SigMax 1Ab-TBS: add to ELISA protocol when increased signal intensity is required
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Rat Enolase, Neuron Specific (NSE)
Cat.No.: JNB-JL13282
Size: 96T
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.21ng/mL
Detection Range: 0.62-40ng/mL
Alternative Names: ENO2; Enolase 2; Gamma Enolase; 2-phospho-D-glycerate hydro-lyase; Neural enolase;2 phospho D glycerate hydrolyase;2-phospho-D-glycerate hydro-lyase;Eno 2;ENOG;ENOG_HUMAN;Enolase 2 (gamma, neuronal);Enolase 2 gamma neuronal;Enolase2;Epididymis secretory protein Li 279;Gamma enolase;Gamma-enolase;HEL S 279;Neural enolase;Neuron specific enolase;Neuron specific gamma enolase;Neuron-specific enolase;neuronal enriched enolase;Neurone specific enolase;NSE
Research Areas: Cancer
Assay Time: 2h45m
Abbreviation: NSE
Test Principle: This assay employs the sandwich enzyme immunoassay technique. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NSE. Add Sample, Standard, Biotin-antibody, and Streptavidin-HRP by turn, with incubation and washing. TMB Substrate is added for color development, and TMB will appear blue in color catalyzed by HRP and to a final yellow color in reaction to acid. The intensity of the color is positive to the Rat NSE in the sample. The optical density (OD) is measured spectrophotometrically at a wavelength of 450nm. You can calculate the concentration of Rat NSE in the samples by comparing the OD of the samples to the standard curve.
Specificity: This kit recognizes Rat NSE in samples. No significant cross-reactivity or interference between Rat NSE and analogues was observed.