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Rat Apelin Receptor, APLNR ELISA Kit
Product#: BTL-E1027Ra
$686.40
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Rat Apelin Receptor, APLNR ELISA Kit
Cat. No#: BTL-E1027Ra
*All types, size 48T, of ELISA kits are available, including special MOQ value pricing. For more details, please contact us at support@diagnocine.com
Product Summary
Size: ,96T
Sensitivity: 0.09ng/ml
Detection range: 0.2- 60ng/ml
Sample type: Serum, plasma, cell culture supernates
Reactive with: Rat
Product overview
Full product name
Apelin Receptor
SKU#.: ? BTL- |
E1027Ra |
Assay type |
Sandwich |
Size |
0.09ng/ml |
Detection range |
Serum, plasma, cell culture supernates |
Species |
Rat |
Storage |
2-8ºC |
Assay time |
1h 30m |
Background |
Receptor for apelin receptor early endogenous ligand (APELA) and apelin (APLN) hormones coupled to G proteins that inhibit adenylate cyclase activity (PubMed: 11359874). Plays a key role in early development such as gastrulation, blood vessels formation and heart morphogenesis by acting as a receptor for APELA hormone. May promote angioblast migration toward the embryonic midline, i.e. the position of the future vessel formation, during vasculogenesis. Promotes sinus venosus (SV)-derived endothelial cells migration into the developing heart to promote coronary blood vessel development. Plays also a role in various processes in adults such as regulation of blood vessel formation, blood pressure, heart contractility and heart failure (By similarity). |
UniProt accession |
42,349 |
GeneID |
83518 |
Synonyms |
Angiotensin receptor-like 1;Apelin receptor;APLNR;B78;GPCR34;G-protein coupled receptor APJ |
Gene names |
APLNR |
Research area |
Others |
Target protein |
APLNR |
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E1027Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 40ng/ml | 2.242 | 2.159 | 2.125 |
| 2.075 | |||
| 20ng/ml | 1.716 | 1.6 | 1.566 |
| 1.485 | |||
| 10ng/ml | 0.968 | 0.968 | 0.934 |
| 0.968 | |||
| 5ng/ml | 0.691 | 0.678 | 0.644 |
| 0.665 | |||
| 2.5ng/ml | 0.409 | 0.41 | 0.376 |
| 0.411 | |||
| 0ng/ml | 0.037 | 0.034 | 0 |
| 0.031 |
Precision
We measured random samples of E1027Ra within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |