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Rat Glutaminase Kidney Isoform, Mitochondria, GLS ELISA Kit
Product#: BTL-E1984Ra
$686.40
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Rat Glutaminase Kidney Isoform, Mitochondria, GLS ELISA Kit
Cat. No#: BTL-E1984Ra
*All types, size 48T, of ELISA kits are available, including special MOQ value pricing. For more details, please contact us at support@diagnocine.com
Product Summary
Size: ,96T
Sensitivity: 14.34ng/L
Detection range: 15-3000ng/L
Sample type: Serum, plasma, cell culture supernates
Reactive with: Rat
Product overview
Full product name
Glutaminase Kidney Isoform, Mitochondria
SKU#.: ? BTL- |
E1984Ra |
Assay type |
Sandwich |
Size |
14.34ng/L |
Detection range |
15-3000ng/L |
Sample type |
Serum, plasma, cell culture supernates |
Species |
Rat |
Storage |
2-8ºC |
Assay time |
1h 30m |
Background |
Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate, the main excitatory neurotransmitter in the brain. |
UniProt accession |
74,024 |
GeneID |
24398 |
Synonyms |
GLS;Glutaminase kidney isoform, mitochondrial;Glutaminase kidney isoform, mitochondrial 65 kDa chain;Glutaminase kidney isoform, mitochondrial 68 kDa chain;K-glutaminase;L-glutamine amidohydrolase |
Gene names |
GLS |
Research area |
Others |
Target protein |
GLS |
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E1984Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1600ng/L | 2.297 | 2.339 | 2.288 |
| 2.382 | |||
| 800ng/L | 1.45 | 1.392 | 1.341 |
| 1.334 | |||
| 400ng/L | 0.709 | 0.74 | 0.688 |
| 0.77 | |||
| 200ng/L | 0.423 | 0.425 | 0.374 |
| 0.427 | |||
| 100ng/L | 0.18 | 0.193 | 0.142 |
| 0.206 | |||
| 0ng/L | 0.049 | 0.051 | 0 |
| 0.054 |
Precision
We measured random samples of E1984Ra within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 403.8 | 22.32 | 5.5 |
| Intra-Assay | 2 | 18 | 925.1 | 36.38 | 3.9 |
| Intra-Assay | 3 | 18 | 584 | 29.61 | 5.1 |