TBS with 5% Nonfat Soy Milk
FluxMPS™ TBS with 5% Nonfat Soy Milk
An MPS-grade, Tris-buffered saline blocking and dilution buffer formulated with 5% nonfat soy milk for Western blotting and immunoassay workflows. Precisely buffered at pH 7.4 with 20 mM Tris-Base and 150 mM Sodium Chloride, the formulation is quadruple-stage filtered (0.1 µm membrane, Twice, and 0.04 µm membrane, Twice) for ultra-clean, microchannel-safe performance in sensitive detection and microfluidic applications.
- Quadruple-stage filtration: 0.1 µm membrane (Twice) and 0.04 µm membrane (Twice)
- Tris-buffered saline base: 20 mM Tris-Base, 150 mM Sodium Chloride, pH 7.4
- 5% Nonfat Soy Milk blocking agent — casein-free, biotin-free, animal-protein-free
- Prepared with Ultrapure Type 1 water (18.2 MΩ·cm)
- DNase- and RNase-tested: none detected after 18 hr incubation at room temperature
- Manufactured under ISO 13485-certified, CE-approved facilities
- Customizable concentration, pH, and additive content available on request
- pH7.4
- Tris-Base20 mM
- Sodium Chloride150 mM
- Nonfat Soy Milk5%
- ProClin 300 (Preservative)0.05%
- Filtration0.1 µm (Twice) + 0.04 µm (Twice)
- AppearanceClear, Colorless Liquid
- Storage4°C
- Shelf Life1 year
- Sterility TestingDNase & RNase Not Detected
Engineered where standard blocking buffers fall short
Conventional single-pass 0.22 µm-filtered blocking buffers can carry residual particulates, batch-to-batch pH drift, and animal-derived proteins that interfere with sensitive detection chemistries. FluxMPS™ TBS with 5% Nonfat Soy Milk is engineered against each of those failure modes.
Microchannel-safe purity
Quadruple-stage filtration through 0.1 µm membrane (Twice) and 0.04 µm membrane (Twice) removes fine particulates before the buffer reaches your assay or microchannel.
Precise, stable pH
Formulated at pH 7.4 with 20 mM Tris-Base and 150 mM Sodium Chloride to support optimal antibody-antigen interactions and reproducible blot-to-blot performance.
Ultrapure-grade water
Prepared with Ultrapure Type 1 water (18.2 MΩ·cm) as the aqueous base for a low-background, reproducible buffer system.
Low-background blocking
Soymilk-based blocking has been reported to achieve a higher signal-to-noise ratio than nonfat dry milk, with effective blocking possible in as little as 10 seconds in some cases.[1]
Defined, traceable composition
Nonfat Soy Milk, ProClin 300, Tris-Base, and Sodium Chloride are each present at a controlled, stated concentration for consistent performance lot to lot.
Customization on demand
Alternate concentrations, additional chemicals, compounds, proteins, supplements, different pH, or other modifications can be produced on request.
Quadruple-stage filtration system
DCP-DMB05 is sterile-filtered through a 0.1 µm membrane (Twice) followed by a 0.04 µm membrane (Twice) in a sterile environment, sequentially removing progressively finer particulates and bioburden before final fill.
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1
0.1 µm Pre-filtration I
First pass through a 0.1 µm membrane removes large particulates and aggregates, extending the service life of downstream filters.
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2
0.04 µm Pre-filtration II
A first 0.04 µm membrane pass retains finer particulates and bioburden, including organisms in the size range of the smallest mycoplasma, reported at about 0.2 micron.
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3
0.1 µm Sterile-filtration I
A second 0.1 µm membrane pass provides redundant particulate exclusion ahead of final polishing.
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4
0.04 µm Sterile-filtration II — Final Polish
A second 0.04 µm membrane pass in a sterile environment delivers the final polish that helps prevent mycoplasma contamination of the finished buffer.
Performance vs. conventional buffer
Sequential 0.1 µm (Twice) and 0.04 µm (Twice) filtration provides multiple independent purification passes ahead of final fill, in contrast to a single-pass 0.22 µm filtration commonly used for standard blocking buffers.
