SigMax ALP-TBS kit
FluxMPS™ SigMax ALP-TBS Kit
An MPS-grade, ultrapure TBS immunoassay buffer kit engineered for alkaline phosphatase (ALP)-based detection workflows in phosphate-free, TBS-buffered systems. Dual-stage 0.1 µm and 0.04 µm membrane filtration and Ultrapure Type 1 water deliver microchannel-safe cleanliness for ELISA, Western blot, and in situ hybridization protocols demanding low background and high signal fidelity.
- Filtered 0.1 µm membrane once and 0.04 µm membrane once (Dual-stage filtration architecture)
- QC-verified pH 7.4; formulated for stable performance across pH 7.0–8.5 TBS-buffered workflows
- 3-part kit: SigMax 1Ab-TBS, SigMax 2Ab-TBS, and ALPQuench-TBS, 100 mL each (3 x 100 mL bottles)
- Manufactured with Ultrapure Type 1 water (18.2 MΩ·cm)
- ISO 13485-certified, CE-approved manufacturing; final QA, testing, and assembly at DiagnoCine Precision, Totowa, New Jersey, USA
- Non-Sterile, Clear, Colorless Liquid; stored at 4°C with 1-year shelf life
- Custom concentrations, pH, and additive/supplement formulations available on request
- pH7.4
- Buffering RangepH 7.0–8.5 (TBS)
- Kit Size3 x 100 mL bottles
- AppearanceClear, colorless liquid
- SterilityNon-Sterile
- Filtration0.1 µm once + 0.04 µm once (Dual-stage)
- Water QualityUltrapure Type 1 (18.2 MΩ·cm)
- Storage4°C
- Shelf Life1 year
- Manufacturing Std.ISO 13485:2016 / CE-approved
Engineered where standard TBS reagents fall short
Conventional 0.22 µm-filtered buffers can carry subvisible particulates and residual bioburden into antibody dilution, blocking, and quenching steps, contributing to elevated background and inconsistent signal in ALP- and HRP-based immunoassays. SigMax ALP-TBS Kit is built to reduce these failure modes at every stage of the workflow.
Microchannel-safe purity
Dual-stage 0.1 µm and 0.04 µm membrane filtration reduces particulate carryover relative to a single 0.22 µm pass.
Precise, stable pH
QC-verified pH 7.4, formulated for stable performance across the pH 7.0–8.5 TBS-buffered workflow range.
Ultrapure-grade water
Formulated with Ultrapure Type 1 water (18.2 MΩ·cm) as the aqueous base for all three kit components.
Low background for imaging & assays
Proprietary antibody diluent and quenching blends are designed to maximize binding strength and minimize non-specific background.
Defined, traceable composition
Three named, lot-released components — SigMax 1Ab-TBS, SigMax 2Ab-TBS, and ALPQuench-TBS — each supplied at 100 mL.
Customization on demand
Alternate concentrations, additives, pH, and modifications available on request.
Dual-stage filtration system
Each of the three SigMax ALP-TBS Kit components is manufactured with a dual-stage membrane filtration process — a 0.1 µm pass followed by a 0.04 µm pass — supporting reduced particulate content ahead of use in sensitive immunoassay workflows.
-
1
0.1 µm Pre-filtration I
Removes larger particulates and aggregates, extending the working life of the downstream 0.04 µm filter.
-
2
0.04 µm Pre-filtration II
A finer second pass retains smaller particulates, including material near the size range of mycoplasma organisms.
Performance vs. conventional buffer
Sequential 0.1 µm and 0.04 µm membrane filtration removes finer particulates than a single 0.22 µm pass, supporting cleaner antibody diluent and quenching reagent delivery across ELISA, Western blot, and in situ hybridization workflows.
© Diagnocine® — DCP-ALPTBSKit
Where SigMax ALP-TBS Kit fits your workflow
SigMax ALP-TBS Kit was developed to support ALP-based immunoassays in TBS-buffered systems, with a proprietary diluent-and-quench chemistry that carries across ELISA, Western blotting, and in situ hybridization formats.
Automated Bioreactors & Robotics
An optional 0.01 µm (10 nm) ultra-filtered variant of the underlying TBS buffer components is available for automated bioreactor and liquid-handling robotics platforms where valve and sensor protection is a priority.
- Total Particulate Exclusion — supports automated fluidic paths sensitive to residual particulate
- Valve & Sensor Protection — reduces particulate load on microvalves and inline sensors
- Extended Perfusion Stability — consistent buffer quality across long automated runs
Inquiry Required: contact support@diagnocine.com to request the 0.01 µm ultra-filtered grade for automated bioreactor or robotics use.
Micro Physiological System (MPS) & Chip
TBS-based buffer components compatible with on-chip immunodetection and wash steps in microfluidic culture platforms.
Wash, Dilution & Reconstitution
SigMax 1Ab-TBS and SigMax 2Ab-TBS serve as TBS-based antibody diluents supporting wash and dilution steps in immunoassay preparation.
iPSC-Derived Model Handling
TBS-buffered reagents compatible with immunodetection workflows used in iPSC-derived model characterization.
Endothelial & Primary Cell Perfusion
Ultrapure TBS-based formulation suitable for immunodetection steps in endothelial and primary cell perfusion studies.
ELISA, Blotting & Blocking
Purpose-built for detection and quantification of autoantibodies in serum/plasma, viral antigen analysis, and multiplexed sepsis biomarker panel quantification in HRP- and ALP-based TBS immunoassays.
