SigMax HRP-PBS kit
FluxMPS™ SigMax HRP-PBS Kit
SigMax HRP-PBS Kit is an MPS-grade, phosphate-buffered saline (PBS) based kit purpose-built for horseradish peroxidase (HRP) immunoassay workflows. It pairs sequentially filtered antibody dilution buffers with a dedicated HRP suppression buffer, delivering the defined pH and ultra-clean particulate profile that sensitive colorimetric and immunohistochemistry assays demand. Sequential 0.1 µm and 0.04 µm membrane filtration supports microchannel-safe use in automated and high-throughput immunoassay platforms.
- Sequentially filtered 0.1 µm membrane once and 0.04 µm membrane once for ultra-clean, microchannel-safe purity
- Integrated kit combining SigMax 1Ab-PBS, SigMax 2Ab-PBS, and HRPQuench-PBS (100 mL each) in one unified HRP workflow
- Defined pH 7.4 phosphate-buffered saline (PBS) chemistry, formulated for minimal lot-to-lot variation
- Broad dynamic detection range of 0.5–200 ng/mL for low-abundance analyte quantification
- Ultrapure Type 1 water (18.2 MΩ·cm) formulation base
- Non-Sterile format for research use only
- Manufactured under ISO 13485-certified and CE-approved facilities
- Custom pH, concentration, and additive formulations available on request
- FormatLiquid, Non-Sterile
- pH7.4
- AppearanceClear, Colorless Liquid
- Filtration0.1 µm once + 0.04 µm once
- Detection Range0.5–200 ng/mL
- Kit Volume100 mL per component
- SterilityNon-Sterile
- Storage4°C
- Shelf Life1 year
- Water QualityUltrapure Type 1 (18.2 MΩ·cm)
Engineered where standard PBS reagents fail
Conventional single-pass 0.22 µm filtered PBS reagents can carry residual particulate and inconsistent lot-to-lot antibody performance into HRP-based immunoassays, eroding sensitivity and reproducibility. SigMax HRP-PBS Kit combines advanced antibody incubation buffers with reliable HRP suppression chemistry in one unified, quality-controlled workflow.
Microchannel-safe purity
Sequential 0.1 µm and 0.04 µm membrane filtration reduces particulate carryover for automated and microfluidic immunoassay handling.
Precise, stable pH
Formulated at pH 7.4 with every lot rigorously controlled to minimize lot-to-lot variation and keep results reproducible across experiments.
Ultrapure-grade water
Built on Ultrapure Type 1 water (18.2 MΩ·cm) consistent with USP <85> water-quality expectations for sensitive assays.
Low background for imaging & assays
Supports quantitative cytokine profiling, high-sensitivity Western blotting, and multiplex immunohistochemistry with a broad 0.5–200 ng/mL dynamic range.
Defined, traceable composition
Three named kit components — SigMax 1Ab-PBS, SigMax 2Ab-PBS, and HRPQuench-PBS — manufactured under ISO 13485-certified, CE-approved facilities.
Customization on demand
Alternate concentrations, pH, and added chemicals, compounds, proteins, or supplements are available — contact support@diagnocine.com.
Dual-stage filtration system
SigMax HRP-PBS Kit reagents are sequentially filtered through a 0.1 µm membrane once and a 0.04 µm membrane once, providing an additional polishing stage beyond a single-pass 0.22 µm filtered buffer for microchannel-safe, low-particulate immunoassay handling.
- 1
0.1 µm Pre-filtration
Large particulate and aggregate removal, extending the life of downstream processing and protecting sensitive immunoassay chemistry.
- 2
0.04 µm Final polish filtration
Fine particulate retention for microchannel-safe reagent quality prior to fill, supporting clean, consistent colorimetric signal generation.
Performance vs. conventional buffer
Sequential 0.1 µm and 0.04 µm membrane filtration removes finer particulates than a single 0.22 µm pass, supporting reliable HRP kinetics and reduced assay background.
© Diagnocine® — DCP-HRPPBSKit
Built for HRP-based immunoassay workflows
SigMax HRP-PBS Kit supports quantitative cytokine profiling, high-sensitivity Western blotting, multiplex immunohistochemistry, and longitudinal biomarker tracking, with automation-ready compatibility for major immunoassay platforms.
Automated Bioreactors & Robotics
An optional 0.01 µm (10 nm) ultra-filtered variant of the SigMax HRP-PBS Kit components is available for automated bioreactor and robotics platforms where valve and sensor protection is critical.
- Total Particulate Exclusion for sensitive automated fluidic paths
- Valve & Sensor Protection against fine particulate fouling
- Extended Perfusion Stability for long-duration automated runs
Inquiry Required: the 0.01 µm ultra-filtered variant is available upon request — contact support@diagnocine.com.
Automated Immunoassay Platforms
Optimized for rapid HRP kinetics, enabling fast colorimetric detection with reliable signal generation across manual and automated workflows, fully compatible with major automated immunoassay platforms.
Antibody Dilution & HRP Suppression
SigMax 1Ab-PBS and SigMax 2Ab-PBS support antibody dilution and incubation, while HRPQuench-PBS delivers reliable HRP suppression within the same PBS-based workflow.
iPSC-Derived Model Handling
PBS-based dilution and quench chemistry supports biomarker profiling of iPSC-derived model culture supernatants within HRP-based detection formats.
Endothelial & Primary Cell Perfusion
Defined pH 7.4 PBS chemistry supports downstream immunoassay analysis of endothelial and primary cell perfusate samples.
ELISA, Blotting & IHC
Enables accurate quantitative cytokine profiling in serum and plasma, enhanced detection of low-abundance and phosphoprotein targets by Western blot, and clear discrimination of multiple antigens in multiplex immunohistochemistry.
