Sodium Chloride and Sodium Hydroxide Transfer Buffer
Cat No.: DCP-NACLNAOH1X
Size: 500 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
World’s cleanest buffer for all cell and molecular biology experiments
Description:
Our 3 M NaCl / 10 mM NaOH Transfer Buffer is a high-quality solution designed for optimal nucleic acid transfer in molecular biology applications, particularly excelling in RNA transfer for Northern blotting procedures. This carefully formulated buffer combines the ionic strength of 3 M sodium chloride with the mild alkalinity of 10 mM sodium hydroxide, creating an ideal environment for RNA denaturation during the transfer process. The buffer's composition ensures efficient and consistent transfer of RNA molecules from gel to membrane, promoting clear and reliable results in your Northern blot experiments. This transfer buffer is an essential component for researchers aiming to achieve high-quality blots and accurate gene expression analysis. Whether you're conducting routine Northern blots or engaging in advanced RNA studies, our transfer buffer provides the reliability and performance you need for successful nucleic acid transfer.
Applications:
RNA Transfer: This buffer is primarily used for efficient RNA transfer in Northern blotting procedures. The mildly alkaline conditions help denature the RNA as it is deposited onto the membrane during transfer, ensuring optimal binding.
Membrane Compatibility: This transfer buffer is particularly well-suited for use with positively charged nylon membranes. The high salt concentration and alkaline pH promote efficient nucleic acid binding to these membranes.
Capillary Transfer Systems: The buffer is commonly used in downward capillary transfer setups, which are faster than traditional upward transfer methods and result in tighter bands and higher signal intensity.
Advantages:
Efficient RNA denaturation: The mildly alkaline conditions created by 10 mM NaOH help denature RNA molecules during the transfer process, ensuring optimal binding to the membrane.
High salt concentration: The 3 M NaCl provides a high ionic strength environment, which promotes efficient transfer of nucleic acids from gel to membrane.
Optimal for positively charged membranes: This buffer composition is well-suited for use with positively charged nylon membranes, enhancing nucleic acid binding.
Preservation of RNA integrity: The mild alkalinity helps maintain RNA integrity during transfer, which is crucial for downstream applications like hybridization and gene expression analysis
Composition:
Ingredients
Concentration
Sodium Chloride
3 M
Sodium Hydroxide
10 mM
Quality Control:
Appearance: Clear, Colorless Liquid.
pH: 12
Sterility: Filtered 0.1 micron Twice and 0.04 micron once in a sterile environment.
DNase activity: None was detected after incubation of plasmid DNA and this product for 18 hr at room temperature.
RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
Storage: Room temperature
Shelf life: 6 months
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