Microscopy & Optical Sensing
Low-background TBS formulation compatible with immunofluorescence and optical detection workflows on and off chip.
Measured and declared parameters
Specifications below reflect values stated for SigMax ALP-TBS Kit (DCP-ALPTBSKit) at release.
| Parameter | Specification |
|---|---|
| Formulation / Composition | 3-part kit: SigMax 1Ab-TBS, SigMax 2Ab-TBS, ALPQuench-TBS (100 mL each) |
| Appearance | Clear, colorless liquid |
| pH (USP <791>) | 7.4 |
| Buffering Range | pH 7.0–8.5 (TBS-buffered workflow) |
| Kit Size | 3 x 100 mL bottles |
| Parameter | Specification |
|---|---|
| Sterility | Non-Sterile |
| Filtration System | 0.1 µm membrane once + 0.04 µm membrane once (Dual-stage) USP <788> |
| Water Quality | Ultrapure Type 1 water (18.2 MΩ·cm) USP <85> |
| Manufacturing Standard | ISO 13485:2016 certified; CE-approved facility ISO 13485 |
| Parameter | Specification |
|---|---|
| Storage Temperature | 4°C |
| Shelf Life | 1 year |
| Parameter | Specification |
|---|---|
| Raw Material Grade | Ultrapure formulation components |
| Manufacturing QMS | ISO 13485:2016 certified (Suppliers of DiagnoCine Precision) ISO 13485 |
| Regulatory Alignment | CE-approved facility |
| Production Method | Final packaging, QA, and testing at the DiagnoCine R&D and Quality Testing Center; customization and assembly at DiagnoCine Precision, Totowa, New Jersey, USA |
| Intended Use | Research Use Only (RUO) |
Full composition
SigMax ALP-TBS Kit is supplied as three individually released TBS-based components.
| Component | CAS Number | Concentration |
|---|---|---|
| KIT COMPONENTS | ||
| SigMax 1Ab-TBS | — | 100 mL (1 x bottle) |
| SigMax 2Ab-TBS | — | 100 mL (1 x bottle) |
| ALPQuench-TBS | — | 100 mL (1 x bottle) |
Manufacturing & compliance
SigMax ALP-TBS Kit is manufactured under ISO 13485-certified, CE-approved facilities, with final packaging, quality assurance, and testing completed at the DiagnoCine R&D and Quality Testing Center.
ISO 13485:2016 & CE-Approved Manufacturing
Produced by suppliers of DiagnoCine Precision under an ISO 13485-certified, CE-approved quality system.
Ultrapure Type 1 Water
Formulated using Ultrapure Type 1 water (18.2 MΩ·cm) as the base for all three kit components.
Dual-Stage Membrane Filtration
Sequential 0.1 µm and 0.04 µm membrane filtration applied once each during manufacture.
DiagnoCine R&D & Quality Testing Center Release
Final packaging, QA, and testing performed in-house; customization and assembly completed at DiagnoCine Precision, Totowa, New Jersey, USA.
Sterility Status
Declared Non-Sterile; verified at release by the DiagnoCine Quality Testing Center.
Appearance QC
Clear, colorless liquid, confirmed at release.
pH Verification
QC-verified pH 7.4; formulated for stability across pH 7.0–8.5 TBS-buffered workflows.
Documentation / CoA
Certificate of Analysis available on request — contact support@diagnocine.com.
How DCP-ALPTBSKit compares
A general comparison against conventional single-pass filtered TBS reagents.
| Parameter | DCP-ALPTBSKit (FluxMPS™) | Conventional version (0.22 µm filtered buffer) | Standard alternative (0.22 µm filtered buffer) |
|---|---|---|---|
| Final filtration pore size | 0.1 µm + 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 2 | 1 | 1 |
| Water quality | check_circle Type 1 (18.2 MΩ·cm) | cancel | cancel |
| Manufacturing QMS | check_circle ISO 13485:2016 / CE | cancel | cancel |
| Microfluidic channel compatibility | check_circle | cancel | cancel |
| pH stability across TBS range (7.0–8.5) | check_circle | cancel | cancel |
| Custom formulation available | check_circle | cancel | cancel |
Frequently asked questions
Common questions about SigMax ALP-TBS Kit (DCP-ALPTBSKit).
Supporting literature
Curated literature relevant to TBS-buffered immunoassay workflows and organ-on-a-chip applications.
- Ingber, D.E. Human organs-on-chips for disease modeling, drug development, and personalized medicine. Nat Rev Genet. doi:10.1038/s41576-022-00466-9
- Bjornsson, C.S. et al. Perfusion buffer composition and its effect on microfluidic channel biofouling. Lab Chip. doi:10.1039/C9LC00889F
- Crowther, J.R. The ELISA Guidebook. Methods Mol Biol. doi:10.1385/1592590497
- Mahmood, T. & Yang, P.C. Western blot: technique, theory, and trouble shooting. N Am J Med Sci. doi:10.4103/1947-2714.100998
- Good, N.E. et al. Hydrogen ion buffers for biological research. Biochemistry. doi:10.1021/bi00866a011
- Solati, Z. et al. Autoantibody detection assays in rheumatologic disease research. Clin Chim Acta. doi:10.1016/j.cca.2020.02.014
- Rittirsch, D. et al. Immunodetection strategies for sepsis biomarker panels in translational and critical care research. Shock. doi:10.1097/SHK.0b013e3181e14f4a
- Bhatia, S.N. & Ingber, D.E. Microfluidic organs-on-chips. Nat Biotechnol. doi:10.1038/nbt.2989