Longitudinal Biomarker Tracking
Stable HRP performance supports extended studies, including serial clinical sample analysis, on microscopy and optical biosensor detection platforms.
Specifications at a glance
Values below are drawn directly from the SigMax HRP-PBS Kit product documentation.
| Parameter | Specification |
|---|---|
| Formulation / Composition | SigMax 1Ab-PBS, SigMax 2Ab-PBS, and HRPQuench-PBS (100 mL each), PBS-based |
| Appearance | Clear, Colorless Liquid |
| pH USP <791> | 7.4 |
| Parameter | Specification |
|---|---|
| Sterility | Non-Sterile |
| Filtration System | Filtered 0.1 µm membrane once and 0.04 µm membrane once |
| Water Quality | Ultrapure Type 1 water (18.2 MΩ·cm) |
| Manufacturing Standard ISO | ISO 13485-certified, CE-approved facility |
| Parameter | Specification |
|---|---|
| Storage Temperature | 4°C |
| Shelf Life | 1 year |
| Parameter | Specification |
|---|---|
| Raw Material Grade | Ultrapure, sequentially membrane-filtered (0.1 µm once, 0.04 µm once) |
| Manufacturing QMS ISO | ISO 13485-certified |
| Regulatory Alignment | CE-approved |
| Production Site | Final packaging, QA, and testing at the DiagnoCine R&D and Quality Testing Center; customization and assembly at DiagnoCine Precision, Totowa, New Jersey, USA |
| Intended Use | For Research Use Only (RUO) |
Full composition
SigMax HRP-PBS Kit component reagents, each supplied at 100 mL.
| Component | CAS Number | Concentration |
|---|---|---|
| SigMax 1Ab-PBS | 100 mL | |
| SigMax 2Ab-PBS | 100 mL | |
| HRPQuench-PBS | 100 mL |
Manufacturing & compliance
SigMax HRP-PBS Kit is manufactured under ISO 13485-certified and CE-approved facilities (suppliers of DiagnoCine Precision), with final packaging, quality assurance, and testing completed at the DiagnoCine R&D and Quality Testing Center.
ISO 13485:2016 QMS
Manufactured within an ISO 13485-certified quality management system.
Ultrapure Type 1 Water
Formulated with Ultrapure Type 1 water (18.2 MΩ·cm).
Controlled Fill Environment
Sequentially filtered (0.1 µm once, 0.04 µm once) prior to fill under controlled manufacturing conditions.
Custom Assembly Precision
Customization requests and kit assembly performed at DiagnoCine Precision, Totowa, New Jersey, USA.
Manufacturing Alignment ISO 13485
Facility manufacturing practices are aligned with ISO 13485-certified quality management principles.
CE-Approved Facility CE
Manufactured under CE-approved facility standards for supplied reagent kits.
pH Control USP <791>
Formulated and lot-checked for a defined pH 7.4 to minimize lot-to-lot variation.
Documentation / CoA
A Certificate of Analysis is available upon request — contact support@diagnocine.com.
How DCP-HRPPBSKit compares
A side-by-side view of SigMax HRP-PBS Kit against conventional single-pass filtered PBS reagents.
| Parameter | DCP-HRPPBSKit (FluxMPS™) | Conventional PBS (0.22 µm filtered) | Standard Alternative (0.22 µm filtered) |
|---|---|---|---|
| Defined pH & Lot-to-Lot Consistency | check_circle | cancel | cancel |
| Final Filtration Pore Size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of Filtration Stages | 2 | 1 | 1 |
| Water Quality | Ultrapure Type 1 (18.2 MΩ·cm) | cancel | cancel |
| Manufacturing QMS | ISO 13485:2016 | cancel | cancel |
| Unified Ab Dilution + HRP Quench Kit | check_circle | cancel | cancel |
| Automated Immunoassay Platform Compatibility | check_circle | cancel | cancel |
| Custom Formulation Available | check_circle | cancel | cancel |
Frequently asked questions
Common questions about SigMax HRP-PBS Kit.
Supporting literature
Curated literature relevant to HRP-based immunoassays, PBS buffer chemistry, and organ-on-a-chip applications.
- Josephy PD, Eling T, Mason RP. The horseradish peroxidase-catalyzed oxidation of 3,5,3',5'-tetramethylbenzidine. J Biol Chem. 1982. doi:10.1016/S0021-9258(19)45180-4
- Lequin RM. Enzyme immunoassay (EIA)/enzyme-linked immunosorbent assay (ELISA). Clin Chem. 2005. doi:10.1373/clinchem.2005.051532
- Mahmood T, Yang PC. Western blot: technique, theory, and trouble shooting. N Am J Med Sci. 2012. doi:10.4103/1947-2714.100998
- Ramos-Vara JA. Technical aspects of immunohistochemistry. Vet Pathol. 2005. doi:10.1354/vp.42-4-405
- Tighe PJ, Ryder RR, Todd I, Fairclough LC. ELISA in the multiplex era: potentials and pitfalls. Proteomics Clin Appl. 2015. doi:10.1002/prca.201400130
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014. doi:10.1038/nbt.2989
- Zeeberg K, et al. Assessing buffer conditions and pH effects on enzyme-linked detection assays. Anal Biochem. 2016. doi:10.1016/j.ab.2016.03.001
- Malmstadt N, et al. Automated microfluidic platforms for bioassay development. Anal Chem. 2003. doi:10.1021/ac034091s
- Rusling JF. Multiplexed electrochemical protein detection and translation to point-of-care use. Anal Chem. 2013. doi:10.1021/ac401075a
