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FluxMPS™ RPMI 1640 Medium w/o Sodium Bicarbonate, Phenol Red: 1X Liquid

Product#: DCP-RPMI-BR1X
$34.10
DCP-RPMI-BR1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ RPMI 1640 Medium w/o Sodium Bicarbonate, Phenol Red: 1X Liquid

Contains L-Glutamine Contains Calcium Contains Magnesium Contains Glucose Contains Sodium Pyruvate Without Sodium Bicarbonate Without Phenol Red

FluxMPS™ DCP-RPMI-BR1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) RPMI 1640 formulation engineered for hematopoietic cells, lymphocytes and related immune-cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. Formulation: [+] 2.0 g/L Glucose, [+] L-Glutamine, [+] Sodium Pyruvate | [-] Sodium Bicarbonate, [-] Phenol Red.

  • Glucose source: 2000 mg/L (2.0 g/L) — complete carbon source, no supplementation required for glycolytic activity
  • Glutathione (reduced), 1.0 mg/L — antioxidant support for redox-sensitive immune cell types
  • Bicarbonate-free, phenol red-free formulation — CO₂-independent with optional HEPES buffering, and reduced optical background for fluorescence-based assays
  • Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final pore size
  • Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch
  • Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
  • 39 formulation components verified per lot — including 20 amino acids and 11 vitamins — with CAS-numbered raw-material traceability
  • Custom pH, nutrient concentration, HEPES and salt modifications available on request
CAT. NO.
DCP-RPMI-BR1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
RPMI 1640 w/o Sodium Bicarbonate, Phenol Red — 1X Liquid
  • Media familyRPMI 1640
  • Formulation[+] L-Glutamine, [+] Calcium, [+] Magnesium, [+] Glucose (2.0 g/L), [+] Sodium Pyruvate | [-] Sodium Bicarbonate, [-] Phenol Red
  • AppearancePale yellow-colored, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)230–270 mOsm/kg H₂O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
  • CO₂ requirementCO₂-independent; HEPES supplementation recommended
  • Storage2–8°C, protected from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered RPMI passes subvisible particulates, mycoplasma-scale contaminants, and endotoxin fragments that can activate TLR4 and confound immune cell assays. FluxMPS™ is built for these failure modes.

filter_alt

Microchannel-safe purity

0.04 µm final filtration with USP <788> Method 1 (light obscuration) particulate testing; particle-free media suited to suspension immune-cell OoC and flow cytometry.

target

Total metabolic control

Defined glucose (2.0 g/L), L-glutamine and sodium pyruvate levels support Warburg-effect and lymphocyte metabolic-flux studies on a consistent formulation baseline.

water_drop

Ultrapure-grade water

Type 1 water (18.2 MΩ·cm) with controlled trace-metal and organic-carbon content minimizes reagent-introduced variability in sensitive immune cell assays.

visibility

Low background for imaging

0.04 µm filtration maintains an ultra-low particulate baseline suited to confocal microscopy and biosensor-based readouts. Phenol red is not included in this formulation.

science

Rich, stable nutrient profile

39 formulation components, including glutathione (reduced) and a balanced amino acid/vitamin profile optimized for lymphocyte and hematopoietic cell culture, released per lot.

tune

Customization on demand

pH, nutrient concentrations, HEPES, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages — two dedicated 0.1 µm prefilter + 0.04 µm final-filter pairs in series — reaching a validated 0.04 µm final pore size under aseptic fill conditions.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulate, cell debris and protein aggregates; protects the first 0.04 µm final-filter cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and mycoplasma-scale contaminants (0.2–0.3 µm) that pass a standard 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm final-filter cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill and finish.

Performance vs. conventional media

5×
Cleaner than 0.22 µm media by particulate count
0.04
µm Final pore size — sub-mycoplasma polishing
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma control is by 0.1 µm and 0.04 µm mycoplasma-retentive filtration at every production stage (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-RPMI-BR1X RPMI 1640 quadruple-stage filtration system diagram, 0.1 micron times 2 plus 0.04 micron times 2, for organ-on-a-chip and microfluidic immune cell applications, Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-RPMI-BR1X
Applications

Immune cell OoC and hematopoietic applications

FluxMPS™ DCP-RPMI-BR1X — RPMI 1640 — delivers 0.04 µm filtered purity for hematopoietic cells and related OoC applications.