© Diagnocine® — DCP-DMB05
Where DCP-DMB05 is used
A Tris-buffered, soy-milk-based blocking and dilution buffer for Western blotting, immunoblotting, and related detection workflows, with broader utility across microfluidic and cell-based platforms.
Automated Bioreactors & Robotics
For automated bioreactor and robotic liquid-handling platforms, an optional 0.01 µm (10 nm) ultra-filtered variant of this buffer can be produced on request for applications demanding the lowest achievable particulate load.
- Total Particulate Exclusion for sensitive automated fluid paths
- Valve & Sensor Protection in closed-loop perfusion systems
- Extended Perfusion Stability for long-duration automated runs
Inquiry Required: the 0.01 µm ultra-filtered grade is produced to order — contact support@diagnocine.com to request this configuration.
Micro Physiological System (MPS) & Chip
Ultra-filtered buffer base suitable for use in microphysiological system and chip-based assay setups.
Wash, Dilution & Reconstitution
Serves as a medium for diluting primary and secondary antibodies used in immunoblotting procedures.
iPSC-Derived Model Handling
Tris-buffered saline base compatible with wash and dilution steps used alongside iPSC-derived model workflows.
Endothelial & Primary Cell Perfusion
Ultra-filtered, low-particulate buffer suitable for perfusion-adjacent wash and dilution steps with primary and endothelial cell models.
ELISA, Blotting & Blocking
Blocks non-specific binding sites on the membrane, reducing background noise and improving the signal-to-noise ratio in Western blots; TBS is recommended when using alkaline phosphatase (AP) conjugates since phosphate in PBS can interfere with AP activity.
Microscopy & Optical Sensing
Low-particulate, ultra-filtered formulation suited to buffer needs around imaging and optical sensing workflows.
Product specifications
Measured and declared values for DCP-DMB05, as released by Diagnocine.
| Parameter | Specification |
|---|---|
| Formulation | Tris-Buffered Saline with 5% Nonfat Soy Milk |
| Appearance | Clear, Colorless Liquid |
| pH USP <791> | 7.4 |
| Tris-Base | 20 mM |
| Sodium Chloride | 150 mM |
| Nonfat Soy Milk | 5% |
| ProClin 300 | 0.05% |
| Parameter | Specification |
|---|---|
| Sterility / Filtration | 0.1 µm membrane (Twice) and 0.04 µm membrane (Twice) in a sterile environment |
| DNase Activity | None detected (plasmid DNA, 18 hr, room temperature) |
| RNase Activity | None detected (ribosomal RNA, 18 hr, room temperature) |
| Water Quality | Ultrapure Type 1 Water (18.2 MΩ·cm) |
| Manufacturing Standard ISO 13485 | ISO 13485-Certified Facility |
| Parameter | Specification |
|---|---|
| Storage Temperature | 4°C |
| Shelf Life | 1 year |
| Parameter | Specification |
|---|---|
| Manufacturing QMS | ISO 13485-Certified |
| Regulatory Alignment | CE-Approved Supplier Facility |
| Production Method | Final packaging, QA, and testing at the Diagnocine R&D and Quality Testing Center; customization and assembly at Diagnocine Precision, Totowa, New Jersey, USA |
| Intended Use | Research Use Only (RUO) |
Full composition
Every component of DCP-DMB05 with its stated concentration, as released per lot.
| Component | CAS Number | Concentration |
|---|---|---|
| Nonfat Soy Milk | 5% | |
| ProClin 300 | 0.05% | |
| Tris-Base | 77-86-1 | 20 mM |
| Sodium Chloride | 7647-14-5 | 150 mM |
Manufacturing & compliance
DCP-DMB05 is produced and released under a controlled quality system with defined testing at each stage.
ISO 13485:2016 QMS
Manufactured under ISO 13485-certified and CE-approved supplier facilities.
