 0.01 µm MPS Grade variant is produced on request — contact support@diagnocine.com to discuss automated system requirements.

Microfluidics

Cell Harvesting for Microphysiological Systems (MPS) & Chip Seeding

Low-particulate dissociation for seeding cells into microfluidic devices without channel or valve fouling.

OoCToCBoCLoCMPS
Cancer Biology

Passaging of Cancer Cell Lines

Gentle, reproducible dissociation for maintaining and subculturing adherent cancer cell lines.

MCF-7MDA-MB-231HeLaA549
Stem Cell Biology

iPSC & Primary Cell Harvesting

Supports dissociation protocols for iPSC-derived and primary cell models requiring gentle handling.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cell Dissociation

Used for detachment and passaging of endothelial and primary hepatic cell cultures.

HUVECsHAECsPrimary hepatocytes
Sample Preparation

Cell Harvesting for Metabolic Flux Assays

Used to recover cells prior to seeding into metabolic flux and tracer-based assay platforms.

13C tracingNMR metabolomics
Live-Cell Imaging

Cell Recovery for Microscopy & Flow Cytometry

Low-particulate reagent supports cleaner downstream flow cytometry and live-cell imaging after harvesting.

Flow cytometryConfocal reseeding
Recommended dissociation protocol.
  1. Aspirate spent medium from the culture vessel.
  2. Wash the monolayer with a calcium- and magnesium-free balanced salt solution, rocking the flask 1-2 minutes; discard the wash.
  3. Add sufficient Trypsin solution to cover the monolayer.
  4. Rock the flask to ensure even coverage of the cell sheet.
  5. Incubate at 37°C for 2-3 minutes, monitoring under an inverted microscope until cells round up and detach; gentle tapping may assist difficult-to-remove lines.
  6. Add serum-containing complete medium to inhibit tryptic activity.
  7. Disperse cells into a single-cell suspension by repeated pipetting.
  8. Count and subculture the cells.

Concentration and exposure time should be optimized empirically for each cell line; dissociation time depends on cell type, density, trypsin potency, serum concentration, and time since last subculture.

Technical Specifications

Full parameter set

All parameters below are release specifications verified prior to shipment.

Physical & Chemical Parameters
Parameter Specification
Formulation Trypsin, 0.1% w/v in 1X Dulbecco's Phosphate Buffered Saline
Appearance Colorless, clear solution
pH USP <791> 7.00 - 7.60
Osmolality USP <785> 275 - 315 mOsm/kg H2O
Active Enzyme Trypsin, 0.1% w/v (1,000 mg/L)
Molecular Weight 23.4 kDa
EC Number 3.4.21.4
Sterility, Purity & Safety Parameters
Parameter Specification
Sterility USP <71> No bacterial or fungal growth, 14-day incubation
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Cultural Response Cell Dissociation Test
Manufacturing Std. ISO 13485:2016
Storage, Handling & Logistics
Parameter Specification
Storage Temperature -20°C, non-self-defrosting freezer
After Thawing Stable ~2 weeks at 2-8°C; avoid repeated freeze-thaw
Shelf Life 24 months from date of manufacture; use before label expiry
Shipping Condition Dry ice
Available Pack Sizes 100 mL, 500 mL
Raw Materials & Regulatory Traceability
Parameter Specification
CAS Number 9002-07-7
Manufacturing QMS ISO 13485:2016
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory Alignment ISO 13485:2016 certified manufacturing
Production Method Custom formulation, final QC and packaging at Diagnocine, Totowa, NJ
Intended Use Research Use Only (RUO)
Formulation

Product composition

Component identity, concentration, and quality attributes for this dissociation reagent, released per lot.

Component CAS / EC Number Concentration
Trypsin CAS 9002-07-7 / EC 3.4.21.4 1,000 mg/L (0.1% w/v)
Component CAS Number Concentration
Dulbecco's Phosphate Buffered Saline (1X) — Formulated to pH 7.00 - 7.60, 275 - 315 mOsm/kg H2O
Attribute Method Result / Specification
Appearance Visual Colorless, clear solution
pH USP <791> 7.00 - 7.60
Osmolality USP <785> 275 - 315 mOsm/kg H2O
Sterility USP <71> No growth, 14-day incubation
Cultural Response Functional assay Cell Dissociation Test
Molecular Weight — 23.4 kDa
Custom concentration, pH, and EDTA supplementation are available on request — contact support@diagnocine.com.
Quality Assurance

Manufacturing & compliance

This product is manufactured under ISO 13485-certified facilities (Suppliers of Diagnocine Precision). All final packaging, quality assurance, and testing are performed at the Diagnocine R&D and Quality Testing Center. All specific customization requests and assembly are performed at Diagnocine Precision in Totowa, New Jersey, USA.

verified

ISO 13485:2016 QMS

Manufactured under a certified quality management system throughout production and release.

filter_alt

Dual-stage filtration

0.1 µm prefiltration + 0.04 µm final filtration for microchannel-safe clarity.

fact_check

Lot-released Cell Dissociation Testing

Each lot is functionally verified for dissociation performance before release.

location_on

Finished in Totowa, NJ

Final QC, packaging, and customization performed at Diagnocine Precision, Totowa, New Jersey, USA.

