THUNDERBIRD® Probe qPCR Mix (Cat. No. TYB-QPS-101T) from TOYOBO is a highly efficient 2× Master Mix designed for real-time PCR using TaqMan® probes and other sequence-specific fluorescent probes. This ready-to-use premix contains all essential components except for ROX reference dye, probe, and primers (50× ROX reference dye is supplied separately), streamlining reaction setup and improving experimental reproducibility. The formulation is optimized for sensitive, specific, and reproducible detection across a broad dynamic range, making it ideal for gene expression analysis, quantification, and multiplex assays in research applications

Key Features

• High Sensitivity and Specificity: Delivers robust and precise amplification with high efficiency, enabling reliable detection of low-copy targets and accurate quantification.
• Broad Dynamic Range: Supports quantitative analysis across a wide range of template concentrations, from low to high abundance targets.
• Optimized for Multiplexing: Enhanced formulation allows efficient and simultaneous detection of multiple targets in a single reaction, even in the presence of PCR inhibitors.
• Fast PCR Compatibility: Enables rapid cycling protocols with extension times as short as 10 seconds, reducing overall assay time without compromising performance.
• Inhibitor Resistance: Maintains high amplification efficiency even when PCR inhibitors are present, supporting use with crude or challenging sample types.
• Stable Reaction Mix: Unique buffer composition improves the stability of prepared reaction mixtures, maintaining consistent Ct values even after 48 hours at room temperature.
• dUTP Carryover Prevention Compatible: Can be used with Uracil-DNA Glycosylase (UNG, sold separately) to prevent false positives from carryover contamination.
• Instrument Compatibility: Suitable for use with a wide variety of real-time PCR platforms and compatible with common reverse transcription reagents for one-step or two-step RT-qPCR workflows.
• Convenient Storage: All components are stable at -20°C.

Kit Components:

  
Storage Conditions

• THUNDERBIRD® Probe qPCR Mix: Store at -20°C.
• 50× ROX Reference Dye: Store at -20°C or at 2–8°C protected from light.
• Premixed Master Mix with ROX: If prepared, can be stored at 2–8°C protected from light for up to 3 months; for longer-term storage, keep at -20°C.