TBST Blocking Buffer with Bovine Serum Albumin (3%)
Cat No.: DCP-BSATBST3X
Size: 100 mL, 500 mL & 1000 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane TWICE.
Description:
TBST Blocking Buffer with Bovine Serum Albumin (3%) is a specialized reagent used in various immunoassays, such as Western blotting, ELISA, and immunohistochemistry. This buffer is designed to minimize non-specific binding, thereby enhancing the sensitivity and specificity of these assays. Bovine Serum Albumin (BSA) serves as the primary blocking agent. BSA is a single purified protein with a high affinity for proteins, making it effective at saturating excess protein-binding sites on membranes and microplates. This helps reduce non-specific binding, which is crucial for obtaining clear and accurate results in assays. Tris-Buffered Saline with Tween-20 (TBST) provides a stable pH environment and includes Tween-20, a detergent that helps reduce non-specific binding. Tween 20 contributes significantly to the reduction of background noise and enhancement of assay specificity, thereby improving the overall reliability and sensitivity of immunoassays. BSA in TBST is compatible with a wide range of antibodies and detection systems. The use of Tris-buffered saline (TBS) avoids interference with alkaline phosphatase detection systems, which can occur with phosphate-buffered saline (PBS).
Applications
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Western Blotting: Used to block non-specific sites on membranes, ensuring that antibodies bind specifically to target proteins.
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ELISA (Enzyme-Linked Immunosorbent Assay): Reduces background noise, improving the accuracy of antigen-antibody interactions.
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Immunohistochemistry (IHC): Prevents non-specific binding of antibodies to tissue sections, enhancing the clarity of staining.
Advantages:
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High Affinity for Proteins: BSA is a purified single-type protein, which reduces the risk of cross-reactivity and provides a consistent blocking effect.
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Reduced Background Interference: The inclusion of Tween-20 helps minimize background signals, leading to clearer and more reliable results.
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Compatibility with Various Detection Systems: Suitable for a wide range of antibodies and detection systems, making it versatile for different assays.
Composition:
| Ingredients |
Concentration |
| BSA |
3% |
| Tris-Base |
50 mM |
| Sodium Chloride |
150 mM |
| Tween 20 |
0.05% |
Quality Control:
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Appearance: Clear, Colorless Liquid.
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pH: 7.4
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Sterility: Filtered 0.1 micron Twice and 0.04 micron Twice in a sterile environment.
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DNase activity: None was detected after incubation of plasmid DNA and this product for 18 hr at room temperature.
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RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
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Storage: -20 ℃
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Shelf life: 1 year
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
