Soil gDNA Kit (50 Preps)

Product#: BMG-GD2412-01
$90.00
Availability:
Ships in 1-2 Weeks

Soil gDNA Kit (50 Preps)

Cat. No.: BMG-GD2412-01
Size: 50 Preps
 

Description:

A Soil gDNA Kit is a specialized tool designed for extracting high-quality genomic DNA from soil samples.

Applications:
  • PCR and qPCR for detecting and quantifying specific microorganisms or genes.
  • Next-generation sequencing for metagenomic analysis and community profiling.
  • Restriction enzyme digestion for genetic fingerprinting and cloning.
  • Environmental monitoring to assess soil health and detect contaminants.
  • Agricultural research to study plant-microbe interactions and soil microbial communities.
  • Ecological studies to investigate microbial diversity and succession in different ecosystems.
Features:
  • Rapid extraction process (30-40 minutes).
  • High DNA yield (up to 5 μg from 250-500 mg soil).
  • These kits can extract DNA from various soil types, including clay, sandy, peaty, chalky, and loamy soils.
  • Effective removal of PCR inhibitors.
  • Kits can isolate DNA from diverse microorganisms including bacteria (Gram-positive and Gram-negative), archaea, fungi, yeast, and algae.
  • Easy-to-use components (e.g., pre-filled bead beating tubes).
  • Silica membrane technology for DNA purification.
  • No need for organic extractions.
  • The purified DNA is suitable for PCR, restriction enzyme digestion, Southern blotting, and next-generation sequencing.
Components:
  • Lysis buffers
  • Inhibitor removal buffers
  • Binding buffers
  • Wash buffers
  • Elution buffers
  • Spin columns
  • Collection tubes
  • Bead beating tubes with ceramic or glass beads
Reference:
Devi SG, Fathima AA, Radha S, Arunraj R, Curtis WR, Ramya M. A Rapid and Economical Method for Efficient DNA Extraction from Diverse Soils Suitable for Metagenomic Applications. PLoS One. 2015 Jul 13;10(7):e0132441. doi: 10.1371/journal.pone.0132441. PMID: 26167854; PMCID: PMC4500551.
Yeates C, Gillings MR, Davison AD, Altavilla N, Veal DA. Methods for microbial DNA extraction from soil for PCR amplification. Biol Proced Online. 1998 May 14;1:40-47. doi: 10.1251/bpo6. PMID: 12734590; PMCID: PMC140122.

Satisfaction
Quality Rating
Value Rating
Style Rating
X