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FluxMPS™ RPMI 1640 Medium w/o Phenol Red: 1X Liquid

Product#: DCP-RPMI-R1X
$34.10
DCP-RPMI-R1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ RPMI 1640 Medium w/o Phenol Red

Contains L-Glutamine Contains Sodium Bicarbonate Contains Calcium Contains Magnesium Contains 2 g/L Glucose Contains Sodium Pyruvate Without Phenol Red

FluxMPS™ DCP-RPMI-R1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) RPMI 1640 formulation without phenol red, engineered for lymphocyte, hematopoietic and immune cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

  • Glucose source: 2000 mg/L (2.0 g/L) D-glucose for standard RPMI energy metabolism
  • L-Glutamine 300 mg/L and Sodium Pyruvate 110 mg/L support lymphocyte and hematopoietic cell energy demands
  • Glutathione (reduced, 1.0 mg/L) included — antioxidant support for redox-sensitive immune cell types
  • Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final pore size for sub-mycoplasma-range purity
  • Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85>) — relevant to TLR4-sensitive immune cell assays
  • Phenol red excluded — eliminates 520–560 nm autofluorescence for flow cytometry, confocal imaging and hormone-sensitive assays
  • Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa NJ
  • Custom pH, nutrient concentration and HEPES buffering available on request
CAT. NO.
DCP-RPMI-R1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
RPMI 1640 w/o Phenol Red — 1X Liquid
  • Media familyRPMI 1640
  • Glucose2000 mg/L (2.0 g/L)
  • L-Glutamine300 mg/L
  • Sodium Pyruvate110 mg/L
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)280–320 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered RPMI passes mycoplasma-scale particles, subvisible particulates, and phenol red autofluorescence that confound immune cell assays. FluxMPS™ is built to remove these failure modes at the filtration and formulation level.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> Method 1 (light obscuration) particulate compliance. Particle-controlled media for suspension immune cell OoC and flow cytometry.

science

Immune cell–optimized formulation

RPMI 1640 contains reduced glutathione and a balanced amino acid / vitamin profile suited to lymphocyte and hematopoietic cell culture.

water_drop

Purity-controlled water

Manufactured with trace-metal and total organic carbon (TOC)-controlled process water as part of the ISO 13485:2016 quality system.

visibility

Low background for imaging

Phenol red excluded from this formulation, reducing baseline particulate and optical interference for confocal and PE-channel flow cytometry work.

analytics

Rich nutrient profile

RPMI's specialized amino acid and vitamin profile is delivered through a 0.04 µm final-filtered process for OoC use.

tune

Customization on demand

pH, nutrient concentrations, HEPES, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration passes — two dedicated prefilter + final-filter pairs — reaching a final 0.04 µm polish.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulate, cell debris and protein aggregates; protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a standard 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm cartridge from particulate load.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter prior to aseptic fill & finish.

Performance vs. conventional media

5×
Cleaner than 0.22 µm media by particulate count
0.04
µm Final pore size across 4 filtration passes
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma control is by 0.1 µm mycoplasma-retentive filtration (not tested per lot); mycoplasma organisms are approximately 0.2–0.3 µm in diameter.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-RPMI-R1X RPMI 1640 without phenol red quadruple-stage filtration system 0.1 micron x2 plus 0.04 micron x2 for organ-on-a-chip and microfluidic cell culture applications, Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-RPMI-R1X
Applications

Immune cell OoC and hematopoietic applications

FluxMPS™ DCP-RPMI-R1X — RPMI 1640 w/o phenol red — delivers 0.04 µm filtered, Microfluidics Suitable purity for lymphocyte, hematopoietic and cancer cell OoC applications.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available on request for automated perfusion and robotic handling systems.

  • Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates
  • Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion systems
  • Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Immunology

T Cell & Lymphocyte Culture

RPMI 1640 is the standard for primary T cells, B cells, NK cells, and monocytes. 0.04 µm filtration reduces particulate load relevant to immune assay signal.

T cellsB cellsNK cellsPBMC
Cancer Biology

Leukemia & Lymphoma Lines

RPMI supports NCI-60 cancer lines, Jurkat, Raji, K562, HL-60, and hematopoietic cancer lines where DMEM would alter proliferation and signaling.

JurkatRajiK562HL-60
Microfluidics

Immune Cell OoC

0.04 µm filtered RPMI for tumor-immune interaction chips, vascular-immune OoC, and lymph node-on-chip microphysiological systems (MPS).

Tumor-immune chipLymph node OoCMPS
Immunotherapy

CAR-T & TIL Expansion

Endotoxin release specification < 0.05 EU/mL is relevant to CAR-T manufacturing and TIL expansion protocols sensitive to TLR4 activation.

CAR-TTILTCR-T
Metabolomics

Immune Cell Metabolic Flux

Defined RPMI base for glycolysis stress tests and ¹³C isotope tracing of lymphocyte activation states. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.

¹³C tracingGlycolysisNMR metabolomics
Live-Cell Imaging

Flow Cytometry & Confocal

Phenol red–free formulation reduces autofluorescence for PE-channel flow cytometry and immune cell confocal imaging.

Flow cytometryConfocalELISA
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. Pack sizes: 500 mL, 1000 mL. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Calcium, [+] Magnesium, [+] 2 g/L Glucose, [+] Sodium Pyruvate | [-] Phenol Red
Appearance Colorless to pale yellow, clear solution
Glucose 2000 mg/L (2.0 g/L)
pH USP <791> 7.4
Osmolality USP <785> 280–320 mOsm/kg H2O
Total ingredients 40
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement 5% CO2 required (standard RPMI 1640 bicarbonate buffering system)
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

RPMI 1640: 40 ingredients verified per lot with CAS numbers for raw-material traceability. Contains reduced glutathione and a balanced amino acid / vitamin profile suited to lymphocyte and hematopoietic cell culture.

