THUNDERBIRD SYBR qPCR Mix (100 Rxn)
THUNDERBIRD SYBR qPCR Mix (100 Rxn)
A highly efficient 2× Master Mix from TOYOBO for real-time PCR using SYBR® Green I dye — ready to use, with 50× ROX reference dye supplied separately — for high specificity, robust amplification, and a broad dynamic range.
Cold-chain shipping required. This product has ICE or DRY ICE shipping requirements — do not select Ground Shipping.
Tip: THUNDERBIRD is optimized for PCR amplicon size less than 500 bp and routine PCR purposes. Do not use it for PCR to amplify targets larger than 500 base pairs.
Description
THUNDERBIRD® SYBR® qPCR Mix (Cat. No. TYB-QPS-201T) from TOYOBO is a highly efficient 2× Master Mix designed for real-time PCR using SYBR® Green I dye. This ready-to-use premix includes all essential components except for ROX reference dye and primers (50× ROX reference dye is supplied separately), streamlining reaction setup and improving the reproducibility of experiments. The formulation is optimized for high specificity, robust amplification, and broad dynamic range, making it ideal for gene expression analysis and quantification in research applications.
Key Features
Enhanced detection of low-copy targets and improved discrimination of specific amplification due to hot start technology with anti-Taq DNA polymerase antibodies.
Incorporates a novel PCR enhancer (patent pending) that reduces variability in PCR efficiency between targets, ensuring consistent results across assays.
Effective amplification enables reliable quantification across a wide range of template concentrations.
Suitable for most real-time PCR platforms, including block and glass capillary types. The supplied 50× ROX reference dye allows adaptation to cyclers requiring a passive reference dye.
Hot start mechanism minimizes non-specific amplification and primer-dimer formation, improving assay specificity.
The premix format simplifies reaction assembly and enhances reproducibility between runs.
All components are stable at -20 °C. The master mix can also be stored at 2–8 °C, protected from light, for up to 3 months.
Kit Components
| Solution | Volume |
|---|---|
| THUNDERBIRD™ SYBR qPCR Mix | 1 ml × 1 tube |
| 50× ROX reference dye | 50 µl × 1 tube |
Storage Conditions
Application Data
Example 1. Comparison of the sensitivity and efficiency of the SYBR® Green I assay
β-actin mRNA was detected with serially diluted cDNA from HeLa cell total RNA. THUNDERBIRD™ SYBR® qPCR Mix [Code No. TYB-QPS-201] showed greater sensitivity and efficiency than other kits (companies A and B).
Example 2. Comparison of the PCR efficiency
Norovirus G1 and human GAPDH genes were detected using serially diluted cDNA samples by SYBR® Green I and TaqMan real-time PCR. THUNDERBIRD™ qPCR Master Mix showed greater efficiency than other reagents.
Example 3. Verification of the measurement accuracy
Human GAPDH genes were detected using serially 20.5-fold diluted cDNA synthesised from HeLa cell total RNA by SYBR® Green I assay. THUNDERBIRD™ qPCR Master Mix successfully detected the differences between dilutions.
