SigMax 2Ab-PBS
FluxMPS™ SigMax 2Ab-PBS
SigMax 2Ab-PBS is an MPS-grade, PBS-based secondary antibody enhancement buffer formulated at pH 7.4 with a defined blend of PEG 6000, Triton X-100, Tween-20, and recombinant Protein G fragments. Ultrapure and dual-stage filtered through 0.1 µm and 0.04 µm membranes, it is engineered to amplify secondary-to-primary antibody binding while suppressing non-specific background across ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, and phospho-specific Western blotting workflows.
- Dual-stage 0.1 µm + 0.04 µm membrane filtration for an ultrapure, particulate-controlled buffer
- PBS-based formulation enriched with PEG 6000, Glycine, Triton X-100, Tween-20, and recombinant Protein G fragments to enhance secondary antibody binding
- Clear, colorless liquid at pH 7.4 for consistent immunoassay performance
- Manufactured under ISO 13485-certified, CE-approved facilities
- Compatible with ELISA, IHC, ICC, flow cytometry, and phospho-specific Western blotting workflows
- Non-sterile, 100 mL format, stored at 4°C with a 1-year shelf life
- Customizable concentration, pH, and additive composition available on request
- pH7.4
- FormulationPBS-based with PEG 6000, Glycine, Triton X-100, Tween-20, Protein G fragments
- AppearanceClear, colorless liquid
- Filtration0.1 µm + 0.04 µm (dual-stage)
- SterilityNon-sterile
- Water QualityUltrapure Type 1 water
- Storage4°C
- Shelf Life1 year
- Size100 mL
- CustomizationAvailable on request
Engineered where standard antibody buffers fail
Conventional 0.22 µm-filtered secondary antibody buffers can carry subvisible particulates, inconsistent pH, and background-raising contaminants that erode signal-to-noise in multi-step immunodetection. SigMax 2Ab-PBS is built to remove those failure modes at the source.
Microchannel-safe purity
Dual-stage 0.1 µm and 0.04 µm membrane filtration reduces particulate carryover in multi-step immunodetection protocols.
Precise, stable pH
A PBS core buffered to pH 7.4 preserves antibody conformation and supports reproducible binding kinetics.
Ultrapure-grade water
Formulated with Ultrapure Type 1 water (18.2 MΩ·cm) to minimize background contribution to sensitive assays.
Low background for imaging & assays
The PEG 6000, Glycine, and Triton X-100/Tween-20 blend reduces non-specific binding, improving signal-to-noise across ELISA, IHC, ICC, and flow cytometry.
Defined, traceable composition
Each component — from recombinant Protein G fragments to trehalose and β-cyclodextrin stabilizers — is documented in the full composition table below.
Customization on demand
Alternate concentrations, additional chemicals, compounds, proteins, supplements, or pH targets are available on inquiry.
Dual-stage filtration system
SigMax 2Ab-PBS is Ultrapure and passes sequentially through a 0.1 µm membrane and a 0.04 µm membrane, reducing particulate load beyond what a single conventional filtration pass achieves.
-
1
0.1 µm Pre-filtration I
Removes large particulates and aggregates from the PBS-based formulation prior to final polishing.
-
2
0.04 µm Final Filtration — Polish
Retains fine particulates for an Ultrapure, clear, colorless liquid ready for sensitive multi-step immunodetection.
Performance vs. conventional buffer
Sequential 0.1 µm and 0.04 µm membrane filtration removes finer particulates than a single 0.22 µm pass typical of conventional secondary antibody buffers, supporting cleaner multi-step immunodetection protocols.
© Diagnocine® — DCP-SM2AbPBS1X
Where SigMax 2Ab-PBS is used
SigMax 2Ab-PBS establishes an optimal microenvironment for secondary-to-primary antibody interactions, delivering signal amplification while minimizing background across common immunodetection platforms.
Automated Bioreactors & Robotics
An optional 0.01 µm (10 nm) ultra-filtered variant of SigMax 2Ab-PBS is available for automated bioreactor and robotic liquid-handling platforms where valve and sensor protection is critical.
- Total Particulate Exclusion for sensitive fluidic pathways
- Valve & Sensor Protection in automated dispensing systems
- Extended Perfusion Stability for continuous-flow protocols
Inquiry Required: contact support@diagnocine.com to request the 0.01 µm ultra-filtered grade.
ELISA Signal Enhancement
Optimizes secondary antibody binding affinity to improve detection sensitivity and reduce background in ELISA protocols.
Western Blotting — Phospho-Proteins
Formulated for phospho-specific antibody applications, improving clarity in Western blotting of phospho-proteins.
Flow Cytometry Labeling
Reduces non-specific binding during antibody labeling steps, improving signal-to-noise in flow cytometry panels.
Immunohistochemistry & Immunocytochemistry
Supports high-clarity multi-step detection in IHC and ICC protocols with reduced non-specific binding.
Micro Physiological System (MPS) & Chip
Ultrapure, dual-filtered formulation suited for on-chip immunodetection steps within MPS and lab-on-a-chip workflows.
Secondary Antibody Conservation
Allows for lower primary and secondary antibody concentrations, conserving valuable reagents while maintaining sensitivity.
