SDS Gel Loading Buffer [5X]
Cat No.: DCP-SDSLB5X
Size: 5 x 1 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description:
The SDS Loading Buffer helps to denature proteins into individual polypeptides by disrupting their 3D structure using SDS and heat. Using SDS, it applies a negative charge to the denatured proteins proportional to their size. The addition of glycerol increases the density of the protein sample, which causes the sample to sink to the bottom of the gel wells during loading and prevents it from diffusing into the running buffer. The addition of bromophenol blue serves as a visible tracking dye to monitor the migration of proteins through the gel during electrophoresis and provides aid in the proper loading of the sample into the wells of the gel. β-mercaptoethanol is a powerful reducing agent that can break the disulfide bridges within proteins and between protein subunits, which disrupts tertiary and quaternary structures of proteins.
Advantages:
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Denature proteins and apply a negative charge to the proteins
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Denatures proteins by disrupting non-covalent bonds, unfolding secondary, tertiary, and quaternary structures
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Keep denatured proteins soluble and aid in sample loading and tracking
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increases the density of the sample, causing it to sink to the bottom of the well when loaded onto the gel, preventing sample diffusion and ensuring proper loading.
Composition:
| Ingredients |
Concentration |
| Tris-HCl |
250 mM |
| SDS |
10% SDS |
| Glycerol |
30% Glycerol |
| TCEP |
50 mM |
| Bromophenol blue |
0.02% |
Quality Control:
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Appearance: Blue Liquid.
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pH: 6.8
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Sterility: Filtered 0.1 micron Twice and 0.04 micron once in a sterile environment.
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DNase activity: None was detected after incubation of plasmid DNA, and this product was used for 18 hours at room temperature.
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RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
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Storage: -20 ℃
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Shelf life: 1 year
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
