THUNDERBIRD Probe qPCR Master Mix

Product#: QPS-101
$1.00

Size:

  • Trial
  • 1PK
  • 5PK
Availability:
Ships in 24 hours

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THUNDERBIRD® Probe qPCR Master Mix

Cat.No. List below
 

Product Name

Cat. No.

Size

Storage

Price USD

THUNDERBIRD Probe qPCR Master Mix

QPS-101_Trial

1 ml

-20oC

1.00

THUNDERBIRD Probe qPCR Master Mix

QPS-101_1PK

1.67 ml x 3

173.00

THUNDERBIRD Probe qPCR Master Mix

QPS-101_5PK

(1.67 ml x3) x 5

791.00


Description

THUNDERBIRD® Probe qPCR Mix is a Taq DNA polymerase-based 2x Master Mix for highly efficient real-time PCR using TaqMan® probes. The master mix contains all required components, except for ROX reference dye, probe and primers. 50x ROX reference dye is individually supplied with this kit. The master mix facilitates reaction setup, and improves the reproducibility of experimental data.

This product is an improved version of  Realtime PCR Master Mix (Code No. QPK-101) . In particular, reaction specificity and PCR efficiency is enhanced.

QPS-101_Description.jpg

 
 
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Features
  • The master mix contains anti-Taq DNA polymerase antibodies for hot start technology. The specificity for the detection of low-copy targets is enhanced.
  • The dispersion of PCR efficiency between targets is reduced by a new PCR enhancer (Patent pending) in the master mix.    
  • High specificity and robust enzyme enable the detection of a broad dynamic range.
  • The reagent is applicable to most real-time cyclers (i.e. Block type and glass capillary type) including ABI, Bio-Rad, Roche, and TaKaRa. The 50x ROX reference dye in the kit can be applied to real-time cyclers that require a passive reference dye.
 Specifications
  • Hot Start
  • Probe
  • 2x Reaction mix
  • 20µl total reaction volume
  • Store at -20oC
  • 50x ROX reference dye
Components
  •  QPS-101T
    • THUNDERBIRD® Probe qPCR Mix    1 ml × 1
    • 50x ROX reference dye    50 µl × 1
  •  
  • QPS-101
    • THUNDERBIRD® Probe qPCR Mix    1.67 ml × 3
    • 50x ROX reference dye    250 µl × 1
  •  
  • QPS-101X5
    • THUNDERBIRD® Probe qPCR Mix    (1.67 ml × 3) x 5
    • 50x ROX reference dye    250 µl × 5

Compatible real-time instruments
QPS-101_Compatible.jpg
 

Application data

Example 1. Comparison of the sensitivity and efficiency of the SYBR® Green I assay
β-actin mRNA was detected with serially diluted cDNA from HeLa cell total RNA. THUNDERBIRD® SYBR® qPCR Mix [Code No. QPS-201] showed greater sensitivity and efficiency than other kits (companies A and B). 

QPS- 101_example1_1.png     QPS- 101_example1_2.png



QPS- 101_example1_3.png

Example 2. Comparison of the PCR efficiency
Norovirus G1 and human GAPDH genes were detected using serially diluted cDNA samples by SYBR® Green I and TaqMan real-time PCR. THUNDERBIRD® qPCR Master Mix showed greater efficiency than other reagents.

QPS-101_example2_1.png 
Detection of Norovirus G1 gene by SYBR® Green I assay. [Roche Diagnostics LightCycler 1.1]

QPS-101_example2_2.png 
Detection of GAPDH gene by TaqMan® assay. [Roche Diagnostics LightCycler 1.1]

Example 3. Verification of the measurement accuracy
Human GAPDH genes were detected using serially 20.5 fold diluted cDNA synthesised from HeLa cell total RNA by SYBR® Green I assay. THUNDERBIRD® qPCR Master Mix successfully detected the differences between dilutions.

QPS-101_example3.png 
Detection of human GAPDH gene by SYBR® GREEN I assay. [Applied Biosystems 7900HT]

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