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Rat Activity-regulated Cytoskeleton-associated Protein, ARC ELISA Kit
Product#: BTL-E1842Ra
$686.40
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Rat Activity-regulated Cytoskeleton-associated Protein, ARC ELISA Kit
Cat. No#: BTL-E1842Ra
*All types, size 48T, of ELISA kits are available, including special MOQ value pricing. For more details, please contact us at support@diagnocine.com
Product Summary
Size: ,96T
Sensitivity: 0.48ng/L
Detection range: 1-400ng/L
Sample type: Serum, plasma, cell culture supernates
Reactive with: Rat
Product overview
Full product name
Activity-Regulated Cytoskeleton-Associated Protein
SKU#.: ? BTL- |
E1842Ra |
Assay type |
Sandwich |
Size |
0.48ng/L |
Detection range |
1-400ng/L |
Sample type |
Serum, plasma, cell culture supernates |
Species |
Rat |
Storage |
2-8ºC |
Assay time |
1h 30m |
Background |
Master regulator of synaptic plasticity that self-assembles into virion-like capsids that encapsulate RNAs and mediate intercellular RNA transfer in the nervous system (PubMed: 29328916). ARC protein is released from neurons in extracellular vesicles that mediate the transfer of ARC mRNA into new target cells, where ARC mRNA can undergo activity-dependent translation (PubMed: 29328916). ARC capsids are endocytosed and are able to transfer ARC mRNA into the cytoplasm of neurons (PubMed: 29328916). Acts as a key regulator of synaptic plasticity: required for protein synthesis-dependent forms of long-term potentiation (LTP) and depression (LTD) and for the formation of long-term memory (PubMed: 10818134, PubMed: 17088211, PubMed: 17088212, PubMed: 17088213). Regulates synaptic plasticity by promoting endocytosis of AMPA receptors (AMPARs) in response to synaptic activity: this endocytic pathway maintains levels of surface AMPARs in response to chronic changes in neuronal activity through synaptic scaling, thereby contributing to neuronal homeostasis (PubMed: 17088211, PubMed: 17088212). Acts as a postsynaptic mediator of activity-dependent synapse elimination in the developing cerebellum by mediating elimination of surplus climbing fiber synapses (PubMed: 23791196). Accumulates at weaker synapses, probably to prevent their undesired enhancement (PubMed: 22579289). This suggests that ARC-containing virion-like capsids may be required to eliminate synaptic material (PubMed: 29328916). Required to transduce experience into long-lasting changes in visual cortex plasticity and for long-term memory (PubMed: 10818134). Involved in postsynaptic trafficking and processing of amyloid-beta A4 (APP) via interaction with PSEN1 (By similarity). In addition to its role in synapses, also involved in the regulation of the immune system: specifically expressed in skin-migratory dendritic cells and regulates fast dendritic cell migration, thereby regulating T-cell activation (By similarity). |
UniProt accession |
45,353 |
GeneID |
54323 |
Synonyms |
Activity-regulated cytoskeleton-associated protein;Activity-regulated gene 3.1 protein;ARC;ARC/ARG3.1;Arg3.1 |
Gene names |
ARC |
Research area |
Neuroscience |
Target protein |
ARC |
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E1842Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 240ng/L | 2.146 | 2.232 | 2.185 |
| 2.319 | |||
| 120ng/L | 1.131 | 1.216 | 1.168 |
| 1.3 | |||
| 60ng/L | 0.832 | 0.774 | 0.726 |
| 0.715 | |||
| 30ng/L | 0.462 | 0.433 | 0.386 |
| 0.403 | |||
| 15ng/L | 0.199 | 0.187 | 0.139 |
| 0.174 | |||
| 0ng/L | 0.055 | 0.047 | 0 |
| 0.039 |
Precision
We measured random samples of E1842Ra within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 84.8 | 4.2 | 5 |
| Intra-Assay | 2 | 18 | 176.4 | 5.66 | 3.2 |
| Intra-Assay | 3 | 18 | 52.5 | 3.37 | 6.4 |