Sterility USP <71>

No bacterial or fungal growth observed after 14 days of incubation.

Mycoplasma Control

0.1 µm mycoplasma-retentive filtration applied during processing (not tested per lot).

pH & Osmolality USP <791> / <785>

pH 7.00 - 7.60; osmolality 275 - 315 mOsm/kg H2O, verified per lot.

Documentation / CoA

Certificate of Analysis available for every lot, including QC results and expiry.

A Certificate of Analysis is available on request — contact support@diagnocine.com.
Product Comparison

How DCP-CD001 compares

A parameter-by-parameter comparison against conventional 0.22 µm filtered trypsin reagents.

Parameter DCP-CD001 (FluxMPS™) Conventional Trypsin Solution Standard Alternative
Grade Microfluidics Suitable (0.04 µm final filtration) Standard grade (0.22 µm filtered) Standard grade (0.22 µm filtered)
Formulation 0.1% trypsin in 1X DPBS, lot-released Cell Dissociation Test 0.1%-0.25% trypsin, variable QC Formulation varies by supplier
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 2 (0.1 µm + 0.04 µm) 1 (0.22 µm) 1 (0.22 µm)
Mycoplasma barrier filtration check_circle 0.1 µm mycoplasma-retentive cancel Not specified cancel Not specified
Endotoxin (release specification) Not specified Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
Manufacturing QMS check_circle ISO 13485:2016 Varies by supplier Varies by supplier
Microfluidic channel compatibility check_circle Validated for microchannel use Not specified Not specified
Custom formulation available check_circle pH, concentration, EDTA on request cancel Standard formulations only cancel Standard formulations only

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about using this trypsin solution in microfluidic and standard cell culture workflows.

Yes. The dual-stage 0.1 µm + 0.04 µm filtration reduces particulate load below the level that typically fouls microfluidic channels, valves, and sensors, making this reagent suitable for harvesting and reseeding cells in OoC, ToC, and MPS platforms.
A 0.1 µm prefilter removes large particulate and protein aggregates before a dedicated 0.04 µm final filter retains sub-micron particulates and microaggregates that pass through conventional 0.22 µm filters, without affecting enzymatic activity.
This formulation is a plain trypsin solution without added EDTA, allowing users to select chelation support independently based on cell-type sensitivity. EDTA-supplemented (Trypsin-EDTA) formulations, along with custom concentration and pH, are available on request — contact support@diagnocine.com.
No. Dissociation is a brief 2-3 minute incubation at 37°C, monitored under an inverted microscope until cells round up and detach; a standard incubator or heated surface is sufficient and CO2 is not required for this short enzymatic step.
Yes. Serum-containing complete medium is added after dissociation to inhibit tryptic activity and protect cells. If serum or other protein-containing additions are filtered before use, a 0.2 µm low-protein-binding PES or PVDF membrane is recommended; 0.04 µm filters are not suitable for serum, as they strip essential lipoprotein and immunoglobulin fractions.
Each lot is tested with a Cell Dissociation Test to confirm functional enzymatic performance, in addition to sterility (USP <71>), pH (USP <791>), and osmolality (USP <785>) release testing.
Yes. A Certificate of Analysis is available for every lot, including lot number, expiry, pH, osmolality, sterility, and Cell Dissociation Test results. Contact support@diagnocine.com to request a CoA.
Scientific References

Supporting literature

Curated references on trypsin dissociation, microfluidic cell handling, and mycoplasma control relevant to this reagent.

  1. Bard J, et al. Trypsinization and cell dissociation methods for adherent cell culture. doi:10.1002/9780470147407
  2. Huh D, Hamilton GA, Ingber DE. From 3D cell culture to organs-on-chips. Trends Cell Biol. doi:10.1016/j.tcb.2011.09.005
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. doi:10.1038/nbt.2989
  4. Rudnicki MA, et al. Enzymatic dissociation methods for primary tissue. Methods Mol Biol. doi:10.1007/978-1-4939-6472-7
  5. Rappaport C. Trypsin and cell dissociation. In Vitro. doi:10.1007/BF02618366
  6. Freshney RI. Culture of Animal Cells: A Manual of Basic Technique. doi:10.1002/9780470649367
  7. Uhrig M, et al. Cell harvesting methods for iPSC-derived models. Stem Cell Reports. doi:10.1016/j.stemcr.2016.01.001
  8. Rottem S, Barile MF. Beware of mycoplasmas. Trends Biotechnol. doi:10.1016/0167-7799(93)90113-6
  9. van Meer BJ, et al. Small molecule absorption by PDMS in the context of drug response bioassays. Biochem Biophys Res Commun. doi:10.1016/j.bbrc.2016.11.062

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