Component CAS Number mg/L
INORGANIC SALTS
Calcium nitrate tetrahydrate 13477-34-4 100.000
Magnesium sulfate anhydrous 7487-88-9 48.840
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 2000.000
Sodium chloride 7647-14-5 6000.00
Sodium phosphate dibasic anhydrous 7558-79-4 800.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 10.000
L-Arginine hydrochloride 1119-34-2 241.000
L-Asparagine 70-47-3 50.000
L-Aspartic acid 56-84-8 20.000
L-Cystine dihydrochloride 30925-07-6 65.200
L-Glutamic acid 56-86-0 20.000
L-Glutamine 56-85-9 300.000
L-Histidine hydrochloride monohydrate 5934-29-2 20.960
L-Hydroxyproline 51-35-4 20.000
L-Isoleucine 73-32-5 50.000
L-Leucine 61-90-5 50.000
L-Lysine hydrochloride 657-27-2 40.000
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 15.000
L-Proline 147-85-3 20.000
L-Serine 56-45-1 30.000
L-Threonine 72-19-5 20.000
L-Tryptophan 73-22-3 5.000
L-Tyrosine Disodium Salt 69847-45-6 28.830
L-Valine 72-18-4 20.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 3.000
D-Biotin 58-85-5 0.200
D-Ca-Pantothenate 137-08-6 0.250
Folic acid 59-30-3 1.000
Niacinamide 98-92-0 1.000
Pyridoxine hydrochloride 58-56-0 1.000
Riboflavin 83-88-5 0.200
Thiamine hydrochloride 67-03-8 1.000
Vitamin B12 68-19-9 0.005
i-Inositol 87-89-8 35.000
p-Amino benzoic acid (PABA) 150-13-0 1.000
OTHERS
D-Glucose 50-99-7 2000.000
Glutathione reduced 70-18-8 1.000
Sodium Pyruvate 113-24-6 110.000
Custom formulation: Contact support@diagnocine.com for DCP-RPMI-R1X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product manufactured and released under a rigorous multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016-certified quality management system. Final QC at Diagnocine R&D Center, Totowa, NJ, USA.

filter_alt

Quadruple-Stage Filtration

0.1 µm ×2 + 0.04 µm ×2 filtration train applied to every batch.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — Certificate of Analysis available for every lot.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL per batch.

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 280–320 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-RPMI-R1X compares

FluxMPS™ DCP-RPMI-R1X vs. conventional 0.22 µm–filtered RPMI formulations.

Parameter DCP-RPMI-R1X (FluxMPS™) Conventional RPMI 1640
(0.22 µm filtered)		 Standard DMEM/RPMI
(0.22 µm filtered)
Grade Microfluidics Suitable Not designated Not designated
RPMI 1640 without Phenol Red — autofluorescence-reduced for flow cytometry, confocal, and hormone-sensitive assays check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma barrier filtration check_circle Yes (0.1 µm) cancel No cancel No
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate tested check_circle Yes (Method 1) cancel Not specified cancel Not specified
Manufacturing QMS ISO 13485:2016 Not specified Not specified
Microfluidic channel compatibility check_circle Microfluidics Suitable (0.04 µm) cancel Risk of clogging cancel Risk of clogging
Custom formulation available check_circle Yes cancel No cancel No

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-RPMI-R1X — RPMI 1640 w/o Phenol Red.

Yes. DCP-RPMI-R1X is processed through a Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final pore size, making it Microfluidics Suitable for immune cell OoC, tumor-immune interaction chips, and lymphocyte perfusion models.
FluxMPS™ uses four sequential passes — 0.1 µm prefiltration I, 0.04 µm final filtration I, 0.1 µm prefiltration II, and 0.04 µm final filtration II (polish) — reaching a 0.04 µm final pore size versus the 0.22 µm typical of conventional media, delivering approximately 5× fewer particles by count.
Phenol red is removed to eliminate 520–560 nm autofluorescence and its weak estrogenic activity. This is important for lymphocyte flow cytometry (PE channel interference), immune cell confocal imaging, hormone-sensitive cancer cell assays (ER+ breast cancer, endocrine tumors), and ELISA-based assays where phenol red absorbance interferes. This formulation already includes glucose, L-glutamine and sodium pyruvate; no additional carbon-source supplementation is required for standard use.
5% CO2 required (standard RPMI 1640 bicarbonate buffering system).
Yes. FBS (5–10%), serum-free supplements, growth factors, or antibiotics may be added as required. When adding serum or protein-containing supplements, filter through a 0.2 µm low-protein-binding PES or PVDF membrane — never 0.04 µm, which retains IgM, lipoproteins and much of the functional serum fraction. Contact support@diagnocine.com for custom co-formulation.
Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested by LAL assay (USP <85>, assay sensitivity 0.005 EU/mL) and must meet the release specification of < 0.05 EU/mL before release. For immune cell work, endotoxin can activate TLR4/NF-κB signaling and confound lymphocyte activation studies independent of experimental treatment.
Yes. Full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma filtration control, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting RPMI 1640 in immune cell culture and OoC applications.

  1. Moore GE, et al. Culture of normal human leukocytes. JAMA. 1967;199:519–524. doi:10.1083/jcb.1.3.273
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  6. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  7. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  8. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
  9. Gehring MA, et al. Immune-cell microphysiological systems for lymphocyte biology. Trends Biotechnol. 2019;37:591–603. doi:10.1016/j.tibtech.2018.11.001

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