Tris NP-40 Buffer [1X]
Cat No.: DCP-TNP1X
Size: 500 mL & 1 L
Non-Sterile; Ultrapure, Filtered 0.1-micron membrane Once and 0.04-micron membrane Once.
Description
Tris NP-40 buffer is a widely used lysis buffer in molecular biology and biochemistry for the extraction of proteins from cells. It combines Tris (tris(hydroxymethyl)aminomethane), a buffering agent, with NP-40, a nonionic detergent, to lyse cells and solubilize proteins while maintaining their native state. This buffer is particularly useful for applications such as immunoprecipitation, Western blotting, and ELISA. NP-40 disrupts the lipid bilayer of cell membranes by integrating into the lipid bilayer and disrupting hydrophobic interactions, leading to cell lysis and the release of cellular contents. The detergent forms micelles around hydrophobic regions of membrane proteins, keeping them in solution and preventing aggregation.
Applications
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Cell Lysis: Effective for lysing both adherent and suspension-cultured mammalian cells to release cytoplasmic and membrane-bound proteins.
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Protein Extraction: Suitable for extracting proteins under mild, non-denaturing conditions, preserving their native structure and function.
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Immunoprecipitation: Used as a wash buffer in immunoprecipitation reactions to minimize non-specific binding and improve the specificity of antibody-antigen interactions.
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Western Blotting and ELISA: Provides a stable environment for protein extraction, making it ideal for downstream applications like Western blotting and ELISA.
Advantages:
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The mild nature of NP-40 ensures that protein functions are retained with minimal disruption, which is crucial for studies involving protein interactions.
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NP-40 is a nonionic detergent, which means it is less harsh than ionic detergents like SDS.
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The buffer is capable of solubilizing cytoplasmic and membrane-bound proteins, making it useful for whole-cell extracts.
Composition:
| Ingredients |
Concentration |
| Tris-base |
50 mM |
| Sodium Chloride |
150 mM |
| EDTA |
5 mM |
| Nonidet P-40 |
1% |
Quality Control:
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Appearance: Clear, Colorless Liquid.
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pH: 8.0
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Sterility: Filtered 0.1-micron membrane Once and 0.04-micron membrane Once.
Storage and Shelf Life:
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Storage: 4 ℃
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Shelf life: 2 years
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
