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Rat Guanylate Cyclase Soluble Subunit Alpha-2, GUCY1A2 ELISA Kit
Product#: BTL-E2002Ra
$686.40
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Rat Guanylate Cyclase Soluble Subunit Alpha-2, GUCY1A2 ELISA Kit
Cat. No#: BTL-E2002Ra
*All types, size 48T, of ELISA kits are available, including special MOQ value pricing. For more details, please contact us at support@diagnocine.com
Product Summary
Size: ,96T
Sensitivity: 7.34ng/L
Detection range: 15-3000ng/L
Sample type: Serum, plasma, cell culture supernates
Reactive with: Rat
Product overview
Full product name
Guanylate cyclase soluble subunit alpha-2
SKU#.: ? BTL- |
E2002Ra |
Assay type |
Sandwich |
Size |
7.34ng/L |
Detection range |
15-3000ng/L |
Sample type |
Serum, plasma, cell culture supernates |
Species |
Rat |
Storage |
2-8ºC |
Assay time |
1h 30m |
Background |
Has guanylyl cyclase on binding to the beta-1 subunit. |
UniProt accession |
81,787 |
GeneID |
66012 |
Synonyms |
GCS-alpha-2;Guanylate cyclase soluble subunit alpha-2;GUCY1A2 |
Gene names |
Gucy1a2 |
Research area |
Signal Transduction |
Target protein |
GUCY1A2 |
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2002Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1600ng/L | 2.098 | 2.243 | 2.208 |
| 2.389 | |||
| 800ng/L | 1.247 | 1.249 | 1.213 |
| 1.251 | |||
| 400ng/L | 0.723 | 0.725 | 0.689 |
| 0.728 | |||
| 200ng/L | 0.442 | 0.427 | 0.391 |
| 0.411 | |||
| 100ng/L | 0.146 | 0.155 | 0.119 |
| 0.163 | |||
| 0ng/L | 0.034 | 0.036 | 0 |
| 0.038 |
Precision
We measured random samples of E2002Ra within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 736.85 | 42.01 | 5.7 |
| Intra-Assay | 2 | 18 | 920.77 | 34.63 | 3.76 |
| Intra-Assay | 3 | 18 | 310.64 | 17.01 | 5.47 |