Ultrapure Type 1 Water
Prepared using Ultrapure Type 1 water (18.2 MΩ·cm) as the aqueous base.
Controlled Fill & Finish
Final packaging, quality assurance, and testing are performed at the Diagnocine R&D and Quality Testing Center.
Micro-Batch Precision
Customization requests and assembly are accomplished at Diagnocine Precision in Totowa, New Jersey, USA.
DNase Activity Testing
None detected after incubation of plasmid DNA with this product for 18 hr at room temperature.
RNase Activity Testing
No RNase activity detected after incubation of ribosomal RNA with this product for 18 hr at room temperature.
Sterility & Filtration Validation
Filtered through a 0.1 µm membrane (Twice) and a 0.04 µm membrane (Twice) in a sterile environment.
Documentation / CoA
A Certificate of Analysis is available on request for lot-specific release data.
How DCP-DMB05 compares
A side-by-side view of DCP-DMB05 against conventional dairy-milk-based TBS blockers and standard PBS-based blocking buffers.
| Parameter | DCP-DMB05 (FluxMPS™) | Conventional 0.22 µm-Filtered TBS + Dry Milk Blocker | Standard PBS-Based Blocking Buffer |
|---|---|---|---|
| Final Filtration Pore Size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of Filtration Stages | 4 | 1 | 1 |
| Animal-Protein-Free Blocking Agent | check_circle | cancel | cancel |
| Casein-Free Formulation | check_circle | cancel | cancel |
| Biotin-Free Formulation | check_circle | cancel | cancel |
| Compatible with Alkaline Phosphatase (AP) Detection | check_circle | check_circle | cancel |
| DNase / RNase Tested | check_circle | cancel | cancel |
| ISO 13485-Certified Manufacturing | check_circle | cancel | cancel |
| Custom Formulation Available | check_circle | cancel | cancel |
Frequently asked questions
Common questions about DCP-DMB05, TBS with 5% Nonfat Soy Milk.
Supporting literature
Citations and curated literature relevant to soymilk-based blocking, Tris-buffered saline chemistry, and microfluidic/organ-on-a-chip applications.
Citations
- Galva, C., Gatto, C., & Milanick, M. (2012). Soymilk: an effective and inexpensive blocking agent for immunoblotting. Analytical Biochemistry, 426(1), 22-23. doi:10.1016/j.ab.2012.03.028
Supporting Literature
- Bhatia, S. N., & Ingber, D. E. (2014). Microfluidic organs-on-chips. Nature Biotechnology, 32(8), 760-772. doi:10.1038/nbt.2989
- Huh, D., Matthews, B. D., Mammoto, A., Montoya-Zavala, M., Hsin, H. Y., & Ingber, D. E. (2010). Reconstituting organ-level lung functions on a chip. Science, 328(5986), 1662-1668. doi:10.1126/science.1188302
- Sackmann, E. K., Fulton, A. L., & Beebe, D. J. (2014). The present and future role of microfluidics in biomedical research. Nature, 507(7491), 181-189. doi:10.1038/nature13118
- Towbin, H., Staehelin, T., & Gordon, J. (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, 76(9), 4350-4354. doi:10.1073/pnas.76.9.4350
- Mahmood, T., & Yang, P. C. (2012). Western blot: technique, theory, and trouble shooting. North American Journal of Medical Sciences, 4(9), 429-434. doi:10.4103/1947-2714.100998
- Good, N. E., Winget, G. D., Winter, W., Connolly, T. N., Izawa, S., & Singh, R. M. (1966). Hydrogen ion buffers for biological research. Biochemistry, 5(2), 467-477. doi:10.1021/bi00866a011
- Sivagnanam, V., & Gijs, M. A. M. (2013). Exploring living multicellular organisms, organs, and tissues using microfluidic systems. Chemical Reviews, 113(5), 3214-3247. doi:10.1021/cr300223v
- Wikswo, J. P. (2014). The relevance and potential roles of microphysiological systems in biology and medicine. Experimental Biology and Medicine, 239(9), 1061-1072. doi:10.1177/1535370214542068
