Taq PCR Premix with Dyes (10x50 tests)

Cat.No: FNK-02-102-5

Size: 10x50 Tests

Storage: -20℃ Repeated freezing and thawing may decrease enzyme activity. If you store this product at 4℃, please use it within 3 months. 

Quality Assurance: Greater than 95% purity as determined by SDS-PAGE (CBB staining). The absence of endonuclease and exonucleases was confirmed.
 

Product Description

Taq PCR Premix with Dyes is an optimized ready-to-use solution containing Taq DNA Polymerase, dNTPs, MgCl2, KCl and stabilizers. It is ideally suited to routine PCR applications from templates including pure DNA solutions bacterial colonies and cDNA products.

Composition

Taq PCR Premix with Dyes (x2) 10 mM Tris-HCl, 50 mM MgCl2, 0.2 mM dNTPs, 5 % Glycerol, 0.08 % NP-40, 0.05 % Tween-20, 25 units/ml Taq DNA Polymerase, pH 8.6 @ 25℃, 0.0005 % BPB, 0.001% XC.
 

PCR Product

PCR products have one A added at the 3'-terminus. Thus, the PCR product can be used directly for cloning into a T-vector. Additionally, it is possible to clone the product in blunt-end vectors after blunting and phosphorylation of the end. 

Application

1. PCR

2. Primer Extension

3. Colony PCR
  

Data

Please Note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTICS PROCEDURES. NOT FOR MILITARY USE. 

Related Products

SKU#	Description	Link
FNK-02-102	Taq PCR Premix with Dyes (2x50 tests)	GO!