Specification data
All values below are as declared for SigMax 2Ab-PBS, Cat. No. DCP-SM2AbPBS1X.
| Parameter | Specification |
|---|---|
| Formulation / Composition | PBS-based buffer with PEG 6000, Glycine, Triton X-100, Tween-20, recombinant Protein G fragments and stabilizers — see full composition table below |
| Appearance | Clear, Colorless Liquid |
| pH | 7.4 |
| Parameter | Specification |
|---|---|
| Sterility | Non-sterile |
| Water Quality USP <85> | Ultrapure Type 1 water (18.2 MΩ·cm) |
| Manufacturing Standard ISO | ISO 13485-certified, CE-approved facilities |
| Parameter | Specification |
|---|---|
| Storage Temperature | 4°C |
| Shelf Life | 1 year |
| Parameter | Specification |
|---|---|
| Manufacturing QMS ISO 13485 | ISO 13485-certified, CE-approved (DiagnoCine Precision) |
| Production & Assembly Site | DiagnoCine Precision, Totowa, New Jersey, USA |
| Final QA & Packaging | DiagnoCine R&D and Quality Testing Center |
| Intended Use | Research Use Only (RUO) |
Full composition
Every component of SigMax 2Ab-PBS and its declared concentration is listed below.
| Component | CAS Number | Concentration |
|---|---|---|
| Sodium Chloride (NaCl) | 7647-14-5 | 137 mM |
| Potassium Chloride (KCl) | 7447-40-7 | 2.7 mM |
| Sodium Phosphate dibasic (Na2HPO4) | 7558-79-4 | 10 mM |
| Potassium Phosphate monobasic (KH2PO4) | 7778-77-0 | 1.8 mM |
| Polyethylene Glycol 6000 (PEG 6000) | 25322-68-3 | 5.0% (w/v) |
| Glycine | 56-40-6 | 0.75% (w/v) |
| Triton X-100 | 9002-93-1 | 0.05% (v/v) |
| Tween-20 | 9005-64-5 | 0.1% (v/v) |
| Recombinant Protein G fragments | 0.00005% (w/v) | |
| Hydrogen peroxide (H2O2) | 7722-84-1 | 0.03% (v/v) |
| Trehalose | 99-20-7 | 1.0% (w/v) |
| β-Cyclodextrin | 7585-39-9 | 0.1% (w/v) |
| ProClin 300 | 0.05% (v/v) |
Manufacturing & compliance
SigMax 2Ab-PBS is produced, packaged, and tested under a documented quality management system.
ISO 13485:2016 QMS
Manufactured under ISO 13485-certified and CE-approved supplier facilities.
Ultrapure Type 1 Water
Formulated with Ultrapure water to minimize background in sensitive immunoassays.
Dual-Stage Filtration
Sequential 0.1 µm and 0.04 µm membrane filtration for a clear, colorless, Non-sterile buffer.
Micro-Batch Precision
Final packaging, quality assurance, and testing performed at the DiagnoCine R&D and Quality Testing Center; customization assembled at DiagnoCine Precision, Totowa, New Jersey, USA.
Non-Sterile Formulation
SigMax 2Ab-PBS is manufactured and released as a Non-sterile reagent for research immunoassay use.
Composition Traceability
Each of the 12 declared components is documented with its concentration in the full composition table above.
Storage & Shelf-life Assurance
Stored at 4°C, SigMax 2Ab-PBS carries a 1-year shelf life.
Documentation / CoA
A Certificate of Analysis is available for this lot.
How DCP-SM2AbPBS1X compares
A side-by-side view of SigMax 2Ab-PBS against conventional secondary antibody buffers and standard PBS-Tween wash buffer.
| Parameter | DCP-SM2AbPBS1X (FluxMPS™) | Conventional Secondary Antibody Buffer (0.22 µm filtered) | Standard PBS-Tween Wash Buffer |
|---|---|---|---|
| Antibody-binding enhancer blend (PEG 6000, Protein G fragments) | check_circle | cancel | cancel |
| Final filtration pore size | 0.04 µm | 0.22 µm | Not specified |
| Number of filtration stages | 2 | 1 | Not specified |
| Buffered to pH 7.4 | check_circle | check_circle | check_circle |
| Non-specific binding reduction blend | check_circle | cancel | cancel |
| Compatible with ELISA, IHC, ICC, flow cytometry, phospho-WB | check_circle | check_circle | cancel |
| Custom formulation on request | check_circle | cancel | cancel |
| Manufactured under ISO 13485-certified, CE-approved facility | check_circle | cancel | cancel |
Frequently asked questions
Common questions about SigMax 2Ab-PBS, Cat. No. DCP-SM2AbPBS1X.
Supporting literature
Curated literature relevant to secondary antibody enhancement buffers, PBS chemistry, and immunodetection.
- Kausaite-Minkstimiene A, et al. Comparative study of random and oriented antibody immobilization techniques for immunoassay development. doi:10.1021/ac902758c
- Kingsmore SF. Multiplexed protein measurement: technologies and applications of protein and antibody arrays. Nat Rev Drug Discov. doi:10.1038/nrd2149
- Sikorski Z, et al. Polyethylene glycol enhancement of antigen-antibody interactions in immunoassays. doi:10.1016/0022-1759(85)90267-1
- Butler JE. Solid supports in enzyme-linked immunosorbent assay and other solid-phase immunoassays. Methods. doi:10.1006/meth.2000.1027
- Akerstrom B, Bjorck L. Protein G: a powerful tool for binding and detection of monoclonal and polyclonal antibodies. J Immunol. doi:10.4049/jimmunol.135.4.2589
- Mahmood T, Yang PC. Western blot: technique, theory, and trouble shooting. N Am J Med Sci. doi:10.4103/1947-2714.100998
- Ramos-Vara JA. Technical aspects of immunohistochemistry. Vet Pathol. doi:10.1354/vp.42-4-405
- Robinson JP, Roederer M. History of science. Flow cytometry strikes gold. Science. doi:10.1126/science.1237506
- Jiskoot W, et al. Application of trehalose and cyclodextrins as stabilizers in protein and antibody formulations. Pharm Res. doi:10.1023/A:1011916017040
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. doi:10.1038/nbt.2989