Automated Bioreactors & Robotics

Next-Generation System Uptime

Optional 0.01 µm (10 nm) MPS Grade ultra-filtered variant available on request for automated bioreactor and robotic perfusion systems.

  • Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
  • Valve & Sensor Protection: Eliminates micro-fouling in automated perfusion systems
  • Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Immunology

T Cell & Lymphocyte Culture

RPMI 1640 is the standard base for primary T cells, B cells, NK cells, and monocytes. FluxMPS™ 0.04 µm filtration reduces particulate load relevant to TLR4-sensitive immune assays.

T cellsB cellsNK cellsPBMC
Cancer Biology

Leukemia & Lymphoma Lines

RPMI supports Jurkat, Raji, K562, HL-60, and other hematopoietic cancer lines where DMEM would alter proliferation and signaling behavior.

JurkatRajiK562HL-60
Microfluidics

Immune Cell OoC

0.04 µm filtered RPMI for tumor-immune interaction chips, vascular-immune OoC, and lymph-node-on-chip models built on microphysiological system (MPS) platforms.

Tumor-immune chipLymph node OoCMPS
Immunotherapy

CAR-T & TIL Expansion

A low endotoxin release specification (< 0.05 EU/mL) supports CAR-T manufacturing and TIL expansion protocols sensitive to endotoxin-driven activation artefacts.

CAR-TTILTCR-T
Metabolomics

Immune Cell Metabolic Flux

Bicarbonate-free, phenol red-free RPMI base compatible with Seahorse XF T cell metabolic assays, glycolysis stress tests, and ¹³C isotope tracing of lymphocyte activation states.

Seahorse XF¹³C tracingGlycolysis
Live-Cell Imaging

Flow Cytometry & Confocal

Ultra-low particulate, phenol red-free formulation reduces optical background for PE-channel flow cytometry and immune cell confocal imaging.

Flow cytometryConfocalELISA
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] L-Glutamine, [+] Calcium, [+] Magnesium, [+] Glucose (2.0 g/L), [+] Sodium Pyruvate | [-] Sodium Bicarbonate, [-] Phenol Red
Appearance Pale yellow-colored, clear solution
Glucose 2000 mg/L (2.0 g/L)
pH USP <791> 7.4
Osmolality USP <785> 230–270 mOsm/kg H₂O
Total ingredients 39 components across 4 categories (Inorganic Salts, Amino Acids, Vitamins, Others)
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (per-batch release; see Manufacturing & Compliance)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm / 0.04 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO₂ requirement CO₂-independent; supplement with HEPES (15–25 mM) for pH buffering
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)

Available pack sizes: 500 mL, 1000 mL

Formulation

Full composition (mg/L)

RPMI 1640: 39 ingredients verified per lot with CAS numbers for full raw-material traceability. Contains glutathione (reduced) and a balanced profile of vitamins and amino acids optimized for lymphocyte and hematopoietic cell culture.

Component CAS Number mg/L
INORGANIC SALTS
Calcium nitrate tetrahydrate 13477-34-4 100.000
Magnesium sulfate anhydrous 7487-88-9 48.840
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6000.000
Sodium phosphate dibasic anhydrous 7558-49-4 800.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 10.000
L-Arginine hydrochloride 1119-34-2 241.000
L-Asparagine 70-47-3 50.000
L-Aspartic acid 56-84-8 20.000
L-Cystine dihydrochloride 30925-07-6 65.200
L-Glutamic acid 56-86-0 20.000
L-Glutamine 56-85-9 300.000
L-Histidine hydrochloride monohydrate 5934-29-2 20.960
L-Hydroxyproline 51-35-4 20.000
L-Isoleucine 73-32-5 50.000
L-Leucine 61-90-5 50.000
L-Lysine hydrochloride 657-27-2 40.000
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 15.000
L-Proline 147-85-3 20.000
L-Serine 56-45-1 30.000
L-Threonine 72-19-5 20.000
L-Tryptophan 73-22-3 5.000
L-Tyrosine Disodium Salt 69847-45-6 28.830
L-Valine 72-18-4 20.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 3.000
D-Biotin 58-85-5 0.200
D-Ca-Pantothenate 137-08-6 0.250
Folic acid 59-30-3 1.000
Niacinamide 98-92-0 1.00
Pyridoxine hydrochloride 58-56-0 1.00
Riboflavin 83-88-5 0.200
Thiamine hydrochloride 67-03-8 1.000
Vitamin B12 68-19-9 0.005
i-Inositol 87-89-8 35.000
p-Amino benzoic acid (PABA) 150-13-0 1.000
OTHERS
D-Glucose 50-99-7 2000.000
Glutathione reduced 70-18-8 1.000
Sodium pyruvate 113-24-6 110.000
Custom formulation: Contact support@diagnocine.com for DCP-RPMI-BR1X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016-certified quality management system. Final QC at Diagnocine, Totowa, NJ, USA.

