FluxMPS™ RPMI 1640 Medium w/o Sodium Pyruvate: 1X Liquid
FluxMPS™ RPMI 1640 Medium w/o Sodium Pyruvate: 1X Liquid
FluxMPS™ DCP-RPMI-P1X is a Microfluidics Suitable, ultra-filtered RPMI 1640 formulation engineered for T cells, B cells, NK cells, PBMCs, and hematopoietic cancer lines on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. Processed through a Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2), it reaches a 0.04 µm final cut-off — five times finer than the 0.22 µm membranes used for conventional sterile filtration. Formulation: [+] 2 g/L Glucose, [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red | [-] Sodium Pyruvate.
- High-glucose RPMI 1640 base (2000 mg/L / 2.0 g/L D-Glucose) with L-Glutamine (300 mg/L) and sodium bicarbonate buffering (2000 mg/L) for standard 5% CO₂ incubation
- Sodium pyruvate omitted by design, allowing researchers to add a defined pyruvate concentration for OXPHOS/glycolytic-flux studies in lymphocytes and hematopoietic cell lines
- Reduced glutathione (1.000 mg/L) included for redox and antioxidant support in redox-sensitive immune cell types
- Processed through a Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final cut-off
- Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), controlled per manufacturing batch
- Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
- Formulated with phenol red (5.300 mg/L) as a pH indicator — phenol-red-free variants available on request
- Ultrapure Type 1 water (18.2 MΩ·cm) used in formulation, with trace-metal and organic-carbon (TOC) control
- Media familyRPMI 1640
- Formulation[+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose (2 g/L) | [-] Sodium Pyruvate
- AppearanceRed-colored, clear solution
- pH (USP <791>)7.4
- Osmolality (USP <785>)280–320 mOsm/kg H2O
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2
- Storage2–8°C, protect from light
- Shelf Life12 months from date of manufacture, unopened
- ShippingCold pack
Available sizes: 500 mL, 1000 mL
Engineered where standard media fails
Conventional 0.22 µm–filtered RPMI passes mycoplasma-sized particles, subvisible particulates, and microaggregates that can accumulate in microfluidic channels and confound immune cell assays. FluxMPS™ is built to address these failure modes.
Microchannel-safe purity
0.04 µm final filtration; USP <788> particulate testing. Formulated for suspension immune cell OoC and flow cytometry workflows where particulate load matters.
Total metabolic control
Sodium pyruvate is omitted by design, letting you define the exact secondary carbon source and concentration for OXPHOS and glycolytic-flux studies, alongside 2.0 g/L D-glucose and 300 mg/L L-glutamine already in the base formulation.
Ultrapure-grade water
Type 1 water (18.2 MΩ·cm) is used in formulation, controlling trace-metal and organic-carbon (TOC) contamination during manufacture.
Low background for imaging
Sub-micron filtration reduces particulate background for flow cytometry, ELISA, and confocal imaging workflows. Note: this formulation contains phenol red and riboflavin; a phenol-red-free FluxMPS™ variant is available where optical/fluorescence background must be minimized.
Rich, stable nutrient profile
RPMI 1640 contains reduced glutathione and a balanced profile of vitamins and amino acids optimized for lymphocyte and hematopoietic cell culture, released per lot.
Customization on demand
pH, nutrient concentrations, HEPES, and component modifications available. Contact support@diagnocine.com.
Quadruple-stage filtration system
Four serial filtration passes — two dedicated prefilter + final-filter pairs — reaching a final 0.04 µm polish.
-
1
0.1 µm Prefiltration I
Removes large particulate, cell debris and protein aggregates; protects the first 0.04 µm cartridge.
-
2
0.04 µm Final filtration I
First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a 0.22 µm filter.
-
3
0.1 µm Prefiltration II
Second dedicated prefilter, protecting the second 0.04 µm cartridge.
-
4
0.04 µm Final filtration II — Polish
Ultimate polishing filter; aseptic fill & finish.
Performance vs. conventional media
A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration, with mycoplasma-retentive prefiltration applied at every production stage.
© Diagnocine® — DCP-RPMI-P1X
Immune cell OoC and hematopoietic applications
FluxMPS™ DCP-RPMI-P1X — RPMI 1640 w/o Sodium Pyruvate — delivers 0.04 µm filtered, Microfluidics Suitable purity for hematopoietic cell and immune cell OoC applications.
Automated Bioreactors & Robotics
An optional 0.01 µm (10 nm) MPS Grade variant (0.1 µm ×2 + 0.04 µm ×2 + 0.02 µm + 0.01 µm) is available on request for automated, high-throughput bioreactor and robotic liquid-handling systems where nanoparticulate exclusion is critical.
- Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates
- Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion systems
- Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture
Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.
T Cell & Lymphocyte Culture
RPMI 1640 is the standard base for primary T cells, B cells, NK cells, and monocytes. FluxMPS™ 0.04 µm filtration is intended to reduce particulate load in immune cell assays.
Leukemia & Lymphoma Lines
RPMI supports Jurkat, Raji, K562, HL-60, and other hematopoietic cancer lines where DMEM-based media would alter proliferation and signaling.
Immune Cell OoC
0.04 µm filtered RPMI for tumor-immune interaction chips, vascular-immune OoC, and lymph node-on-chip models where particulate-reduced media is preferred.
CAR-T & TIL Expansion
Low endotoxin (<0.05 EU/mL release specification) supports CAR-T manufacturing and TIL expansion protocols sensitive to LPS-driven activation artefacts.
Immune Cell Metabolic Flux
Defined RPMI base for ¹³C isotope tracing and LC-MS/NMR metabolomics of lymphocyte activation states. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.
