Product Overview

ReverTra Ace® qPCR RT Kit
Price per reaction $1.13

Description
The ReverTra Ace® qPCR RT Kit efficiently synthesizes high quality cDNAs from RNA templates for real-time PCR. This kit contains the reverse transcriptase "ReverTra Ace®" and a RT buffer optimized for the synthesis of short-chain cDNAs suitable for real-time PCR. The protocol is simple, and the reaction can be completed in just 15 minutes. ReverTra Ace® is a mutant-type M-MLV reverse transcriptase that is one of the best of its class in the market today.
The protocol is simple, and the reaction can be completed in 15 min.
ReverTra Ace^® is a mutant-type M-MLV reverse transcriptase shows excellent efficiency.
ReverTra Ace^® qPCR RT Master Mix (Code No. TYB-FSQ-201) is a premix version of ReverTra Ace^® qPCR RT Kit. The master mix reagent (5x) contains the highly efficient reverse transcriptase "ReverTra Ace^®" , primers and buffer optimized for highly efficient synthesis of short-chain cDNAs suitable for real-time PCR.

Features

• The optimized RT buffer and primer mix enable highly efficient reverse transcription.
• The reaction can be completed in 15 minutes. The protocol does not contain an additional RNase H treatment step to remove residual RNA after reverse transcription (patent pending).
• Suitable for the detection of low-expressing mRNAs. Since the RT buffer is optimized for real-time PCR, the addition of 20% (v/v) of the synthesized cDNA solution to the PCR solution does not inhibit the PCR reaction.

Specifications

• Robust M-MLV RT enzyme
• 10µl reaction volume per sample
• 5x RT Buffer

Applications
cDNA synthesis for real-time PCR
 
Components

• 5x RT Buffer 400 µl
• Enzyme Mix 100 µl
• Primer Mix 100 µl
• Nuclease-free water 1000 µl × 2

Application data
Example 1. Comparison of cDNA yields using various reverse transcription kits
cDNA was synthesized from human cell line (HeLa cell) total RNA with the ReverTra Ace® qPCR RT kit. Subsequently, quantification of β-actin and polymerase ε mRNA were performed by real-time PCR with 100 ng cDNA, in conjunction with the SYBR® Green Realtime PCR Master Mix [Code No. TYB-QPK-201].

Example 2. Detection of a rare expressing gene
Several 1-µl cDNA aliquots were prepared from 100 ng human cell line total RNA in a volume of 20 µl using various reverse transcription kits. Subsequently, a low expressing gene (TNF-α) was detected with real-time PCR kit (SYBR® Green Realtime PCR Master Mix [Code No. TYB-QPK-201].
The Ct values and success rates of the ReverTra Ace qPCR RT kit were greater than those from the other commercial kits