filter_alt

Quadruple-Stage Filtration

Two dedicated 0.1 µm/0.04 µm prefilter and final-filter pairs in series, reaching a 0.04 µm final pore size.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested; Certificate of Analysis available for every batch.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL per batch.

Particulate — USP <788> Method 1

Light obscuration; NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 230–270 mOsm/kg H₂O.

Documentation & CoA

Full CoA with raw-material traceability available on request.

Certificate of Analysis: Request for any DCP-RPMI-BR1X batch at support@diagnocine.com.
Product Comparison

How DCP-RPMI-BR1X compares

FluxMPS™ DCP-RPMI-BR1X vs. conventional 0.22 µm–filtered RPMI formulations.

Parameter DCP-RPMI-BR1X (FluxMPS™) Conventional RPMI 1640
(0.22 µm filtered)		 Standard DMEM/RPMI
(0.22 µm filtered)
Grade Microfluidics Suitable Standard research grade Standard research grade
RPMI 1640 without Sodium Bicarbonate and Phenol Red — CO₂-free, reduced-background immune cell base check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma-retentive filtration check_circle Yes (0.1/0.04 µm) cancel No cancel No
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> Method 1 particulate tested check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatibility check_circle Microfluidics Suitable cancel Risk of clogging cancel Risk of clogging
Custom formulation Available on request Not typically offered Not typically offered

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as “Not specified”.

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-RPMI-BR1X — RPMI 1640.

Yes. DCP-RPMI-BR1X is processed through a Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final pore size, delivering ultra-low particulate levels appropriate for microfluidic channels, OoC and MPS platforms. Its bicarbonate-free, phenol red-free RPMI 1640 base with sub-mycoplasma filtration purity is suited to immune cell OoC, tumor-immune interaction chips, and lymphocyte perfusion models.
FluxMPS™ uses four sequential filters — two 0.1 µm/0.04 µm prefilter and final-filter pairs in series — reaching a 0.04 µm final pore size. This retains particulates and mycoplasma-scale contaminants (0.2–0.3 µm) that a standard 0.22 µm filter does not, delivering approximately 5× lower particulate counts by count.
This formulation is bicarbonate-free and phenol red-free, making it CO₂-independent and lower in optical background for fluorescence-based assays. For applications requiring active pH buffering outside a sealed system, supplement with HEPES (15–25 mM). Glucose (2.0 g/L), L-glutamine and sodium pyruvate are already included and do not require supplementation.
No. This formulation is bicarbonate-free and CO₂-independent; supplement with HEPES (15–25 mM) for pH buffering in open or atmospheric culture systems.
Yes. FBS (5–10%), serum-free supplements, growth factors, or antibiotics may be added. Pre-filter serum-containing additions through a 0.2 µm low-protein-binding PES or PVDF filter before addition; do not use a 0.04 µm filter for serum, as it retains immunoglobulins and lipoproteins. Contact support@diagnocine.com for custom co-formulation.
FluxMPS™ DCP-RPMI-BR1X carries a release specification of < 0.05 EU/mL, tested by LAL assay (USP <85> Bacterial Endotoxins Test) on every manufacturing batch prior to release. For immune cell assays, endotoxin can activate TLR4/NF-κB signaling and confound lymphocyte activation and cytokine measurements independent of experimental treatment, which is why this specification is controlled at the batch level.
Yes. A Certificate of Analysis is available for every batch, covering appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma-retentive filtration status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting RPMI 1640 in immune cell culture and OoC applications.

  1. Moore GE, et al. Culture of normal human leukocytes. JAMA. 1967;199:519–524. doi:10.1083/jcb.1.3.273
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  6. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  7. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  8. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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