Flow Cytometry & Confocal
Reduced particulate load supports flow cytometry and immune cell confocal imaging; contact us for phenol-red-free variants where autofluorescence must be minimized.
Analytical release specifications
Every lot released against the full specification matrix. CoA: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose (2 g/L) | [-] Sodium Pyruvate |
| Appearance | Red-colored, clear solution |
| Glucose | 2000 mg/L (2.0 g/L) |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 280–320 mOsm/kg H2O |
| Total ingredients | 40 components (see full composition tabs below) |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL (batch release specification) |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> Method 1 | NMT 25/mL |
| Particulate ≥25 µm USP <788> Method 1 | NMT 3/mL |
| Water purity | Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, protect from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO₂ requirement | 5% CO₂ required (sodium bicarbonate-buffered, ~24 mM NaHCO3) |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
RPMI 1640 w/o Sodium Pyruvate: 40 ingredients verified per lot with CAS numbers for full raw-material traceability. Contains reduced glutathione and a balanced profile of vitamins and amino acids optimized for lymphocyte and hematopoietic cell culture.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium nitrate tetrahydrate | 13477-34-4 | 100.000 |
| Magnesium sulfate anhydrous | 7487-88-9 | 48.840 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium bicarbonate | 144-55-8 | 2000.000 |
| Sodium chloride | 7647-14-5 | 6000.000 |
| Sodium phosphate dibasic anhydrous | 7558-79-4 | 800.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 10.000 |
| L-Arginine hydrochloride | 1119-34-2 | 241.000 |
| L-Asparagine | 70-47-3 | 50.000 |
| L-Aspartic acid | 56-84-8 | 20.000 |
| L-Cystine dihydrochloride | 30925-07-6 | 65.200 |
| L-Glutamic acid | 56-86-0 | 20.000 |
| L-Glutamine | 56-85-9 | 300.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 20.960 |
| L-Hydroxyproline | 51-35-4 | 20.000 |
| L-Isoleucine | 73-32-5 | 50.000 |
| L-Leucine | 61-90-5 | 50.000 |
| L-Lysine hydrochloride | 657-27-2 | 40.000 |
| L-Methionine | 63-68-3 | 15.000 |
| L-Phenylalanine | 63-91-2 | 15.000 |
| L-Proline | 147-85-3 | 20.000 |
| L-Serine | 56-45-1 | 30.000 |
| L-Threonine | 72-19-5 | 20.000 |
| L-Tryptophan | 73-22-3 | 5.000 |
| L-Tyrosine Disodium Salt | 69847-45-6 | 28.830 |
| L-Valine | 72-18-4 | 20.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 3.000 |
| D-Biotin | 58-85-5 | 0.200 |
| D-Ca-Pantothenate | 137-08-6 | 0.250 |
| Folic acid | 59-30-3 | 1.000 |
| Niacinamide | 98-92-0 | 1.000 |
| Pyridoxine hydrochloride | 58-56-0 | 1.000 |
| Riboflavin | 83-88-5 | 0.200 |
| Thiamine hydrochloride | 67-03-8 | 1.000 |
| Vitamin B12 | 68-19-9 | 0.005 |
| i-Inositol | 87-89-8 | 35.000 |
| p-Amino benzoic acid (PABA) | 150-13-0 | 1.000 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 2000.000 |
| Glutathione reduced | 70-18-8 | 1.000 |
| Phenol red sodium salt | 34487-61-1 | 5.300 |
Manufacturing & compliance
Every FluxMPS™ product is manufactured and released under a multi-layer quality system.
ISO 13485:2016 Quality Management
Manufactured under ISO 13485:2016-certified facilities. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm — trace-metal and organic-carbon (TOC) control during formulation.
ISO Class 5 Fill & Finish
Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.
Micro-Batch Precision
Small-batch, per-lot tested — no blending; Certificate of Analysis available for every lot.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
Endotoxin — USP <85> BET
LAL assay; release specification < 0.05 EU/mL per batch.
Particulate — USP <788> Method 1
Light obscuration; NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).
Osmolality — USP <785>
Target: 280–320 mOsm/kg H2O.
Documentation & CoA
Full CoA with raw-material traceability available on request.
How DCP-RPMI-P1X compares
FluxMPS™ DCP-RPMI-P1X vs. conventional 0.22 µm–filtered RPMI formulations.
| Parameter | DCP-RPMI-P1X (FluxMPS™) | Conventional RPMI 1640 (0.22 µm filtered) |
Standard DMEM/RPMI (0.22 µm filtered) |
|---|---|---|---|
| Grade | Microfluidics Suitable | Not specified | Not specified |
| RPMI 1640 without Sodium Pyruvate — researcher-defined secondary carbon for lymphocyte metabolic studies | check_circle Yes | cancel No | cancel No |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple-stage) | 1 | 1 |
| Mycoplasma-retentive filtration | check_circle Yes (0.1 µm stage) | cancel No | cancel No |
| Endotoxin (release specification) | FluxMPS™ — < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP <788> particulate tested (Method 1) | check_circle Yes | cancel No | cancel No |
| Water quality | Type 1, 18.2 MΩ·cm | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatible | check_circle Microfluidics Suitable | cancel Risk of clogging | cancel Risk of clogging |
| Custom formulation available | check_circle Yes | cancel No | cancel No |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ DCP-RPMI-P1X — RPMI 1640 w/o Sodium Pyruvate.
Supporting literature
Key publications supporting RPMI 1640 in immune cell culture and OoC applications.
- Moore GE, et al. Culture of normal human leukocytes. JAMA. 1967;199:519–524. doi:10.1083/jcb.1.3.273
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
- Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
