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Rat Cytoplasmic Dynein 1 Heavy Chain 1, DYNC1H1 ELISA Kit
Product#: BTL-E1943Ra
$686.40
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Rat Cytoplasmic Dynein 1 Heavy Chain 1, DYNC1H1 ELISA Kit
Cat. No#: BTL-E1943Ra
*All types, size 48T, of ELISA kits are available, including special MOQ value pricing. For more details, please contact us at support@diagnocine.com
Product Summary
Size: ,96T
Sensitivity: 9.34ng/L
Detection range: 20-4500ng/L
Sample type: Serum, plasma, cell culture supernates
Reactive with: Rat
Product overview
Full product name
Cytoplasmic dynein 1 heavy chain 1
SKU#.: ? BTL- |
E1943Ra |
Assay type |
Sandwich |
Size |
9.34ng/L |
Detection range |
20-4500ng/L |
Sample type |
Serum, plasma, cell culture supernates |
Species |
Rat |
Storage |
2-8ºC |
Assay time |
1h 30m |
Background |
Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Plays a role in mitotic spindle assembly and metaphase plate congression. |
UniProt accession |
532,252 |
GeneID |
29489 |
Synonyms |
Cytoplasmic dynein 1 heavy chain 1;Cytoplasmic dynein heavy chain 1;DHC1a;Dnch1;Dnchc1;DYNC1H1;Dynein heavy chain, cytosolic;MAP 1C |
Gene names |
Dync1h1 |
Research area |
Others |
Target protein |
DYNC1H1 |
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E1943Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 2400ng/L | 2.037 | 2.149 | 2.082 |
| 2.262 | |||
| 1200ng/L | 1.491 | 1.416 | 1.349 |
| 1.341 | |||
| 600ng/L | 0.861 | 0.87 | 0.803 |
| 0.88 | |||
| 300ng/L | 0.458 | 0.448 | 0.381 |
| 0.438 | |||
| 150ng/L | 0.285 | 0.275 | 0.208 |
| 0.265 | |||
| 0ng/L | 0.071 | 0.067 | 0 |
| 0.063 |
Precision
We measured random samples of E1943Ra within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 1030.06 | 36.18 | 3.51 |
| Intra-Assay | 2 | 18 | 357.26 | 18.3 | 5.12 |
| Intra-Assay | 3 | 18 | 852.58 | 43.42 | 5.09 |