Ribonuclease R (RNase R)

Ribonuclease R (RNase R) 

Cat. No.: SB-G3462-500U
Size: 500U


Description

Ribonuclease R, referred to as RNase R, is a 3'-5' exoribonuclease derived from Escherichia coli, which is recombinantly expressed in Escherichia coli, and gradually cuts RNA into dinucleotides and trinucleotides from the 3'-5' direction . It can degrade almost all linear RNA molecules and Y-structure RNA, but it is not easy to degrade circular RNA (circRNA), lariat structure (lariat RNA) and double-stranded RNA molecules with 3' protruding ends of less than 7 nucleotides. Based on its characteristics, it is mainly used for gene expression and variable splicing research, enriching circular RNA or lariat RNA.

• Source : derived from Escherichia coli, recombinantly expressed by Escherichia coli.
• Definition of enzyme activity : under standard reaction conditions at 37°C, the amount of enzyme required to convert 1 μg poly(A) into acid-soluble nucleotides within 10 minutes is defined as one enzyme activity unit.
• Purity and concentration : SDS-PAGE detection purity ≥ 95%; 20 U/μL.
• Enzyme storage buffer : 50 mM Tris-HCl, 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100, 50% Glycerol, pH7.5.
• 10 × Reaction Buffer :  200 mM Tris-HCl, 1 M KCl, 1 mM MgCl₂ , pH 8.0.

Storage and Handling Conditions

1. All enzymes should be stored in an ice box and stored at -20°C immediately after use.
2. For your safety and health, please wear a lab coat and disposable gloves for operation.