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Rat Vasopressin V1B Receptor, AVPR1B ELISA Kit
Product#: BTL-E1478Ra
$686.40
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Rat Vasopressin V1B Receptor, AVPR1B ELISA Kit
Cat. No#: BTL-E1478Ra
*All types, size 48T, of ELISA kits are available, including special MOQ value pricing. For more details, please contact us at support@diagnocine.com
Product Summary
Size: ,96T
Sensitivity: 0.02ng/ml
Detection range: 0.05-30ng/ml
Sample type: Serum, plasma, cell culture supernates
Reactive with: Rat
Product overview
Full product name
Vasopressin V1b receptor
SKU#.: ? BTL- |
E1478Ra |
Assay type |
Sandwich |
Size |
0.02ng/ml |
Detection range |
0.05-30ng/ml |
Sample type |
Serum, plasma, cell culture supernates |
Species |
Rat |
Storage |
2-8ºC |
Assay time |
1h 30m |
Background |
Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate a phosphatidyl-inositol-calcium second messenger system. |
UniProt accession |
47,086 |
GeneID |
29462 |
Synonyms |
Antidiuretic hormone receptor 1b;AVPR V1b;AVPR V3;AVPR1B;V1bR;Vasopressin V1b receptor;Vasopressin V3 receptor |
Gene names |
Avpr1b |
Research area |
Others |
Target protein |
AVPR1B |
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E1478Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 16ng/ml | 2.242 | 2.31 | 2.267 |
| 2.378 | |||
| 8ng/ml | 1.192 | 1.211 | 1.167 |
| 1.23 | |||
| 4ng/ml | 0.739 | 0.687 | 0.644 |
| 0.636 | |||
| 2ng/ml | 0.489 | 0.476 | 0.432 |
| 0.463 | |||
| 1ng/ml | 0.24 | 0.237 | 0.193 |
| 0.234 | |||
| 0ng/ml | 0.061 | 0.044 | 0 |
| 0.026 |
Precision
We measured random samples of E1478Ra within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 7.56 | 0.41 | 5.46 |
| Intra-Assay | 2 | 18 | 8.2 | 0.29 | 3.51 |
| Intra-Assay | 3 | 18 | 3.17 | 0.19 | 6.07 |